Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA
- Autores
- Vogler, Roberto Eugenio; Beltramino, Ariel Aníbal; Strong, Ellen E.; Rumi Macchi Zubiaurre, Alejandra; Peso, Juana G.
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Highly oxygenated freshwater habitats in the High Parana River (Argentina-Paraguay) were home to highly endemic snails of the genus Aylacostoma, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, A. chloroticum and A. brunneum, are currently included in an ongoing ex situ conservation programme, whereas A. guaraniticum and A. stigmaticum are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct A. stigmaticum by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the ex situ programme. We further use this gene to derive a secondary structure model, to examine the specific status of A. stigmaticum and to explore the evolutionary history of these snails. The secondary structure model based on A. stigmaticum revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct A. stigmaticum is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between A. chloroticum and A. brunneum, and estimated divergence times suggest that diversification of Aylacostoma in the High Parana River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.
Facultad de Ciencias Naturales y Museo - Materia
-
Ciencias Naturales
South American
Freshwater Snail - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/85901
Ver los metadatos del registro completo
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Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNAVogler, Roberto EugenioBeltramino, Ariel AníbalStrong, Ellen E.Rumi Macchi Zubiaurre, AlejandraPeso, Juana G.Ciencias NaturalesSouth AmericanFreshwater SnailHighly oxygenated freshwater habitats in the High Parana River (Argentina-Paraguay) were home to highly endemic snails of the genus <i>Aylacostoma</i>, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, <i>A. chloroticum</i> and <i>A. brunneum</i>, are currently included in an ongoing <i>ex situ</i> conservation programme, whereas <i>A. guaraniticum</i> and <i>A. stigmaticum</i> are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct <i>A. stigmaticum</i> by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the <i>ex situ</i> programme. We further use this gene to derive a secondary structure model, to examine the specific status of <i>A. stigmaticum</i> and to explore the evolutionary history of these snails. The secondary structure model based on <i>A. stigmaticum</i> revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct <i>A. stigmaticum</i> is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between <i>A. chloroticum</i> and <i>A. brunneum</i>, and estimated divergence times suggest that diversification of <i>Aylacostoma</i> in the High Parana River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.Facultad de Ciencias Naturales y Museo2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/85901enginfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0169191info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:08:36Zoai:sedici.unlp.edu.ar:10915/85901Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:08:36.971SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA |
title |
Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA |
spellingShingle |
Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA Vogler, Roberto Eugenio Ciencias Naturales South American Freshwater Snail |
title_short |
Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA |
title_full |
Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA |
title_fullStr |
Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA |
title_full_unstemmed |
Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA |
title_sort |
Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA |
dc.creator.none.fl_str_mv |
Vogler, Roberto Eugenio Beltramino, Ariel Aníbal Strong, Ellen E. Rumi Macchi Zubiaurre, Alejandra Peso, Juana G. |
author |
Vogler, Roberto Eugenio |
author_facet |
Vogler, Roberto Eugenio Beltramino, Ariel Aníbal Strong, Ellen E. Rumi Macchi Zubiaurre, Alejandra Peso, Juana G. |
author_role |
author |
author2 |
Beltramino, Ariel Aníbal Strong, Ellen E. Rumi Macchi Zubiaurre, Alejandra Peso, Juana G. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Ciencias Naturales South American Freshwater Snail |
topic |
Ciencias Naturales South American Freshwater Snail |
dc.description.none.fl_txt_mv |
Highly oxygenated freshwater habitats in the High Parana River (Argentina-Paraguay) were home to highly endemic snails of the genus <i>Aylacostoma</i>, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, <i>A. chloroticum</i> and <i>A. brunneum</i>, are currently included in an ongoing <i>ex situ</i> conservation programme, whereas <i>A. guaraniticum</i> and <i>A. stigmaticum</i> are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct <i>A. stigmaticum</i> by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the <i>ex situ</i> programme. We further use this gene to derive a secondary structure model, to examine the specific status of <i>A. stigmaticum</i> and to explore the evolutionary history of these snails. The secondary structure model based on <i>A. stigmaticum</i> revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct <i>A. stigmaticum</i> is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between <i>A. chloroticum</i> and <i>A. brunneum</i>, and estimated divergence times suggest that diversification of <i>Aylacostoma</i> in the High Parana River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods. Facultad de Ciencias Naturales y Museo |
description |
Highly oxygenated freshwater habitats in the High Parana River (Argentina-Paraguay) were home to highly endemic snails of the genus <i>Aylacostoma</i>, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, <i>A. chloroticum</i> and <i>A. brunneum</i>, are currently included in an ongoing <i>ex situ</i> conservation programme, whereas <i>A. guaraniticum</i> and <i>A. stigmaticum</i> are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct <i>A. stigmaticum</i> by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the <i>ex situ</i> programme. We further use this gene to derive a secondary structure model, to examine the specific status of <i>A. stigmaticum</i> and to explore the evolutionary history of these snails. The secondary structure model based on <i>A. stigmaticum</i> revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct <i>A. stigmaticum</i> is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between <i>A. chloroticum</i> and <i>A. brunneum</i>, and estimated divergence times suggest that diversification of <i>Aylacostoma</i> in the High Parana River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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eng |
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eng |
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