Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E

Autores
Risso, Analía Lorena; Pellegrino, Francisco Javier; Corrada, Yanina Alejandra; Schinella, Guillermo Raúl
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) supplementation on total antioxidant activity of dog seminal plasma, and further assessed oxidative stress. Additionally, we measured the effect of this supplementation on hematological parameters and serum biochemistry. Materials and Methods: In this experimental study, six male dogs were assigned to one of the following three groups for a period of 60 days using a replicated 3×3 Latin square design: control (CG), FO (FOG) and FO in addition to VE (FOEG). On days 0 and 60 of the trial, semen and blood samples were obtained. 2,2V-azino-bis (3-ethylbenzothiazoline- 6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine total antioxidant activity. Oxidative stress was determined by measuring total sulfhydryl group (T-SH). Results: Dogs supplemented with FO alone had a lower total antioxidant activity in seminal plasma (ABTS: -59.86% vs.CG and -57.3% vs. FOEG; and FRAP: -37.3% vs. CG and -40.5% vs. FOEG), and higher oxidative stress (T-SH: +53.0% vs. CG and+60.2% vs. FOEG) compared with the other two groups (P<0.05). Serum triglyceride (TG) concentration decreased in FOG and FOEG compared with CG, on day 60 (P<0.01). Conclusion: We concluded that total antioxidant activitydecreased and oxidative stress increased in seminal plasma of dogs after FO supplementation for 60 days.
Instituto de Genética Veterinaria
Facultad de Ciencias Médicas
Materia
Ciencias Veterinarias
Antioxidants
Fish Oil
Oxidative Stress
Vitamin E
Dog
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/118871

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spelling Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin ERisso, Analía LorenaPellegrino, Francisco JavierCorrada, Yanina AlejandraSchinella, Guillermo RaúlCiencias VeterinariasAntioxidantsFish OilOxidative StressVitamin EDogBackground: We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) supplementation on total antioxidant activity of dog seminal plasma, and further assessed oxidative stress. Additionally, we measured the effect of this supplementation on hematological parameters and serum biochemistry. Materials and Methods: In this experimental study, six male dogs were assigned to one of the following three groups for a period of 60 days using a replicated 3×3 Latin square design: control (CG), FO (FOG) and FO in addition to VE (FOEG). On days 0 and 60 of the trial, semen and blood samples were obtained. 2,2V-azino-bis (3-ethylbenzothiazoline- 6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine total antioxidant activity. Oxidative stress was determined by measuring total sulfhydryl group (T-SH). Results: Dogs supplemented with FO alone had a lower total antioxidant activity in seminal plasma (ABTS: -59.86% vs.CG and -57.3% vs. FOEG; and FRAP: -37.3% vs. CG and -40.5% vs. FOEG), and higher oxidative stress (T-SH: +53.0% vs. CG and+60.2% vs. FOEG) compared with the other two groups (P<0.05). Serum triglyceride (TG) concentration decreased in FOG and FOEG compared with CG, on day 60 (P<0.01). Conclusion: We concluded that total antioxidant activitydecreased and oxidative stress increased in seminal plasma of dogs after FO supplementation for 60 days.Instituto de Genética VeterinariaFacultad de Ciencias Médicas2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf15-19http://sedici.unlp.edu.ar/handle/10915/118871enginfo:eu-repo/semantics/altIdentifier/issn/2008-076Xinfo:eu-repo/semantics/altIdentifier/issn/2008-077info:eu-repo/semantics/altIdentifier/doi/10.22074/IJFS.2021.6226info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/3.0/Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:27:59Zoai:sedici.unlp.edu.ar:10915/118871Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:28:00.083SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E
title Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E
spellingShingle Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E
Risso, Analía Lorena
Ciencias Veterinarias
Antioxidants
Fish Oil
Oxidative Stress
Vitamin E
Dog
title_short Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E
title_full Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E
title_fullStr Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E
title_full_unstemmed Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E
title_sort Evaluation of Total Antioxidant Activity and Oxidative Stress in Seminal Plasma from Dogs Supplemented with Fish Oil and Vitamin E
dc.creator.none.fl_str_mv Risso, Analía Lorena
Pellegrino, Francisco Javier
Corrada, Yanina Alejandra
Schinella, Guillermo Raúl
author Risso, Analía Lorena
author_facet Risso, Analía Lorena
Pellegrino, Francisco Javier
Corrada, Yanina Alejandra
Schinella, Guillermo Raúl
author_role author
author2 Pellegrino, Francisco Javier
Corrada, Yanina Alejandra
Schinella, Guillermo Raúl
author2_role author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Antioxidants
Fish Oil
Oxidative Stress
Vitamin E
Dog
topic Ciencias Veterinarias
Antioxidants
Fish Oil
Oxidative Stress
Vitamin E
Dog
dc.description.none.fl_txt_mv Background: We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) supplementation on total antioxidant activity of dog seminal plasma, and further assessed oxidative stress. Additionally, we measured the effect of this supplementation on hematological parameters and serum biochemistry. Materials and Methods: In this experimental study, six male dogs were assigned to one of the following three groups for a period of 60 days using a replicated 3×3 Latin square design: control (CG), FO (FOG) and FO in addition to VE (FOEG). On days 0 and 60 of the trial, semen and blood samples were obtained. 2,2V-azino-bis (3-ethylbenzothiazoline- 6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine total antioxidant activity. Oxidative stress was determined by measuring total sulfhydryl group (T-SH). Results: Dogs supplemented with FO alone had a lower total antioxidant activity in seminal plasma (ABTS: -59.86% vs.CG and -57.3% vs. FOEG; and FRAP: -37.3% vs. CG and -40.5% vs. FOEG), and higher oxidative stress (T-SH: +53.0% vs. CG and+60.2% vs. FOEG) compared with the other two groups (P<0.05). Serum triglyceride (TG) concentration decreased in FOG and FOEG compared with CG, on day 60 (P<0.01). Conclusion: We concluded that total antioxidant activitydecreased and oxidative stress increased in seminal plasma of dogs after FO supplementation for 60 days.
Instituto de Genética Veterinaria
Facultad de Ciencias Médicas
description Background: We evaluated the effect of fish oil (FO) and FO in addition to vitamin E (VE) supplementation on total antioxidant activity of dog seminal plasma, and further assessed oxidative stress. Additionally, we measured the effect of this supplementation on hematological parameters and serum biochemistry. Materials and Methods: In this experimental study, six male dogs were assigned to one of the following three groups for a period of 60 days using a replicated 3×3 Latin square design: control (CG), FO (FOG) and FO in addition to VE (FOEG). On days 0 and 60 of the trial, semen and blood samples were obtained. 2,2V-azino-bis (3-ethylbenzothiazoline- 6-sulfonate) (ABTS) and ferric reducing antioxidant power (FRAP) assays were used to determine total antioxidant activity. Oxidative stress was determined by measuring total sulfhydryl group (T-SH). Results: Dogs supplemented with FO alone had a lower total antioxidant activity in seminal plasma (ABTS: -59.86% vs.CG and -57.3% vs. FOEG; and FRAP: -37.3% vs. CG and -40.5% vs. FOEG), and higher oxidative stress (T-SH: +53.0% vs. CG and+60.2% vs. FOEG) compared with the other two groups (P<0.05). Serum triglyceride (TG) concentration decreased in FOG and FOEG compared with CG, on day 60 (P<0.01). Conclusion: We concluded that total antioxidant activitydecreased and oxidative stress increased in seminal plasma of dogs after FO supplementation for 60 days.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/118871
url http://sedici.unlp.edu.ar/handle/10915/118871
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2008-076X
info:eu-repo/semantics/altIdentifier/issn/2008-077
info:eu-repo/semantics/altIdentifier/doi/10.22074/IJFS.2021.6226
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/3.0/
Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/3.0/
Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0)
dc.format.none.fl_str_mv application/pdf
15-19
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instname:Universidad Nacional de La Plata
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instname_str Universidad Nacional de La Plata
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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