Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs

Autores
Risso, Analia Lorena; Pellegrino, Francisco Javier; Corrada, Yanina Alejandra; Marmunti, Mónica Edith; Gavazza, Mariana Beatriz; Palacios, Alejandro; Relling, Alejandro Enrique
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective was to evaluate the effects of dietary fish oil (FO) and vitamin E (VE) supplementation on sperm sensitivity to lipid peroxidation (LP) in dogs. Using an incomplete replicate 3 × 3 Latin square design, five dogs were allocated into three groups. One of the squares was incomplete and had two dogs that were used with three treatments. The dogs were assigned to three different treatments, fed a control diet of balanced commercial food (controlgroup; CG), control diet supplemented with 54 mg FO/kg body weight 0·75 per day (FO group; FG) and FO plus 400 mg VE per day (FO and VE group; FEG) for 60 d. Semen samples were collected on days 0 and 60 and divided into two halves, peroxidised and control, with or without ascorbate?Fe2+, respectively. LP was measured in both halves by chemiluminescence as counts per min/mg protein. Fatty acid profile was determined by GC. Data were analysed using the mixed procedure (SAS). On day 0, LP increased in all groups in the peroxidised samples (P < 0·05). However, on day 60 LP decreased in peroxidised samples of both the FG and FEG (P < 0·05), but there were no differences between the FG and FEG (P > 0·1). Additionally, on day 60 total n-3 was higher in the FG and FEG compared with the CG (P < 0·05). Supplementation with FO alone or together with VE decreased LP in peroxidised samples. These results could indicate a protective effect of n-3 on sperm. More studies are needed to understand the mechanism whereby FO and/or FO plus VE decrease LP in dogs? sperm.
Fil: Risso, Analia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Pellegrino, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Corrada, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Marmunti, Mónica Edith. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Gavazza, Mariana Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Palacios, Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Relling, Alejandro Enrique. Ohio State University; Estados Unidos
Materia
Dog Nutrition
Fish Oil
Vitamin E
Lipid Peroxidation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48500
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/48500
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effect of fish oil and vitamin E on sperm lipid peroxidation in dogsRisso, Analia LorenaPellegrino, Francisco JavierCorrada, Yanina AlejandraMarmunti, Mónica EdithGavazza, Mariana BeatrizPalacios, AlejandroRelling, Alejandro EnriqueDog NutritionFish OilVitamin ELipid Peroxidationhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The objective was to evaluate the effects of dietary fish oil (FO) and vitamin E (VE) supplementation on sperm sensitivity to lipid peroxidation (LP) in dogs. Using an incomplete replicate 3 × 3 Latin square design, five dogs were allocated into three groups. One of the squares was incomplete and had two dogs that were used with three treatments. The dogs were assigned to three different treatments, fed a control diet of balanced commercial food (controlgroup; CG), control diet supplemented with 54 mg FO/kg body weight 0·75 per day (FO group; FG) and FO plus 400 mg VE per day (FO and VE group; FEG) for 60 d. Semen samples were collected on days 0 and 60 and divided into two halves, peroxidised and control, with or without ascorbate?Fe2+, respectively. LP was measured in both halves by chemiluminescence as counts per min/mg protein. Fatty acid profile was determined by GC. Data were analysed using the mixed procedure (SAS). On day 0, LP increased in all groups in the peroxidised samples (P < 0·05). However, on day 60 LP decreased in peroxidised samples of both the FG and FEG (P < 0·05), but there were no differences between the FG and FEG (P > 0·1). Additionally, on day 60 total n-3 was higher in the FG and FEG compared with the CG (P < 0·05). Supplementation with FO alone or together with VE decreased LP in peroxidised samples. These results could indicate a protective effect of n-3 on sperm. More studies are needed to understand the mechanism whereby FO and/or FO plus VE decrease LP in dogs? sperm.Fil: Risso, Analia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Pellegrino, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Corrada, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Marmunti, Mónica Edith. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Gavazza, Mariana Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Palacios, Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Relling, Alejandro Enrique. Ohio State University; Estados UnidosCambridge University Press2017-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48500Risso, Analia Lorena; Pellegrino, Francisco Javier; Corrada, Yanina Alejandra; Marmunti, Mónica Edith; Gavazza, Mariana Beatriz; et al.; Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs; Cambridge University Press; Journal of Nutritional Science; 6; 48; 9-2017; 1-42048-6790CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1017/jns.2017.29info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/journal-of-nutritional-science/article/effect-of-fish-oil-and-vitamin-e-on-sperm-lipid-peroxidation-in-dogs/3B9889CBA7EB23B974F8F0B984D8643Binfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:08Zoai:ri.conicet.gov.ar:11336/48500instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:08.741CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs
title Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs
spellingShingle Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs
Risso, Analia Lorena
Dog Nutrition
Fish Oil
Vitamin E
Lipid Peroxidation
title_short Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs
title_full Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs
title_fullStr Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs
title_full_unstemmed Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs
title_sort Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs
dc.creator.none.fl_str_mv Risso, Analia Lorena
Pellegrino, Francisco Javier
Corrada, Yanina Alejandra
Marmunti, Mónica Edith
Gavazza, Mariana Beatriz
Palacios, Alejandro
Relling, Alejandro Enrique
author Risso, Analia Lorena
author_facet Risso, Analia Lorena
Pellegrino, Francisco Javier
Corrada, Yanina Alejandra
Marmunti, Mónica Edith
Gavazza, Mariana Beatriz
Palacios, Alejandro
Relling, Alejandro Enrique
author_role author
author2 Pellegrino, Francisco Javier
Corrada, Yanina Alejandra
Marmunti, Mónica Edith
Gavazza, Mariana Beatriz
Palacios, Alejandro
Relling, Alejandro Enrique
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Dog Nutrition
Fish Oil
Vitamin E
Lipid Peroxidation
topic Dog Nutrition
Fish Oil
Vitamin E
Lipid Peroxidation
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The objective was to evaluate the effects of dietary fish oil (FO) and vitamin E (VE) supplementation on sperm sensitivity to lipid peroxidation (LP) in dogs. Using an incomplete replicate 3 × 3 Latin square design, five dogs were allocated into three groups. One of the squares was incomplete and had two dogs that were used with three treatments. The dogs were assigned to three different treatments, fed a control diet of balanced commercial food (controlgroup; CG), control diet supplemented with 54 mg FO/kg body weight 0·75 per day (FO group; FG) and FO plus 400 mg VE per day (FO and VE group; FEG) for 60 d. Semen samples were collected on days 0 and 60 and divided into two halves, peroxidised and control, with or without ascorbate?Fe2+, respectively. LP was measured in both halves by chemiluminescence as counts per min/mg protein. Fatty acid profile was determined by GC. Data were analysed using the mixed procedure (SAS). On day 0, LP increased in all groups in the peroxidised samples (P < 0·05). However, on day 60 LP decreased in peroxidised samples of both the FG and FEG (P < 0·05), but there were no differences between the FG and FEG (P > 0·1). Additionally, on day 60 total n-3 was higher in the FG and FEG compared with the CG (P < 0·05). Supplementation with FO alone or together with VE decreased LP in peroxidised samples. These results could indicate a protective effect of n-3 on sperm. More studies are needed to understand the mechanism whereby FO and/or FO plus VE decrease LP in dogs? sperm.
Fil: Risso, Analia Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Pellegrino, Francisco Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Corrada, Yanina Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Marmunti, Mónica Edith. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Gavazza, Mariana Beatriz. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Palacios, Alejandro. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Relling, Alejandro Enrique. Ohio State University; Estados Unidos
description The objective was to evaluate the effects of dietary fish oil (FO) and vitamin E (VE) supplementation on sperm sensitivity to lipid peroxidation (LP) in dogs. Using an incomplete replicate 3 × 3 Latin square design, five dogs were allocated into three groups. One of the squares was incomplete and had two dogs that were used with three treatments. The dogs were assigned to three different treatments, fed a control diet of balanced commercial food (controlgroup; CG), control diet supplemented with 54 mg FO/kg body weight 0·75 per day (FO group; FG) and FO plus 400 mg VE per day (FO and VE group; FEG) for 60 d. Semen samples were collected on days 0 and 60 and divided into two halves, peroxidised and control, with or without ascorbate?Fe2+, respectively. LP was measured in both halves by chemiluminescence as counts per min/mg protein. Fatty acid profile was determined by GC. Data were analysed using the mixed procedure (SAS). On day 0, LP increased in all groups in the peroxidised samples (P < 0·05). However, on day 60 LP decreased in peroxidised samples of both the FG and FEG (P < 0·05), but there were no differences between the FG and FEG (P > 0·1). Additionally, on day 60 total n-3 was higher in the FG and FEG compared with the CG (P < 0·05). Supplementation with FO alone or together with VE decreased LP in peroxidised samples. These results could indicate a protective effect of n-3 on sperm. More studies are needed to understand the mechanism whereby FO and/or FO plus VE decrease LP in dogs? sperm.
publishDate 2017
dc.date.none.fl_str_mv 2017-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48500
Risso, Analia Lorena; Pellegrino, Francisco Javier; Corrada, Yanina Alejandra; Marmunti, Mónica Edith; Gavazza, Mariana Beatriz; et al.; Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs; Cambridge University Press; Journal of Nutritional Science; 6; 48; 9-2017; 1-4
2048-6790
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48500
identifier_str_mv Risso, Analia Lorena; Pellegrino, Francisco Javier; Corrada, Yanina Alejandra; Marmunti, Mónica Edith; Gavazza, Mariana Beatriz; et al.; Effect of fish oil and vitamin E on sperm lipid peroxidation in dogs; Cambridge University Press; Journal of Nutritional Science; 6; 48; 9-2017; 1-4
2048-6790
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1017/jns.2017.29
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/journal-of-nutritional-science/article/effect-of-fish-oil-and-vitamin-e-on-sperm-lipid-peroxidation-in-dogs/3B9889CBA7EB23B974F8F0B984D8643B
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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