Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)
- Autores
- Horak, Cristina Natalia; Assef, Yanina Andrea
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- P-glycoprotein (P-gp) mediated multixenobioticresistance (MXR) is a mechanism analogous to multidrug resistance, which has been extensivelycharacterized in mammalian tumours. The expression and function of the MXR mechanism hasbeen demonstrated in numerous aquatic organisms and has been proposed as a biomarker forpollution assessment. A close relationship between thermal stress and MXR response has beenreported in some aquatic organisms. Seasonal studies in freshwater organisms are scarce andconducted mainly in zebra mussel (Dreissena polymorpha), whose presence has not been reportedin South America. The general purpose of the present study was to evaluate seasonal variation of abiomarker, the MXR mechanism, in the worldwide distributed freshwater snail P. acuta. Weanalyzed the in situ influence of temperature on the biomarker response over an 18-month fieldstudy. MXR defence system was evaluated by a combination of functional assays (RB accumulation)and molecular approaches to analyse P-gp expression. The results demonstrated a linear correlationbetween MXR response, at activity and expression level, and water temperature at sample site, in P.acuta snails. The characterization of the MXR system in worldwide distributed species, includingthe study of their seasonal fluctuations, could contribute to the increasing interest to incorporate thisbiomarker to provide an integrated assessment of mussel health status. This work supports thepossible use of P. acuta snails with this purpose and also highlights that the occurrence of variationsin MXR response related to water temperature has to be taken into account in the interpretation ofin situ monitoring studies
Fil: Horak, Cristina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; Argentina
Fil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; Argentina - Materia
-
MXR
P-GLYCOPROTEIN
BIOMARKER
TEMPERATURE
FRESHWATER SNAIL - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/34998
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Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805)Horak, Cristina NataliaAssef, Yanina AndreaMXRP-GLYCOPROTEINBIOMARKERTEMPERATUREFRESHWATER SNAILhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1P-glycoprotein (P-gp) mediated multixenobioticresistance (MXR) is a mechanism analogous to multidrug resistance, which has been extensivelycharacterized in mammalian tumours. The expression and function of the MXR mechanism hasbeen demonstrated in numerous aquatic organisms and has been proposed as a biomarker forpollution assessment. A close relationship between thermal stress and MXR response has beenreported in some aquatic organisms. Seasonal studies in freshwater organisms are scarce andconducted mainly in zebra mussel (Dreissena polymorpha), whose presence has not been reportedin South America. The general purpose of the present study was to evaluate seasonal variation of abiomarker, the MXR mechanism, in the worldwide distributed freshwater snail P. acuta. Weanalyzed the in situ influence of temperature on the biomarker response over an 18-month fieldstudy. MXR defence system was evaluated by a combination of functional assays (RB accumulation)and molecular approaches to analyse P-gp expression. The results demonstrated a linear correlationbetween MXR response, at activity and expression level, and water temperature at sample site, in P.acuta snails. The characterization of the MXR system in worldwide distributed species, includingthe study of their seasonal fluctuations, could contribute to the increasing interest to incorporate thisbiomarker to provide an integrated assessment of mussel health status. This work supports thepossible use of P. acuta snails with this purpose and also highlights that the occurrence of variationsin MXR response related to water temperature has to be taken into account in the interpretation ofin situ monitoring studiesFil: Horak, Cristina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; ArgentinaFil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; ArgentinaAcademia Sinica2017-10-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/34998Horak, Cristina Natalia; Assef, Yanina Andrea; Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805); Academia Sinica; Zoological Studies; 56; 27; 2-10-2017; 1-111021-5506CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/Journals/56/56-27.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.6620/ZS.2017.56- 27info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:44:48Zoai:ri.conicet.gov.ar:11336/34998instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:44:49.112CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805) |
title |
Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805) |
spellingShingle |
Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805) Horak, Cristina Natalia MXR P-GLYCOPROTEIN BIOMARKER TEMPERATURE FRESHWATER SNAIL |
title_short |
Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805) |
title_full |
Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805) |
title_fullStr |
Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805) |
title_full_unstemmed |
Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805) |
title_sort |
Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805) |
dc.creator.none.fl_str_mv |
Horak, Cristina Natalia Assef, Yanina Andrea |
author |
Horak, Cristina Natalia |
author_facet |
Horak, Cristina Natalia Assef, Yanina Andrea |
author_role |
author |
author2 |
Assef, Yanina Andrea |
author2_role |
author |
dc.subject.none.fl_str_mv |
MXR P-GLYCOPROTEIN BIOMARKER TEMPERATURE FRESHWATER SNAIL |
topic |
MXR P-GLYCOPROTEIN BIOMARKER TEMPERATURE FRESHWATER SNAIL |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
P-glycoprotein (P-gp) mediated multixenobioticresistance (MXR) is a mechanism analogous to multidrug resistance, which has been extensivelycharacterized in mammalian tumours. The expression and function of the MXR mechanism hasbeen demonstrated in numerous aquatic organisms and has been proposed as a biomarker forpollution assessment. A close relationship between thermal stress and MXR response has beenreported in some aquatic organisms. Seasonal studies in freshwater organisms are scarce andconducted mainly in zebra mussel (Dreissena polymorpha), whose presence has not been reportedin South America. The general purpose of the present study was to evaluate seasonal variation of abiomarker, the MXR mechanism, in the worldwide distributed freshwater snail P. acuta. Weanalyzed the in situ influence of temperature on the biomarker response over an 18-month fieldstudy. MXR defence system was evaluated by a combination of functional assays (RB accumulation)and molecular approaches to analyse P-gp expression. The results demonstrated a linear correlationbetween MXR response, at activity and expression level, and water temperature at sample site, in P.acuta snails. The characterization of the MXR system in worldwide distributed species, includingthe study of their seasonal fluctuations, could contribute to the increasing interest to incorporate thisbiomarker to provide an integrated assessment of mussel health status. This work supports thepossible use of P. acuta snails with this purpose and also highlights that the occurrence of variationsin MXR response related to water temperature has to be taken into account in the interpretation ofin situ monitoring studies Fil: Horak, Cristina Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; Argentina Fil: Assef, Yanina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte. Centro de Investigación Esquel de Montaña y Estepa Patagóica. Universidad Nacional de la Patagonia ; Argentina |
description |
P-glycoprotein (P-gp) mediated multixenobioticresistance (MXR) is a mechanism analogous to multidrug resistance, which has been extensivelycharacterized in mammalian tumours. The expression and function of the MXR mechanism hasbeen demonstrated in numerous aquatic organisms and has been proposed as a biomarker forpollution assessment. A close relationship between thermal stress and MXR response has beenreported in some aquatic organisms. Seasonal studies in freshwater organisms are scarce andconducted mainly in zebra mussel (Dreissena polymorpha), whose presence has not been reportedin South America. The general purpose of the present study was to evaluate seasonal variation of abiomarker, the MXR mechanism, in the worldwide distributed freshwater snail P. acuta. Weanalyzed the in situ influence of temperature on the biomarker response over an 18-month fieldstudy. MXR defence system was evaluated by a combination of functional assays (RB accumulation)and molecular approaches to analyse P-gp expression. The results demonstrated a linear correlationbetween MXR response, at activity and expression level, and water temperature at sample site, in P.acuta snails. The characterization of the MXR system in worldwide distributed species, includingthe study of their seasonal fluctuations, could contribute to the increasing interest to incorporate thisbiomarker to provide an integrated assessment of mussel health status. This work supports thepossible use of P. acuta snails with this purpose and also highlights that the occurrence of variationsin MXR response related to water temperature has to be taken into account in the interpretation ofin situ monitoring studies |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-02 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/34998 Horak, Cristina Natalia; Assef, Yanina Andrea; Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805); Academia Sinica; Zoological Studies; 56; 27; 2-10-2017; 1-11 1021-5506 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/34998 |
identifier_str_mv |
Horak, Cristina Natalia; Assef, Yanina Andrea; Influence of Water Temperature on the MXR Activity and P-glycoprotein Expression in the Freshwater Snail, Physa acuta (Draparnaud, 1805); Academia Sinica; Zoological Studies; 56; 27; 2-10-2017; 1-11 1021-5506 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/http://zoolstud.sinica.edu.tw/Journals/56/56-27.pdf info:eu-repo/semantics/altIdentifier/doi/10.6620/ZS.2017.56- 27 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Academia Sinica |
publisher.none.fl_str_mv |
Academia Sinica |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844613409767686144 |
score |
13.070432 |