Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz

Autores
Ordoñez, Alicia Lucia; Gómez, José D.; Cudmani, Norma Mercedes; Vattuone, Marta Amelia; Isla, Maria Ines
Año de publicación
2003
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The antimicrobial properties of Sechium edule (Jacq) Swartz alcoholic extracts obtained according to the Farmacopea Argentina (6th edn) were tested against bacteria of clinical relevance as nosocomial pathogens. To evaluate antibacterial activity, the disc diffusion assay was carried out with several gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus pyogenes, Streptococcus agalactiae, Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212). This assay was suitable for the screening of a large number of extracts at one time. All ethanolic extracts showed activity against gram-positive bacteria. Minimal inhibitory concentration (MIC) values were determined with a microdilution assay. The highest activity was obtained with the 80% aqueous-ethanolic leaf extract (MIC values of 4.16/8.32 mg/ml against staphylococci and enterococci) and with the 96% ethanolic seed extract (MIC values of 8.32/16.64 mg/ml and /8.32 mg/ml against staphylococci and enterococci, respectively). The results indicate that both fluid extract and tincture have very good antimicrobial efficacy against all strains of multiresistant staphylococci and enterococci. In this study the minimal bactericidal concentration (MBC) values were identical to the MIC values or twofold higher than the corresponding MIC. This may indicate a bactericidal effect. Stored extracts have similar anti-staphylococcal and anti-enterococcal activity to recently obtained extracts. The results obtained might be considered sufficient to warrant further studies aimed at isolation and identification of the active principle.
Fil: Ordoñez, Alicia Lucia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Gómez, José D.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cudmani, Norma Mercedes. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Hospital de Clínicas "Dr. Nicolás Avellaneda"; Argentina. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Departamento Bioquímico; Argentina
Fil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina
Materia
SECHIUM EDULE
ANTIMICROBIAL ACTIVITY
PAPA DEL AIRE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105647
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/105647
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antimicrobial activity of nine extracts of sechium edule (Jacq) swartzOrdoñez, Alicia LuciaGómez, José D.Cudmani, Norma MercedesVattuone, Marta AmeliaIsla, Maria InesSECHIUM EDULEANTIMICROBIAL ACTIVITYPAPA DEL AIREhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The antimicrobial properties of Sechium edule (Jacq) Swartz alcoholic extracts obtained according to the Farmacopea Argentina (6th edn) were tested against bacteria of clinical relevance as nosocomial pathogens. To evaluate antibacterial activity, the disc diffusion assay was carried out with several gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus pyogenes, Streptococcus agalactiae, Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212). This assay was suitable for the screening of a large number of extracts at one time. All ethanolic extracts showed activity against gram-positive bacteria. Minimal inhibitory concentration (MIC) values were determined with a microdilution assay. The highest activity was obtained with the 80% aqueous-ethanolic leaf extract (MIC values of 4.16/8.32 mg/ml against staphylococci and enterococci) and with the 96% ethanolic seed extract (MIC values of 8.32/16.64 mg/ml and /8.32 mg/ml against staphylococci and enterococci, respectively). The results indicate that both fluid extract and tincture have very good antimicrobial efficacy against all strains of multiresistant staphylococci and enterococci. In this study the minimal bactericidal concentration (MBC) values were identical to the MIC values or twofold higher than the corresponding MIC. This may indicate a bactericidal effect. Stored extracts have similar anti-staphylococcal and anti-enterococcal activity to recently obtained extracts. The results obtained might be considered sufficient to warrant further studies aimed at isolation and identification of the active principle.Fil: Ordoñez, Alicia Lucia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Gómez, José D.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; ArgentinaFil: Cudmani, Norma Mercedes. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Hospital de Clínicas "Dr. Nicolás Avellaneda"; Argentina. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Departamento Bioquímico; ArgentinaFil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; ArgentinaTaylor & Francis2003-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105647Ordoñez, Alicia Lucia; Gómez, José D.; Cudmani, Norma Mercedes; Vattuone, Marta Amelia; Isla, Maria Ines; Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz; Taylor & Francis; Microbial Ecology in Health and Disease; 15; 1; 7-2003; 33-390891-060X1651-2235CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1080/0891060010015583info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/0891060010015583info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:24:28Zoai:ri.conicet.gov.ar:11336/105647instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:24:28.825CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz
title Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz
spellingShingle Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz
Ordoñez, Alicia Lucia
SECHIUM EDULE
ANTIMICROBIAL ACTIVITY
PAPA DEL AIRE
title_short Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz
title_full Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz
title_fullStr Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz
title_full_unstemmed Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz
title_sort Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz
dc.creator.none.fl_str_mv Ordoñez, Alicia Lucia
Gómez, José D.
Cudmani, Norma Mercedes
Vattuone, Marta Amelia
Isla, Maria Ines
author Ordoñez, Alicia Lucia
author_facet Ordoñez, Alicia Lucia
Gómez, José D.
Cudmani, Norma Mercedes
Vattuone, Marta Amelia
Isla, Maria Ines
author_role author
author2 Gómez, José D.
Cudmani, Norma Mercedes
Vattuone, Marta Amelia
Isla, Maria Ines
author2_role author
author
author
author
dc.subject.none.fl_str_mv SECHIUM EDULE
ANTIMICROBIAL ACTIVITY
PAPA DEL AIRE
topic SECHIUM EDULE
ANTIMICROBIAL ACTIVITY
PAPA DEL AIRE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The antimicrobial properties of Sechium edule (Jacq) Swartz alcoholic extracts obtained according to the Farmacopea Argentina (6th edn) were tested against bacteria of clinical relevance as nosocomial pathogens. To evaluate antibacterial activity, the disc diffusion assay was carried out with several gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus pyogenes, Streptococcus agalactiae, Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212). This assay was suitable for the screening of a large number of extracts at one time. All ethanolic extracts showed activity against gram-positive bacteria. Minimal inhibitory concentration (MIC) values were determined with a microdilution assay. The highest activity was obtained with the 80% aqueous-ethanolic leaf extract (MIC values of 4.16/8.32 mg/ml against staphylococci and enterococci) and with the 96% ethanolic seed extract (MIC values of 8.32/16.64 mg/ml and /8.32 mg/ml against staphylococci and enterococci, respectively). The results indicate that both fluid extract and tincture have very good antimicrobial efficacy against all strains of multiresistant staphylococci and enterococci. In this study the minimal bactericidal concentration (MBC) values were identical to the MIC values or twofold higher than the corresponding MIC. This may indicate a bactericidal effect. Stored extracts have similar anti-staphylococcal and anti-enterococcal activity to recently obtained extracts. The results obtained might be considered sufficient to warrant further studies aimed at isolation and identification of the active principle.
Fil: Ordoñez, Alicia Lucia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Gómez, José D.. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
Fil: Cudmani, Norma Mercedes. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Sistema Provincial de Salud. Hospital de Clínicas "Dr. Nicolás Avellaneda"; Argentina. Gobierno de la Provincia de Tucumán. Ministerio de Salud. Departamento Bioquímico; Argentina
Fil: Vattuone, Marta Amelia. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; Argentina
Fil: Isla, Maria Ines. Universidad Nacional de Tucumán. Instituto de Bioprospección y Fisiología Vegetal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet Noa Sur. Instituto de Bioprospección y Fisiología Vegetal; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Estudios Vegetales; Argentina
description The antimicrobial properties of Sechium edule (Jacq) Swartz alcoholic extracts obtained according to the Farmacopea Argentina (6th edn) were tested against bacteria of clinical relevance as nosocomial pathogens. To evaluate antibacterial activity, the disc diffusion assay was carried out with several gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus, coagulase-negative staphylococci, Streptococcus pyogenes, Streptococcus agalactiae, Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212). This assay was suitable for the screening of a large number of extracts at one time. All ethanolic extracts showed activity against gram-positive bacteria. Minimal inhibitory concentration (MIC) values were determined with a microdilution assay. The highest activity was obtained with the 80% aqueous-ethanolic leaf extract (MIC values of 4.16/8.32 mg/ml against staphylococci and enterococci) and with the 96% ethanolic seed extract (MIC values of 8.32/16.64 mg/ml and /8.32 mg/ml against staphylococci and enterococci, respectively). The results indicate that both fluid extract and tincture have very good antimicrobial efficacy against all strains of multiresistant staphylococci and enterococci. In this study the minimal bactericidal concentration (MBC) values were identical to the MIC values or twofold higher than the corresponding MIC. This may indicate a bactericidal effect. Stored extracts have similar anti-staphylococcal and anti-enterococcal activity to recently obtained extracts. The results obtained might be considered sufficient to warrant further studies aimed at isolation and identification of the active principle.
publishDate 2003
dc.date.none.fl_str_mv 2003-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105647
Ordoñez, Alicia Lucia; Gómez, José D.; Cudmani, Norma Mercedes; Vattuone, Marta Amelia; Isla, Maria Ines; Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz; Taylor & Francis; Microbial Ecology in Health and Disease; 15; 1; 7-2003; 33-39
0891-060X
1651-2235
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105647
identifier_str_mv Ordoñez, Alicia Lucia; Gómez, José D.; Cudmani, Norma Mercedes; Vattuone, Marta Amelia; Isla, Maria Ines; Antimicrobial activity of nine extracts of sechium edule (Jacq) swartz; Taylor & Francis; Microbial Ecology in Health and Disease; 15; 1; 7-2003; 33-39
0891-060X
1651-2235
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1080/0891060010015583
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/abs/10.1080/0891060010015583
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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