The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa

Autores
Palacios, Alejandro; Catalá, Ángel
Año de publicación
1991
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The palmitic acid binding capacity of cytosolic proteins in three preparations obtained by differential scraping of bovine intestinal mucosa were compared. The data indicated that the palmitic acid binding activities depended on the position that the cells occupied along the crypt-villus axis, as shown from the level of alkaline phosphatase activity. Proteins with palmitate binding properties in the high- and low-molecular-weight cytosolic proteins in the villus zone bound 1.24±0.41 and 1.54±0.16 pmol palminate/μg protein respectively. The binding decreased to 0.50±0.25 and 1.10±0.23 pmol palmitate/μg for the proteins in the crypt zone. Ammonium sulphate fractionation and gel filtration chromatography indicated that the low-molecular-weight cytosolic proteins obtained from light mucosal scrapings contained the highest palmitate binding activity. These results suggest that the cytosolic proteins located in the villus zone may play a role in the absorption of fatty acids.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Bioquímica
alkaline phosphatase
cattle
intestine
mucosa
palmitic acid
protein
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/138100
Acceder
id SEDICI_bf215d38f425dffdd282b3cac3e7c346
oai_identifier_str oai:sedici.unlp.edu.ar:10915/138100
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosaPalacios, AlejandroCatalá, ÁngelCiencias VeterinariasBioquímicaalkaline phosphatasecattleintestinemucosapalmitic acidproteinThe palmitic acid binding capacity of cytosolic proteins in three preparations obtained by differential scraping of bovine intestinal mucosa were compared. The data indicated that the palmitic acid binding activities depended on the position that the cells occupied along the crypt-villus axis, as shown from the level of alkaline phosphatase activity. Proteins with palmitate binding properties in the high- and low-molecular-weight cytosolic proteins in the villus zone bound 1.24±0.41 and 1.54±0.16 pmol palminate/μg protein respectively. The binding decreased to 0.50±0.25 and 1.10±0.23 pmol palmitate/μg for the proteins in the crypt zone. Ammonium sulphate fractionation and gel filtration chromatography indicated that the low-molecular-weight cytosolic proteins obtained from light mucosal scrapings contained the highest palmitate binding activity. These results suggest that the cytosolic proteins located in the villus zone may play a role in the absorption of fatty acids.Facultad de Ciencias Veterinarias1991info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf437-442http://sedici.unlp.edu.ar/handle/10915/138100enginfo:eu-repo/semantics/altIdentifier/issn/0165-7380info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00346540info:eu-repo/semantics/altIdentifier/pmid/1803723info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:03Zoai:sedici.unlp.edu.ar:10915/138100Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:03.419SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa
title The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa
spellingShingle The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa
Palacios, Alejandro
Ciencias Veterinarias
Bioquímica
alkaline phosphatase
cattle
intestine
mucosa
palmitic acid
protein
title_short The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa
title_full The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa
title_fullStr The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa
title_full_unstemmed The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa
title_sort The palmitic acid binding properties of cytosolic proteins located in the villus and crypt zones of bovine intestinal mucosa
dc.creator.none.fl_str_mv Palacios, Alejandro
Catalá, Ángel
author Palacios, Alejandro
author_facet Palacios, Alejandro
Catalá, Ángel
author_role author
author2 Catalá, Ángel
author2_role author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Bioquímica
alkaline phosphatase
cattle
intestine
mucosa
palmitic acid
protein
topic Ciencias Veterinarias
Bioquímica
alkaline phosphatase
cattle
intestine
mucosa
palmitic acid
protein
dc.description.none.fl_txt_mv The palmitic acid binding capacity of cytosolic proteins in three preparations obtained by differential scraping of bovine intestinal mucosa were compared. The data indicated that the palmitic acid binding activities depended on the position that the cells occupied along the crypt-villus axis, as shown from the level of alkaline phosphatase activity. Proteins with palmitate binding properties in the high- and low-molecular-weight cytosolic proteins in the villus zone bound 1.24±0.41 and 1.54±0.16 pmol palminate/μg protein respectively. The binding decreased to 0.50±0.25 and 1.10±0.23 pmol palmitate/μg for the proteins in the crypt zone. Ammonium sulphate fractionation and gel filtration chromatography indicated that the low-molecular-weight cytosolic proteins obtained from light mucosal scrapings contained the highest palmitate binding activity. These results suggest that the cytosolic proteins located in the villus zone may play a role in the absorption of fatty acids.
Facultad de Ciencias Veterinarias
description The palmitic acid binding capacity of cytosolic proteins in three preparations obtained by differential scraping of bovine intestinal mucosa were compared. The data indicated that the palmitic acid binding activities depended on the position that the cells occupied along the crypt-villus axis, as shown from the level of alkaline phosphatase activity. Proteins with palmitate binding properties in the high- and low-molecular-weight cytosolic proteins in the villus zone bound 1.24±0.41 and 1.54±0.16 pmol palminate/μg protein respectively. The binding decreased to 0.50±0.25 and 1.10±0.23 pmol palmitate/μg for the proteins in the crypt zone. Ammonium sulphate fractionation and gel filtration chromatography indicated that the low-molecular-weight cytosolic proteins obtained from light mucosal scrapings contained the highest palmitate binding activity. These results suggest that the cytosolic proteins located in the villus zone may play a role in the absorption of fatty acids.
publishDate 1991
dc.date.none.fl_str_mv 1991
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/138100
url http://sedici.unlp.edu.ar/handle/10915/138100
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0165-7380
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00346540
info:eu-repo/semantics/altIdentifier/pmid/1803723
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
437-442
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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