Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs

Autores
Pellegrino, Francisco Javier; Risso, Analia Lorena; Corrada, Yanina Alejandra; Gambaro, Rocío Celeste; Seoane, Analia Isabel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Fish oil (FO) supplementation as a source of omega 3 fatty acids is associated with beneficial effects on health. However, high unsaturated fatty acid content in thediet could result in increased lipid peroxidation and damage to proteins, lipids and DNA.We evaluated the effect of dietary FO supplementation on DNA damage in peripheralblood lymphocytes of dogs. Additionally, we determined the effect of FO supplementation on lipid peroxidation and lipid profile of these dogs. Methods: Healthy male dogs (n = 9) were randomly assigned to one of two diets during90 days: control (CG, n = 4), based on a commercial food, and FO (FOG, n = 5), thesame food supplemented with 1000 mg FO. Blood samples were collected on days −1,30, 60 and 90. DNA damage was assessed with the comet assay, and the damage indexwas obtained. Malondialdehyde (MDA) levels were determined as an indicator of lipidperoxidation. Lipid profile determination included serum triglyceride, cholesterol, lowdensity lipoprotein and high-density lipoprotein levels (HDL). Results: Damage index values (arbitrary units) were lower in FOG on day 30 (CG,13.7 ± 2.5; FOG, 6.5 ± 2.5), 60 (CG, 14.7 ± 2.5; FOG, 3.5 ± 2.5) and 90 (CG, 15.5 ±2.5; FOG, 3.0 ± 2.5) compared with CG (treatment × time interaction, p < 0.01). Serum MDA and HDL concentrations were lower in FOG compared with CG on day 60 and90 (treatment × time interaction, p < 0.05). Conclusion: These findings suggest that dietary FO supplementation did not induceDNA damage in peripheral blood lymphocytes of healthy dogs, but rather reduced it.
Fil: Pellegrino, Francisco Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Risso, Analia Lorena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Corrada, Yanina Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gambaro, Rocío Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Materia
DOG
FISH OIL SUPPLEMENTATION
DNA DAMAGE
LYMPHOCYTES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/156534
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/156534
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogsPellegrino, Francisco JavierRisso, Analia LorenaCorrada, Yanina AlejandraGambaro, Rocío CelesteSeoane, Analia IsabelDOGFISH OIL SUPPLEMENTATIONDNA DAMAGELYMPHOCYTEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Background: Fish oil (FO) supplementation as a source of omega 3 fatty acids is associated with beneficial effects on health. However, high unsaturated fatty acid content in thediet could result in increased lipid peroxidation and damage to proteins, lipids and DNA.We evaluated the effect of dietary FO supplementation on DNA damage in peripheralblood lymphocytes of dogs. Additionally, we determined the effect of FO supplementation on lipid peroxidation and lipid profile of these dogs. Methods: Healthy male dogs (n = 9) were randomly assigned to one of two diets during90 days: control (CG, n = 4), based on a commercial food, and FO (FOG, n = 5), thesame food supplemented with 1000 mg FO. Blood samples were collected on days −1,30, 60 and 90. DNA damage was assessed with the comet assay, and the damage indexwas obtained. Malondialdehyde (MDA) levels were determined as an indicator of lipidperoxidation. Lipid profile determination included serum triglyceride, cholesterol, lowdensity lipoprotein and high-density lipoprotein levels (HDL). Results: Damage index values (arbitrary units) were lower in FOG on day 30 (CG,13.7 ± 2.5; FOG, 6.5 ± 2.5), 60 (CG, 14.7 ± 2.5; FOG, 3.5 ± 2.5) and 90 (CG, 15.5 ±2.5; FOG, 3.0 ± 2.5) compared with CG (treatment × time interaction, p < 0.01). Serum MDA and HDL concentrations were lower in FOG compared with CG on day 60 and90 (treatment × time interaction, p < 0.05). Conclusion: These findings suggest that dietary FO supplementation did not induceDNA damage in peripheral blood lymphocytes of healthy dogs, but rather reduced it.Fil: Pellegrino, Francisco Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Risso, Analia Lorena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Corrada, Yanina Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; ArgentinaFil: Gambaro, Rocío Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaWiley2021-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/156534Pellegrino, Francisco Javier; Risso, Analia Lorena; Corrada, Yanina Alejandra; Gambaro, Rocío Celeste; Seoane, Analia Isabel; Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs; Wiley; Veterinary Record Open; 8; 1; 6-2021; 1-52052-6113CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/vro2.12info:eu-repo/semantics/altIdentifier/doi/10.1002/vro2.12info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:53:02Zoai:ri.conicet.gov.ar:11336/156534instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:53:02.66CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
spellingShingle Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
Pellegrino, Francisco Javier
DOG
FISH OIL SUPPLEMENTATION
DNA DAMAGE
LYMPHOCYTES
title_short Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title_full Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title_fullStr Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title_full_unstemmed Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
title_sort Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs
dc.creator.none.fl_str_mv Pellegrino, Francisco Javier
Risso, Analia Lorena
Corrada, Yanina Alejandra
Gambaro, Rocío Celeste
Seoane, Analia Isabel
author Pellegrino, Francisco Javier
author_facet Pellegrino, Francisco Javier
Risso, Analia Lorena
Corrada, Yanina Alejandra
Gambaro, Rocío Celeste
Seoane, Analia Isabel
author_role author
author2 Risso, Analia Lorena
Corrada, Yanina Alejandra
Gambaro, Rocío Celeste
Seoane, Analia Isabel
author2_role author
author
author
author
dc.subject.none.fl_str_mv DOG
FISH OIL SUPPLEMENTATION
DNA DAMAGE
LYMPHOCYTES
topic DOG
FISH OIL SUPPLEMENTATION
DNA DAMAGE
LYMPHOCYTES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Background: Fish oil (FO) supplementation as a source of omega 3 fatty acids is associated with beneficial effects on health. However, high unsaturated fatty acid content in thediet could result in increased lipid peroxidation and damage to proteins, lipids and DNA.We evaluated the effect of dietary FO supplementation on DNA damage in peripheralblood lymphocytes of dogs. Additionally, we determined the effect of FO supplementation on lipid peroxidation and lipid profile of these dogs. Methods: Healthy male dogs (n = 9) were randomly assigned to one of two diets during90 days: control (CG, n = 4), based on a commercial food, and FO (FOG, n = 5), thesame food supplemented with 1000 mg FO. Blood samples were collected on days −1,30, 60 and 90. DNA damage was assessed with the comet assay, and the damage indexwas obtained. Malondialdehyde (MDA) levels were determined as an indicator of lipidperoxidation. Lipid profile determination included serum triglyceride, cholesterol, lowdensity lipoprotein and high-density lipoprotein levels (HDL). Results: Damage index values (arbitrary units) were lower in FOG on day 30 (CG,13.7 ± 2.5; FOG, 6.5 ± 2.5), 60 (CG, 14.7 ± 2.5; FOG, 3.5 ± 2.5) and 90 (CG, 15.5 ±2.5; FOG, 3.0 ± 2.5) compared with CG (treatment × time interaction, p < 0.01). Serum MDA and HDL concentrations were lower in FOG compared with CG on day 60 and90 (treatment × time interaction, p < 0.05). Conclusion: These findings suggest that dietary FO supplementation did not induceDNA damage in peripheral blood lymphocytes of healthy dogs, but rather reduced it.
Fil: Pellegrino, Francisco Javier. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Risso, Analia Lorena. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Corrada, Yanina Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata; Argentina
Fil: Gambaro, Rocío Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Seoane, Analia Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
description Background: Fish oil (FO) supplementation as a source of omega 3 fatty acids is associated with beneficial effects on health. However, high unsaturated fatty acid content in thediet could result in increased lipid peroxidation and damage to proteins, lipids and DNA.We evaluated the effect of dietary FO supplementation on DNA damage in peripheralblood lymphocytes of dogs. Additionally, we determined the effect of FO supplementation on lipid peroxidation and lipid profile of these dogs. Methods: Healthy male dogs (n = 9) were randomly assigned to one of two diets during90 days: control (CG, n = 4), based on a commercial food, and FO (FOG, n = 5), thesame food supplemented with 1000 mg FO. Blood samples were collected on days −1,30, 60 and 90. DNA damage was assessed with the comet assay, and the damage indexwas obtained. Malondialdehyde (MDA) levels were determined as an indicator of lipidperoxidation. Lipid profile determination included serum triglyceride, cholesterol, lowdensity lipoprotein and high-density lipoprotein levels (HDL). Results: Damage index values (arbitrary units) were lower in FOG on day 30 (CG,13.7 ± 2.5; FOG, 6.5 ± 2.5), 60 (CG, 14.7 ± 2.5; FOG, 3.5 ± 2.5) and 90 (CG, 15.5 ±2.5; FOG, 3.0 ± 2.5) compared with CG (treatment × time interaction, p < 0.01). Serum MDA and HDL concentrations were lower in FOG compared with CG on day 60 and90 (treatment × time interaction, p < 0.05). Conclusion: These findings suggest that dietary FO supplementation did not induceDNA damage in peripheral blood lymphocytes of healthy dogs, but rather reduced it.
publishDate 2021
dc.date.none.fl_str_mv 2021-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/156534
Pellegrino, Francisco Javier; Risso, Analia Lorena; Corrada, Yanina Alejandra; Gambaro, Rocío Celeste; Seoane, Analia Isabel; Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs; Wiley; Veterinary Record Open; 8; 1; 6-2021; 1-5
2052-6113
CONICET Digital
CONICET
url http://hdl.handle.net/11336/156534
identifier_str_mv Pellegrino, Francisco Javier; Risso, Analia Lorena; Corrada, Yanina Alejandra; Gambaro, Rocío Celeste; Seoane, Analia Isabel; Influence of dietary fish oil supplementation on DNA damage in peripheral blood lymphocytes of nine healthy dogs; Wiley; Veterinary Record Open; 8; 1; 6-2021; 1-5
2052-6113
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/vro2.12
info:eu-repo/semantics/altIdentifier/doi/10.1002/vro2.12
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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