The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine
- Autores
- Muglia, Cecilia Isabel; Mercer, Natalia; Toscano, M. A.; Schattner, M.; Pozner, Raúl Ernesto; Cerliani, J. P.; Papa Gobbi, Rodrigo; Rabinovich, G. A.; Docena, Guillermo
- Año de publicación
- 2011
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function.
Facultad de Ciencias Exactas - Materia
-
Ciencias Exactas
Apoptosis
Enterocytes
Galectin-1
Mucosa
Small bowel - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/83984
Ver los metadatos del registro completo
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The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestineMuglia, Cecilia IsabelMercer, NataliaToscano, M. A.Schattner, M.Pozner, Raúl ErnestoCerliani, J. P.Papa Gobbi, RodrigoRabinovich, G. A.Docena, GuillermoCiencias ExactasApoptosisEnterocytesGalectin-1MucosaSmall bowelAbstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function.Facultad de Ciencias Exactas2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/83984enginfo:eu-repo/semantics/altIdentifier/issn/2041-4889info:eu-repo/semantics/altIdentifier/doi/10.1038/cddis.2011.44info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:16:10Zoai:sedici.unlp.edu.ar:10915/83984Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:16:10.283SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
title |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
spellingShingle |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine Muglia, Cecilia Isabel Ciencias Exactas Apoptosis Enterocytes Galectin-1 Mucosa Small bowel |
title_short |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
title_full |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
title_fullStr |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
title_full_unstemmed |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
title_sort |
The glycan-binding protein galectin-1 controls survival of epithelial cells along the crypt-villus axis of small intestine |
dc.creator.none.fl_str_mv |
Muglia, Cecilia Isabel Mercer, Natalia Toscano, M. A. Schattner, M. Pozner, Raúl Ernesto Cerliani, J. P. Papa Gobbi, Rodrigo Rabinovich, G. A. Docena, Guillermo |
author |
Muglia, Cecilia Isabel |
author_facet |
Muglia, Cecilia Isabel Mercer, Natalia Toscano, M. A. Schattner, M. Pozner, Raúl Ernesto Cerliani, J. P. Papa Gobbi, Rodrigo Rabinovich, G. A. Docena, Guillermo |
author_role |
author |
author2 |
Mercer, Natalia Toscano, M. A. Schattner, M. Pozner, Raúl Ernesto Cerliani, J. P. Papa Gobbi, Rodrigo Rabinovich, G. A. Docena, Guillermo |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Exactas Apoptosis Enterocytes Galectin-1 Mucosa Small bowel |
topic |
Ciencias Exactas Apoptosis Enterocytes Galectin-1 Mucosa Small bowel |
dc.description.none.fl_txt_mv |
Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function. Facultad de Ciencias Exactas |
description |
Abstract: Intestinal epithelial cells serve as mechanical barriers and active components of the mucosal immune system. These cells migrate from the crypt to the tip of the villus, where different stimuli can differentially affect their survival. Here we investigated, using in vitro and in vivo strategies, the role of galectin-1 (Gal-1), an evolutionarily conserved glycan-binding protein, in modulating the survival of human and mouse enterocytes. Both Gal-1 and its specific glyco-receptors were broadly expressed in small bowel enterocytes. Exogenous Gal-1 reduced the viability of enterocytes through apoptotic mechanisms involving activation of both caspase and mitochondrial pathways. Consistent with these findings, apoptotic cells were mainly detected at the tip of the villi, following administration of Gal-1. Moreover, Gal-1-deficient (Lgals-1-) mice showed longer villi compared with their wild-type counterparts in vivo. In an experimental model of starvation, fasted wild-type mice displayed reduced villi and lower intestinal weight compared with Lgals-1- mutant mice, an effect reflected by changes in the frequency of enterocyte apoptosis. Of note, human small bowel enterocytes were also prone to this pro-apoptotic effect. Thus, Gal-1 is broadly expressed in mucosal tissue and influences the viability of human and mouse enterocytes, an effect which might influence the migration of these cells from the crypt, the integrity of the villus and the epithelial barrier function. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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http://sedici.unlp.edu.ar/handle/10915/83984 |
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http://sedici.unlp.edu.ar/handle/10915/83984 |
dc.language.none.fl_str_mv |
eng |
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eng |
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info:eu-repo/semantics/altIdentifier/issn/2041-4889 info:eu-repo/semantics/altIdentifier/doi/10.1038/cddis.2011.44 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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