Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)

Autores
Horak, Cristina Natalia; Assef, Yanina Andrea
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
P-glycoprotein (P-gp) mediated multixenobioticresistance (MXR) is a mechanism analogous to multidrug resistance, which has been extensivelycharacterized in mammalian tumours. The expression and function of the MXR mechanism hasbeen demonstrated in numerous aquatic organisms and has been proposed as a biomarker forpollution assessment. A close relationship between thermal stress and MXR response has beenreported in some aquatic organisms. Seasonal studies in freshwater organisms are scarce andconducted mainly in zebra mussel (Dreissena polymorpha), whose presence has not been reportedin South America. The general purpose of the present study was to evaluate seasonal variation of abiomarker, the MXR mechanism, in the worldwide distributed freshwater snail P. acuta. Weanalyzed the in situ influence of temperature on the biomarker response over an 18-month fieldstudy. MXR defence system was evaluated by a combination of functional assays (RB accumulation)and molecular approaches to analyse P-gp expression. The results demonstrated a linear correlationbetween MXR response, at activity and expression level, and water temperature at sample site, in P.acuta snails. The characterization of the MXR system in worldwide distributed species, includingthe study of their seasonal fluctuations, could contribute to the increasing interest to incorporate thisbiomarker to provide an integrated assessment of mussel health status. This work supports thepossible use of P. acuta snails with this purpose and also highlights that the occurrence of variationsin MXR response related to water temperature has to be taken into account in the interpretation ofin situ monitoring studies
Fil: Horak, Cristina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; Argentina
Fil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; Argentina
Materia
MXR
P-GLYCOPROTEIN
BIOMARKER
TEMPERATURE
FRESHWATER SNAIL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/34998
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)Horak, Cristina NataliaAssef, Yanina AndreaMXRP-GLYCOPROTEINBIOMARKERTEMPERATUREFRESHWATER SNAILhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1P-glycoprotein (P-gp) mediated multixenobioticresistance (MXR) is a mechanism analogous to multidrug resistance, which has been extensivelycharacterized in mammalian tumours. The expression and function of the MXR mechanism hasbeen demonstrated in numerous aquatic organisms and has been proposed as a biomarker forpollution assessment. A close relationship between thermal stress and MXR response has beenreported in some aquatic organisms. Seasonal studies in freshwater organisms are scarce andconducted mainly in zebra mussel (Dreissena polymorpha), whose presence has not been reportedin South America. The general purpose of the present study was to evaluate seasonal variation of abiomarker, the MXR mechanism, in the worldwide distributed freshwater snail P. acuta. Weanalyzed the in situ influence of temperature on the biomarker response over an 18-month fieldstudy. MXR defence system was evaluated by a combination of functional assays (RB accumulation)and molecular approaches to analyse P-gp expression. The results demonstrated a linear correlationbetween MXR response, at activity and expression level, and water temperature at sample site, in P.acuta snails. The characterization of the MXR system in worldwide distributed species, includingthe study of their seasonal fluctuations, could contribute to the increasing interest to incorporate thisbiomarker to provide an integrated assessment of mussel health status. This work supports thepossible use of P. acuta snails with this purpose and also highlights that the occurrence of variationsin MXR response related to water temperature has to be taken into account in the interpretation ofin situ monitoring studiesFil: Horak, Cristina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; ArgentinaFil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; ArgentinaAcademia Sinica2017-10-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34998Horak, Cristina Natalia; Assef, Yanina Andrea; Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805); Academia Sinica; Zoological Studies; 56; 27; 2-10-2017; 1-111021-5506CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/Journals/56/56-27.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.6620/ZS.2017.56- 27info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:38:25Zoai:ri.conicet.gov.ar:11336/34998instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:38:25.377CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)
title Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)
spellingShingle Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)
Horak, Cristina Natalia
MXR
P-GLYCOPROTEIN
BIOMARKER
TEMPERATURE
FRESHWATER SNAIL
title_short Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)
title_full Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)
title_fullStr Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)
title_full_unstemmed Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)
title_sort Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)
dc.creator.none.fl_str_mv Horak, Cristina Natalia
Assef, Yanina Andrea
author Horak, Cristina Natalia
author_facet Horak, Cristina Natalia
Assef, Yanina Andrea
author_role author
author2 Assef, Yanina Andrea
author2_role author
dc.subject.none.fl_str_mv MXR
P-GLYCOPROTEIN
BIOMARKER
TEMPERATURE
FRESHWATER SNAIL
topic MXR
P-GLYCOPROTEIN
BIOMARKER
TEMPERATURE
FRESHWATER SNAIL
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv P-glycoprotein (P-gp) mediated multixenobioticresistance (MXR) is a mechanism analogous to multidrug resistance, which has been extensivelycharacterized in mammalian tumours. The expression and function of the MXR mechanism hasbeen demonstrated in numerous aquatic organisms and has been proposed as a biomarker forpollution assessment. A close relationship between thermal stress and MXR response has beenreported in some aquatic organisms. Seasonal studies in freshwater organisms are scarce andconducted mainly in zebra mussel (Dreissena polymorpha), whose presence has not been reportedin South America. The general purpose of the present study was to evaluate seasonal variation of abiomarker, the MXR mechanism, in the worldwide distributed freshwater snail P. acuta. Weanalyzed the in situ influence of temperature on the biomarker response over an 18-month fieldstudy. MXR defence system was evaluated by a combination of functional assays (RB accumulation)and molecular approaches to analyse P-gp expression. The results demonstrated a linear correlationbetween MXR response, at activity and expression level, and water temperature at sample site, in P.acuta snails. The characterization of the MXR system in worldwide distributed species, includingthe study of their seasonal fluctuations, could contribute to the increasing interest to incorporate thisbiomarker to provide an integrated assessment of mussel health status. This work supports thepossible use of P. acuta snails with this purpose and also highlights that the occurrence of variationsin MXR response related to water temperature has to be taken into account in the interpretation ofin situ monitoring studies
Fil: Horak, Cristina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; Argentina
Fil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; Argentina
description P-glycoprotein (P-gp) mediated multixenobioticresistance (MXR) is a mechanism analogous to multidrug resistance, which has been extensivelycharacterized in mammalian tumours. The expression and function of the MXR mechanism hasbeen demonstrated in numerous aquatic organisms and has been proposed as a biomarker forpollution assessment. A close relationship between thermal stress and MXR response has beenreported in some aquatic organisms. Seasonal studies in freshwater organisms are scarce andconducted mainly in zebra mussel (Dreissena polymorpha), whose presence has not been reportedin South America. The general purpose of the present study was to evaluate seasonal variation of abiomarker, the MXR mechanism, in the worldwide distributed freshwater snail P. acuta. Weanalyzed the in situ influence of temperature on the biomarker response over an 18-month fieldstudy. MXR defence system was evaluated by a combination of functional assays (RB accumulation)and molecular approaches to analyse P-gp expression. The results demonstrated a linear correlationbetween MXR response, at activity and expression level, and water temperature at sample site, in P.acuta snails. The characterization of the MXR system in worldwide distributed species, includingthe study of their seasonal fluctuations, could contribute to the increasing interest to incorporate thisbiomarker to provide an integrated assessment of mussel health status. This work supports thepossible use of P. acuta snails with this purpose and also highlights that the occurrence of variationsin MXR response related to water temperature has to be taken into account in the interpretation ofin situ monitoring studies
publishDate 2017
dc.date.none.fl_str_mv 2017-10-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/34998
Horak, Cristina Natalia; Assef, Yanina Andrea; Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805); Academia Sinica; Zoological Studies; 56; 27; 2-10-2017; 1-11
1021-5506
CONICET Digital
CONICET
url http://hdl.handle.net/11336/34998
identifier_str_mv Horak, Cristina Natalia; Assef, Yanina Andrea; Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805); Academia Sinica; Zoological Studies; 56; 27; 2-10-2017; 1-11
1021-5506
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/Journals/56/56-27.pdf
info:eu-repo/semantics/altIdentifier/doi/10.6620/ZS.2017.56- 27
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academia Sinica
publisher.none.fl_str_mv Academia Sinica
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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