Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products
- Autores
- Bibiloni, Rodrigo; Gómez-Zavaglia, Andrea; De Antoni, Graciela Liliana
- Año de publicación
- 2001
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- An enzyme-based assay in combination with the most probable number (MPN) technique was developed for the enumeration of bifidobacteria. The assay employs the detection of fructose-6-phosphate phosphoketolase (F6PPK) activity as an indicator of the presence of bifidobacteria. The method was validated against viable counts and optimized with respect to selective media in order to quantitatively assess bifidobacteria in dairy products and other probiotic preparations. Several commercial products and homemade fermented milks were analyzed. Counts of bifidobacteria ranged from 10⁷ to 10⁸ MPN/ml in commercial products and homemade fermented milks Commercial starters provided by Argentinean industries had between 10⁷ and 10¹¹ MPN/ml. The results obtained in this study suggest that the combination of F6PPK activity determination and the MPN methodology allows an accurate determination of Bifidobacterium in pure cultures, dairy products, and other probiotic preparations.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Criotecnología de Alimentos - Materia
-
Ciencias Exactas
Biología
Bifidobacterium
Enzyme - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-nd/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/139269
Ver los metadatos del registro completo
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Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy ProductsBibiloni, RodrigoGómez-Zavaglia, AndreaDe Antoni, Graciela LilianaCiencias ExactasBiologíaBifidobacteriumEnzymeAn enzyme-based assay in combination with the most probable number (MPN) technique was developed for the enumeration of bifidobacteria. The assay employs the detection of fructose-6-phosphate phosphoketolase (F6PPK) activity as an indicator of the presence of bifidobacteria. The method was validated against viable counts and optimized with respect to selective media in order to quantitatively assess bifidobacteria in dairy products and other probiotic preparations. Several commercial products and homemade fermented milks were analyzed. Counts of bifidobacteria ranged from 10⁷ to 10⁸ MPN/ml in commercial products and homemade fermented milks Commercial starters provided by Argentinean industries had between 10⁷ and 10¹¹ MPN/ml. The results obtained in this study suggest that the combination of F6PPK activity determination and the MPN methodology allows an accurate determination of <i>Bifidobacterium</i> in pure cultures, dairy products, and other probiotic preparations.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Criotecnología de Alimentos2001-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf2001-2006http://sedici.unlp.edu.ar/handle/10915/139269enginfo:eu-repo/semantics/altIdentifier/issn/0362-028Xinfo:eu-repo/semantics/altIdentifier/issn/1944-9097info:eu-repo/semantics/altIdentifier/doi/10.4315/0362-028x-64.12.2001info:eu-repo/semantics/altIdentifier/pmid/11770630info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:09Zoai:sedici.unlp.edu.ar:10915/139269Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:09.559SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products |
| title |
Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products |
| spellingShingle |
Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products Bibiloni, Rodrigo Ciencias Exactas Biología Bifidobacterium Enzyme |
| title_short |
Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products |
| title_full |
Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products |
| title_fullStr |
Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products |
| title_full_unstemmed |
Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products |
| title_sort |
Enzyme-Based Most Probable Number Method for the Enumeration of <i>Bifidobacterium</i> in Dairy Products |
| dc.creator.none.fl_str_mv |
Bibiloni, Rodrigo Gómez-Zavaglia, Andrea De Antoni, Graciela Liliana |
| author |
Bibiloni, Rodrigo |
| author_facet |
Bibiloni, Rodrigo Gómez-Zavaglia, Andrea De Antoni, Graciela Liliana |
| author_role |
author |
| author2 |
Gómez-Zavaglia, Andrea De Antoni, Graciela Liliana |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Ciencias Exactas Biología Bifidobacterium Enzyme |
| topic |
Ciencias Exactas Biología Bifidobacterium Enzyme |
| dc.description.none.fl_txt_mv |
An enzyme-based assay in combination with the most probable number (MPN) technique was developed for the enumeration of bifidobacteria. The assay employs the detection of fructose-6-phosphate phosphoketolase (F6PPK) activity as an indicator of the presence of bifidobacteria. The method was validated against viable counts and optimized with respect to selective media in order to quantitatively assess bifidobacteria in dairy products and other probiotic preparations. Several commercial products and homemade fermented milks were analyzed. Counts of bifidobacteria ranged from 10⁷ to 10⁸ MPN/ml in commercial products and homemade fermented milks Commercial starters provided by Argentinean industries had between 10⁷ and 10¹¹ MPN/ml. The results obtained in this study suggest that the combination of F6PPK activity determination and the MPN methodology allows an accurate determination of <i>Bifidobacterium</i> in pure cultures, dairy products, and other probiotic preparations. Facultad de Ciencias Exactas Centro de Investigación y Desarrollo en Criotecnología de Alimentos |
| description |
An enzyme-based assay in combination with the most probable number (MPN) technique was developed for the enumeration of bifidobacteria. The assay employs the detection of fructose-6-phosphate phosphoketolase (F6PPK) activity as an indicator of the presence of bifidobacteria. The method was validated against viable counts and optimized with respect to selective media in order to quantitatively assess bifidobacteria in dairy products and other probiotic preparations. Several commercial products and homemade fermented milks were analyzed. Counts of bifidobacteria ranged from 10⁷ to 10⁸ MPN/ml in commercial products and homemade fermented milks Commercial starters provided by Argentinean industries had between 10⁷ and 10¹¹ MPN/ml. The results obtained in this study suggest that the combination of F6PPK activity determination and the MPN methodology allows an accurate determination of <i>Bifidobacterium</i> in pure cultures, dairy products, and other probiotic preparations. |
| publishDate |
2001 |
| dc.date.none.fl_str_mv |
2001-12 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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http://sedici.unlp.edu.ar/handle/10915/139269 |
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eng |
| language |
eng |
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