The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine

Autores
Muglia, Cecilia Isabel; Mercer, Natalia; Toscano, M. A.; Schattner, M.; Pozner, Raúl Ernesto; Cerliani, J. P.; Papa Gobbi, Rodrigo; Rabinovich, G. A.; Docena, Guillermo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function.
Facultad de Ciencias Exactas
Materia
Ciencias Exactas
Apoptosis
Enterocytes
Galectin-1
Mucosa
Small bowel
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83984

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network_name_str SEDICI (UNLP)
spelling The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestineMuglia, Cecilia IsabelMercer, NataliaToscano, M. A.Schattner, M.Pozner, Raúl ErnestoCerliani, J. P.Papa Gobbi, RodrigoRabinovich, G. A.Docena, GuillermoCiencias ExactasApoptosisEnterocytesGalectin-1MucosaSmall bowelAbstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function.Facultad de Ciencias Exactas2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/83984enginfo:eu-repo/semantics/altIdentifier/issn/2041-4889info:eu-repo/semantics/altIdentifier/doi/10.1038/cddis.2011.44info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:10Zoai:sedici.unlp.edu.ar:10915/83984Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:10.283SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
spellingShingle The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
Muglia, Cecilia Isabel
Ciencias Exactas
Apoptosis
Enterocytes
Galectin-1
Mucosa
Small bowel
title_short The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title_full The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title_fullStr The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title_full_unstemmed The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
title_sort The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
dc.creator.none.fl_str_mv Muglia, Cecilia Isabel
Mercer, Natalia
Toscano, M. A.
Schattner, M.
Pozner, Raúl Ernesto
Cerliani, J. P.
Papa Gobbi, Rodrigo
Rabinovich, G. A.
Docena, Guillermo
author Muglia, Cecilia Isabel
author_facet Muglia, Cecilia Isabel
Mercer, Natalia
Toscano, M. A.
Schattner, M.
Pozner, Raúl Ernesto
Cerliani, J. P.
Papa Gobbi, Rodrigo
Rabinovich, G. A.
Docena, Guillermo
author_role author
author2 Mercer, Natalia
Toscano, M. A.
Schattner, M.
Pozner, Raúl Ernesto
Cerliani, J. P.
Papa Gobbi, Rodrigo
Rabinovich, G. A.
Docena, Guillermo
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Apoptosis
Enterocytes
Galectin-1
Mucosa
Small bowel
topic Ciencias Exactas
Apoptosis
Enterocytes
Galectin-1
Mucosa
Small bowel
dc.description.none.fl_txt_mv Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function.
Facultad de Ciencias Exactas
description Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83984
url http://sedici.unlp.edu.ar/handle/10915/83984
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/2041-4889
info:eu-repo/semantics/altIdentifier/doi/10.1038/cddis.2011.44
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
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