Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA

Autores
Vogler, Roberto Eugenio; Beltramino, Ariel Aníbal; Strong, Ellen E.; Rumi Macchi Zubiaurre, Alejandra; Peso, Juana G.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Highly oxygenated freshwater habitats in the High Parana River (Argentina-Paraguay) were home to highly endemic snails of the genus Aylacostoma, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, A. chloroticum and A. brunneum, are currently included in an ongoing ex situ conservation programme, whereas A. guaraniticum and A. stigmaticum are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct A. stigmaticum by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the ex situ programme. We further use this gene to derive a secondary structure model, to examine the specific status of A. stigmaticum and to explore the evolutionary history of these snails. The secondary structure model based on A. stigmaticum revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct A. stigmaticum is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between A. chloroticum and A. brunneum, and estimated divergence times suggest that diversification of Aylacostoma in the High Parana River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.
Facultad de Ciencias Naturales y Museo
Materia
Ciencias Naturales
South American
Freshwater Snail
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/85901

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network_name_str SEDICI (UNLP)
spelling Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNAVogler, Roberto EugenioBeltramino, Ariel AníbalStrong, Ellen E.Rumi Macchi Zubiaurre, AlejandraPeso, Juana G.Ciencias NaturalesSouth AmericanFreshwater SnailHighly oxygenated freshwater habitats in the High Parana River (Argentina-Paraguay) were home to highly endemic snails of the genus <i>Aylacostoma</i>, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, <i>A. chloroticum</i> and <i>A. brunneum</i>, are currently included in an ongoing <i>ex situ</i> conservation programme, whereas <i>A. guaraniticum</i> and <i>A. stigmaticum</i> are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct <i>A. stigmaticum</i> by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the <i>ex situ</i> programme. We further use this gene to derive a secondary structure model, to examine the specific status of <i>A. stigmaticum</i> and to explore the evolutionary history of these snails. The secondary structure model based on <i>A. stigmaticum</i> revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct <i>A. stigmaticum</i> is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between <i>A. chloroticum</i> and <i>A. brunneum</i>, and estimated divergence times suggest that diversification of <i>Aylacostoma</i> in the High Parana River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.Facultad de Ciencias Naturales y Museo2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/85901enginfo:eu-repo/semantics/altIdentifier/issn/1932-6203info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0169191info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:08:36Zoai:sedici.unlp.edu.ar:10915/85901Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:08:36.971SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA
title Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA
spellingShingle Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA
Vogler, Roberto Eugenio
Ciencias Naturales
South American
Freshwater Snail
title_short Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA
title_full Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA
title_fullStr Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA
title_full_unstemmed Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA
title_sort Insights into the evolutionary history of an extinct South American freshwater snail based on historical DNA
dc.creator.none.fl_str_mv Vogler, Roberto Eugenio
Beltramino, Ariel Aníbal
Strong, Ellen E.
Rumi Macchi Zubiaurre, Alejandra
Peso, Juana G.
author Vogler, Roberto Eugenio
author_facet Vogler, Roberto Eugenio
Beltramino, Ariel Aníbal
Strong, Ellen E.
Rumi Macchi Zubiaurre, Alejandra
Peso, Juana G.
author_role author
author2 Beltramino, Ariel Aníbal
Strong, Ellen E.
Rumi Macchi Zubiaurre, Alejandra
Peso, Juana G.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
South American
Freshwater Snail
topic Ciencias Naturales
South American
Freshwater Snail
dc.description.none.fl_txt_mv Highly oxygenated freshwater habitats in the High Parana River (Argentina-Paraguay) were home to highly endemic snails of the genus <i>Aylacostoma</i>, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, <i>A. chloroticum</i> and <i>A. brunneum</i>, are currently included in an ongoing <i>ex situ</i> conservation programme, whereas <i>A. guaraniticum</i> and <i>A. stigmaticum</i> are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct <i>A. stigmaticum</i> by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the <i>ex situ</i> programme. We further use this gene to derive a secondary structure model, to examine the specific status of <i>A. stigmaticum</i> and to explore the evolutionary history of these snails. The secondary structure model based on <i>A. stigmaticum</i> revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct <i>A. stigmaticum</i> is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between <i>A. chloroticum</i> and <i>A. brunneum</i>, and estimated divergence times suggest that diversification of <i>Aylacostoma</i> in the High Parana River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.
Facultad de Ciencias Naturales y Museo
description Highly oxygenated freshwater habitats in the High Parana River (Argentina-Paraguay) were home to highly endemic snails of the genus <i>Aylacostoma</i>, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, <i>A. chloroticum</i> and <i>A. brunneum</i>, are currently included in an ongoing <i>ex situ</i> conservation programme, whereas <i>A. guaraniticum</i> and <i>A. stigmaticum</i> are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct <i>A. stigmaticum</i> by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the <i>ex situ</i> programme. We further use this gene to derive a secondary structure model, to examine the specific status of <i>A. stigmaticum</i> and to explore the evolutionary history of these snails. The secondary structure model based on <i>A. stigmaticum</i> revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct <i>A. stigmaticum</i> is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between <i>A. chloroticum</i> and <i>A. brunneum</i>, and estimated divergence times suggest that diversification of <i>Aylacostoma</i> in the High Parana River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
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dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1932-6203
info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0169191
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Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
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Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
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