Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>

Autores
Herman, Cristian; Maguna, Fabiana P.; Garro, Oscar Alfredo; Castro, Eduardo Alberto
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to determine the influence of pH, sodium chloride concentration and the combination of different temperatures and periods of exposure on the antimicrobial activity against Lactobacillus fructivorans. Turbidity of the different system conditions was monitored in order to establish the microorganism growth. The effect of temperatures ranging from 60 to 120ºC during periods of 10 to 60 minutes was evaluated. Nisin activity was only changed by temperatures ranging from 100 to 120ºC, in several exposure times. A pH range between 3.5 and 7 was tested. The highest inhibitory effect was recorded for neutral pH and lightly acidic conditions. Sodium chloride concentrations studied were 1 and 2 % w/v. The latter value helped to enhance the inhibitory effect of the bacteriocin. These results show the influence that the studied factors exert on nisin activity against L. fructivorans and suggest that they have to be taken into account when nisin is to be used as a biopreservative in food.
El presente trabajo tuvo como objetivo determinar la influencia del pH, concentración de cloruro de sodio y la combinación de diferentes temperaturas y períodos de exposición sobre la actividad antimicrobiana de nisina s obre una bacteria alterante Lactobacillus fructivorans. El crecimiento del microorganismo bajo diferentes condiciones fue determinado por la presencia de turbidez. Se evaluó el efecto de la temperatura en un rango de 60 a 120ºC durante períodos de 10 a 60 minutos. La actividad de la nisina fue afectada solamente por temperaturas de 100 y 120ºC, a cualquier tiempo de exposición considerado. Los ensayos se realizaron con un rango de pH entre 3,5 y 7. El mayor efecto inhibitorio fue a pH neutro y levemente ácido. Las concentraciones de cloruro de sodio fueron de 1 y 2% p/v. Sólo a la mayor concentración se ve un incremento en la actividad inhibitoria de la bacteriocina. Estos resultados muestran la influencia que los factores estudiados ejercen sobre la actividad de la bacteriocina frente a L. fructivorans e indican que tales factores deben ser tenidos en cuenta cuando la nisina sea usada en la preservación de alimentos.
Centro de Investigación y Desarrollo en Ciencias Aplicadas
Materia
Química
Nisin
Lactobacillus fructivorans
Temperature
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/100759

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network_name_str SEDICI (UNLP)
spelling Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>Herman, CristianMaguna, Fabiana P.Garro, Oscar AlfredoCastro, Eduardo AlbertoQuímicaNisinLactobacillus fructivoransTemperatureThe aim of this work was to determine the influence of pH, sodium chloride concentration and the combination of different temperatures and periods of exposure on the antimicrobial activity against Lactobacillus fructivorans. Turbidity of the different system conditions was monitored in order to establish the microorganism growth. The effect of temperatures ranging from 60 to 120ºC during periods of 10 to 60 minutes was evaluated. Nisin activity was only changed by temperatures ranging from 100 to 120ºC, in several exposure times. A pH range between 3.5 and 7 was tested. The highest inhibitory effect was recorded for neutral pH and lightly acidic conditions. Sodium chloride concentrations studied were 1 and 2 % w/v. The latter value helped to enhance the inhibitory effect of the bacteriocin. These results show the influence that the studied factors exert on nisin activity against L. fructivorans and suggest that they have to be taken into account when nisin is to be used as a biopreservative in food.El presente trabajo tuvo como objetivo determinar la influencia del pH, concentración de cloruro de sodio y la combinación de diferentes temperaturas y períodos de exposición sobre la actividad antimicrobiana de nisina s obre una bacteria alterante Lactobacillus fructivorans. El crecimiento del microorganismo bajo diferentes condiciones fue determinado por la presencia de turbidez. Se evaluó el efecto de la temperatura en un rango de 60 a 120ºC durante períodos de 10 a 60 minutos. La actividad de la nisina fue afectada solamente por temperaturas de 100 y 120ºC, a cualquier tiempo de exposición considerado. Los ensayos se realizaron con un rango de pH entre 3,5 y 7. El mayor efecto inhibitorio fue a pH neutro y levemente ácido. Las concentraciones de cloruro de sodio fueron de 1 y 2% p/v. Sólo a la mayor concentración se ve un incremento en la actividad inhibitoria de la bacteriocina. Estos resultados muestran la influencia que los factores estudiados ejercen sobre la actividad de la bacteriocina frente a L. fructivorans e indican que tales factores deben ser tenidos en cuenta cuando la nisina sea usada en la preservación de alimentos.Centro de Investigación y Desarrollo en Ciencias Aplicadas2009-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf11-18http://sedici.unlp.edu.ar/handle/10915/100759enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/58598info:eu-repo/semantics/altIdentifier/url/https://www.aqa.org.ar/images/anales/pdf9702/9702art2.pdfinfo:eu-repo/semantics/altIdentifier/issn/0365-0375info:eu-repo/semantics/altIdentifier/hdl/11336/58598info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:01:55Zoai:sedici.unlp.edu.ar:10915/100759Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:01:56.273SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>
title Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>
spellingShingle Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>
Herman, Cristian
Química
Nisin
Lactobacillus fructivorans
Temperature
title_short Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>
title_full Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>
title_fullStr Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>
title_full_unstemmed Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>
title_sort Effect of temperature, pH and NaCl on nisin activity against <i>Lactobacillus fructivorans</i>
dc.creator.none.fl_str_mv Herman, Cristian
Maguna, Fabiana P.
Garro, Oscar Alfredo
Castro, Eduardo Alberto
author Herman, Cristian
author_facet Herman, Cristian
Maguna, Fabiana P.
Garro, Oscar Alfredo
Castro, Eduardo Alberto
author_role author
author2 Maguna, Fabiana P.
Garro, Oscar Alfredo
Castro, Eduardo Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Química
Nisin
Lactobacillus fructivorans
Temperature
topic Química
Nisin
Lactobacillus fructivorans
Temperature
dc.description.none.fl_txt_mv The aim of this work was to determine the influence of pH, sodium chloride concentration and the combination of different temperatures and periods of exposure on the antimicrobial activity against Lactobacillus fructivorans. Turbidity of the different system conditions was monitored in order to establish the microorganism growth. The effect of temperatures ranging from 60 to 120ºC during periods of 10 to 60 minutes was evaluated. Nisin activity was only changed by temperatures ranging from 100 to 120ºC, in several exposure times. A pH range between 3.5 and 7 was tested. The highest inhibitory effect was recorded for neutral pH and lightly acidic conditions. Sodium chloride concentrations studied were 1 and 2 % w/v. The latter value helped to enhance the inhibitory effect of the bacteriocin. These results show the influence that the studied factors exert on nisin activity against L. fructivorans and suggest that they have to be taken into account when nisin is to be used as a biopreservative in food.
El presente trabajo tuvo como objetivo determinar la influencia del pH, concentración de cloruro de sodio y la combinación de diferentes temperaturas y períodos de exposición sobre la actividad antimicrobiana de nisina s obre una bacteria alterante Lactobacillus fructivorans. El crecimiento del microorganismo bajo diferentes condiciones fue determinado por la presencia de turbidez. Se evaluó el efecto de la temperatura en un rango de 60 a 120ºC durante períodos de 10 a 60 minutos. La actividad de la nisina fue afectada solamente por temperaturas de 100 y 120ºC, a cualquier tiempo de exposición considerado. Los ensayos se realizaron con un rango de pH entre 3,5 y 7. El mayor efecto inhibitorio fue a pH neutro y levemente ácido. Las concentraciones de cloruro de sodio fueron de 1 y 2% p/v. Sólo a la mayor concentración se ve un incremento en la actividad inhibitoria de la bacteriocina. Estos resultados muestran la influencia que los factores estudiados ejercen sobre la actividad de la bacteriocina frente a L. fructivorans e indican que tales factores deben ser tenidos en cuenta cuando la nisina sea usada en la preservación de alimentos.
Centro de Investigación y Desarrollo en Ciencias Aplicadas
description The aim of this work was to determine the influence of pH, sodium chloride concentration and the combination of different temperatures and periods of exposure on the antimicrobial activity against Lactobacillus fructivorans. Turbidity of the different system conditions was monitored in order to establish the microorganism growth. The effect of temperatures ranging from 60 to 120ºC during periods of 10 to 60 minutes was evaluated. Nisin activity was only changed by temperatures ranging from 100 to 120ºC, in several exposure times. A pH range between 3.5 and 7 was tested. The highest inhibitory effect was recorded for neutral pH and lightly acidic conditions. Sodium chloride concentrations studied were 1 and 2 % w/v. The latter value helped to enhance the inhibitory effect of the bacteriocin. These results show the influence that the studied factors exert on nisin activity against L. fructivorans and suggest that they have to be taken into account when nisin is to be used as a biopreservative in food.
publishDate 2009
dc.date.none.fl_str_mv 2009-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/100759
url http://sedici.unlp.edu.ar/handle/10915/100759
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/issn/0365-0375
info:eu-repo/semantics/altIdentifier/hdl/11336/58598
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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