Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation

Autores
Gómez-Zavaglia, Andrea; Kociubinski, Guillermo Luis; Pérez, Pablo Fernando; De Antoni, Graciela Liliana
Año de publicación
1998
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Twenty-five Bifidobacterium strains isolated from infant feces were identified by sugar fermentation patterns and whole-cell protein analysis. Using gradient SDS-PAGE, six characteristic protein bands of the genus were detected in 40 strains of bifidobacteria but not in lactobacilli. Computerized numerical analysis enabled strains to be grouped in two main clusters. Strains of Bifidobacterium bifidum belong to a well-differentiated cluster that joins the cluster of the remaining species at 0.582 similarity. The predominant species among isolated strains from infant feces were B. bifidum, B. longum, and B. breve. Probiotic and technological indicators such as surface properties, inhibitory capacity, resistance to bile and low pH, and ability to grow under aerobic conditions were studied. Not all desirable characteristics were present in a single strain. In general, adherent and inhibitory strains were not resistant to bile, low pH, and aerobic conditions. Only 10 of 40 strains were resistant to 0.5% bile.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Química
Bifidobacterium
Probiotic
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/132489
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/132489
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network_name_str SEDICI (UNLP)
spelling Isolation and Characterization of Bifidobacterium Strains for Probiotic FormulationGómez-Zavaglia, AndreaKociubinski, Guillermo LuisPérez, Pablo FernandoDe Antoni, Graciela LilianaQuímicaBifidobacteriumProbioticTwenty-five Bifidobacterium strains isolated from infant feces were identified by sugar fermentation patterns and whole-cell protein analysis. Using gradient SDS-PAGE, six characteristic protein bands of the genus were detected in 40 strains of bifidobacteria but not in lactobacilli. Computerized numerical analysis enabled strains to be grouped in two main clusters. Strains of Bifidobacterium bifidum belong to a well-differentiated cluster that joins the cluster of the remaining species at 0.582 similarity. The predominant species among isolated strains from infant feces were B. bifidum, B. longum, and B. breve. Probiotic and technological indicators such as surface properties, inhibitory capacity, resistance to bile and low pH, and ability to grow under aerobic conditions were studied. Not all desirable characteristics were present in a single strain. In general, adherent and inhibitory strains were not resistant to bile, low pH, and aerobic conditions. Only 10 of 40 strains were resistant to 0.5% bile.Centro de Investigación y Desarrollo en Criotecnología de Alimentos1998info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf865-873http://sedici.unlp.edu.ar/handle/10915/132489enginfo:eu-repo/semantics/altIdentifier/issn/0362-028Xinfo:eu-repo/semantics/altIdentifier/issn/1944-9097info:eu-repo/semantics/altIdentifier/doi/10.4315/0362-028x-61.7.865info:eu-repo/semantics/altIdentifier/pmid/9678171info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:04:18Zoai:sedici.unlp.edu.ar:10915/132489Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:04:18.577SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
spellingShingle Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
Gómez-Zavaglia, Andrea
Química
Bifidobacterium
Probiotic
title_short Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title_full Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title_fullStr Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title_full_unstemmed Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
title_sort Isolation and Characterization of Bifidobacterium Strains for Probiotic Formulation
dc.creator.none.fl_str_mv Gómez-Zavaglia, Andrea
Kociubinski, Guillermo Luis
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
author Gómez-Zavaglia, Andrea
author_facet Gómez-Zavaglia, Andrea
Kociubinski, Guillermo Luis
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
author_role author
author2 Kociubinski, Guillermo Luis
Pérez, Pablo Fernando
De Antoni, Graciela Liliana
author2_role author
author
author
dc.subject.none.fl_str_mv Química
Bifidobacterium
Probiotic
topic Química
Bifidobacterium
Probiotic
dc.description.none.fl_txt_mv Twenty-five Bifidobacterium strains isolated from infant feces were identified by sugar fermentation patterns and whole-cell protein analysis. Using gradient SDS-PAGE, six characteristic protein bands of the genus were detected in 40 strains of bifidobacteria but not in lactobacilli. Computerized numerical analysis enabled strains to be grouped in two main clusters. Strains of Bifidobacterium bifidum belong to a well-differentiated cluster that joins the cluster of the remaining species at 0.582 similarity. The predominant species among isolated strains from infant feces were B. bifidum, B. longum, and B. breve. Probiotic and technological indicators such as surface properties, inhibitory capacity, resistance to bile and low pH, and ability to grow under aerobic conditions were studied. Not all desirable characteristics were present in a single strain. In general, adherent and inhibitory strains were not resistant to bile, low pH, and aerobic conditions. Only 10 of 40 strains were resistant to 0.5% bile.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description Twenty-five Bifidobacterium strains isolated from infant feces were identified by sugar fermentation patterns and whole-cell protein analysis. Using gradient SDS-PAGE, six characteristic protein bands of the genus were detected in 40 strains of bifidobacteria but not in lactobacilli. Computerized numerical analysis enabled strains to be grouped in two main clusters. Strains of Bifidobacterium bifidum belong to a well-differentiated cluster that joins the cluster of the remaining species at 0.582 similarity. The predominant species among isolated strains from infant feces were B. bifidum, B. longum, and B. breve. Probiotic and technological indicators such as surface properties, inhibitory capacity, resistance to bile and low pH, and ability to grow under aerobic conditions were studied. Not all desirable characteristics were present in a single strain. In general, adherent and inhibitory strains were not resistant to bile, low pH, and aerobic conditions. Only 10 of 40 strains were resistant to 0.5% bile.
publishDate 1998
dc.date.none.fl_str_mv 1998
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/132489
url http://sedici.unlp.edu.ar/handle/10915/132489
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0362-028X
info:eu-repo/semantics/altIdentifier/issn/1944-9097
info:eu-repo/semantics/altIdentifier/doi/10.4315/0362-028x-61.7.865
info:eu-repo/semantics/altIdentifier/pmid/9678171
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
865-873
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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