Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA

Autores
Vogler, Roberto Eugenio; Beltramino, Ariel Anibal; Strong, Ellen E.; Rumi Macchi Z., Alejandra; Peso, Juana Guadalupe
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Highly oxygenated freshwater habitats in the High Paraná River (Argentina–Paraguay) were home to highly endemic snails of the genus Aylacostoma, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, A. chloroticum and A. brunneum, are currently included in an ongoing ex situ conservation programme, whereas A. guaraniticum and A. stigmaticum are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct A. stigmaticum by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the ex situ programme. We further use this gene to derive a secondary structure model, to examine the specific status of A. stigmaticum and to explore the evolutionary history of these snails. The secondary structure model based on A. stigmaticum revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct A. stigmaticum is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between A. chloroticum and A. brunneum, and estimated divergence times suggest that diversification of Aylacostoma in the High Paraná River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.
Fil: Vogler, Roberto Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina
Fil: Beltramino, Ariel Anibal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Strong, Ellen E.. National Museum of Natural History; Estados Unidos
Fil: Rumi Macchi Z., Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peso, Juana Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
Materia
12S RRNA GENE
ABGD
AYLACOSTOMA
HIGH PARANA RIVER
K/THETA METHOD
SECONDARY STRUCTURE
THIARIDAE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/44377
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/44377
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNAVogler, Roberto EugenioBeltramino, Ariel AnibalStrong, Ellen E.Rumi Macchi Z., AlejandraPeso, Juana Guadalupe12S RRNA GENEABGDAYLACOSTOMAHIGH PARANA RIVERK/THETA METHODSECONDARY STRUCTURETHIARIDAEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Highly oxygenated freshwater habitats in the High Paraná River (Argentina–Paraguay) were home to highly endemic snails of the genus Aylacostoma, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, A. chloroticum and A. brunneum, are currently included in an ongoing ex situ conservation programme, whereas A. guaraniticum and A. stigmaticum are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct A. stigmaticum by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the ex situ programme. We further use this gene to derive a secondary structure model, to examine the specific status of A. stigmaticum and to explore the evolutionary history of these snails. The secondary structure model based on A. stigmaticum revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct A. stigmaticum is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between A. chloroticum and A. brunneum, and estimated divergence times suggest that diversification of Aylacostoma in the High Paraná River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.Fil: Vogler, Roberto Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; ArgentinaFil: Beltramino, Ariel Anibal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Strong, Ellen E.. National Museum of Natural History; Estados UnidosFil: Rumi Macchi Z., Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Peso, Juana Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; ArgentinaPublic Library of Science2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/44377Vogler, Roberto Eugenio; Beltramino, Ariel Anibal; Strong, Ellen E.; Rumi Macchi Z., Alejandra; Peso, Juana Guadalupe; Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA; Public Library of Science; Plos One; 11; 12; 12-2016; 1-16; e01691911932-6203CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0169191info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169191info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:45:50Zoai:ri.conicet.gov.ar:11336/44377instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:45:50.972CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA
title Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA
spellingShingle Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA
Vogler, Roberto Eugenio
12S RRNA GENE
ABGD
AYLACOSTOMA
HIGH PARANA RIVER
K/THETA METHOD
SECONDARY STRUCTURE
THIARIDAE
title_short Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA
title_full Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA
title_fullStr Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA
title_full_unstemmed Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA
title_sort Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA
dc.creator.none.fl_str_mv Vogler, Roberto Eugenio
Beltramino, Ariel Anibal
Strong, Ellen E.
Rumi Macchi Z., Alejandra
Peso, Juana Guadalupe
author Vogler, Roberto Eugenio
author_facet Vogler, Roberto Eugenio
Beltramino, Ariel Anibal
Strong, Ellen E.
Rumi Macchi Z., Alejandra
Peso, Juana Guadalupe
author_role author
author2 Beltramino, Ariel Anibal
Strong, Ellen E.
Rumi Macchi Z., Alejandra
Peso, Juana Guadalupe
author2_role author
author
author
author
dc.subject.none.fl_str_mv 12S RRNA GENE
ABGD
AYLACOSTOMA
HIGH PARANA RIVER
K/THETA METHOD
SECONDARY STRUCTURE
THIARIDAE
topic 12S RRNA GENE
ABGD
AYLACOSTOMA
HIGH PARANA RIVER
K/THETA METHOD
SECONDARY STRUCTURE
THIARIDAE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Highly oxygenated freshwater habitats in the High Paraná River (Argentina–Paraguay) were home to highly endemic snails of the genus Aylacostoma, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, A. chloroticum and A. brunneum, are currently included in an ongoing ex situ conservation programme, whereas A. guaraniticum and A. stigmaticum are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct A. stigmaticum by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the ex situ programme. We further use this gene to derive a secondary structure model, to examine the specific status of A. stigmaticum and to explore the evolutionary history of these snails. The secondary structure model based on A. stigmaticum revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct A. stigmaticum is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between A. chloroticum and A. brunneum, and estimated divergence times suggest that diversification of Aylacostoma in the High Paraná River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.
Fil: Vogler, Roberto Eugenio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina
Fil: Beltramino, Ariel Anibal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina. Universidad Nacional de Misiones. Facultad de Ciencias Exactas Químicas y Naturales. Departamento de Biología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Strong, Ellen E.. National Museum of Natural History; Estados Unidos
Fil: Rumi Macchi Z., Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. División de Zoología Invertebrados; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Peso, Juana Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina
description Highly oxygenated freshwater habitats in the High Paraná River (Argentina–Paraguay) were home to highly endemic snails of the genus Aylacostoma, which face extinction owing to the impoundment of the Yacyretá Reservoir in the 1990s. Two species, A. chloroticum and A. brunneum, are currently included in an ongoing ex situ conservation programme, whereas A. guaraniticum and A. stigmaticum are presumed extinct. Consequently, the validity and affinities of the latter two have remained enigmatic. Here, we provide the first molecular data on the extinct A. stigmaticum by means of historical DNA analysis. We describe patterns of molecular evolution based on partial sequences of the mitochondrial 12S ribosomal RNA gene from the extinct species and from those being bred within the ex situ programme. We further use this gene to derive a secondary structure model, to examine the specific status of A. stigmaticum and to explore the evolutionary history of these snails. The secondary structure model based on A. stigmaticum revealed that most polymorphic sites are located in unpaired regions. Our results support the view that the mitochondrial 12S region is an efficient marker for the discrimination of species, and the extinct A. stigmaticum is recognized here as a distinct evolutionary genetic species. Molecular phylogenetic analyses revealed a sister group relationship between A. chloroticum and A. brunneum, and estimated divergence times suggest that diversification of Aylacostoma in the High Paraná River might have started in the late Miocene via intra-basin speciation due to a past marine transgression. Finally, our findings reveal that DNA may be obtained from dried specimens at least 80 years after their collection, and confirms the feasibility of extracting historical DNA from museum collections for elucidating evolutionary patterns and processes in gastropods.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/44377
Vogler, Roberto Eugenio; Beltramino, Ariel Anibal; Strong, Ellen E.; Rumi Macchi Z., Alejandra; Peso, Juana Guadalupe; Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA; Public Library of Science; Plos One; 11; 12; 12-2016; 1-16; e0169191
1932-6203
CONICET Digital
CONICET
url http://hdl.handle.net/11336/44377
identifier_str_mv Vogler, Roberto Eugenio; Beltramino, Ariel Anibal; Strong, Ellen E.; Rumi Macchi Z., Alejandra; Peso, Juana Guadalupe; Insights into the Evolutionary History of an Extinct South American Freshwater Snail based on Historical DNA; Public Library of Science; Plos One; 11; 12; 12-2016; 1-16; e0169191
1932-6203
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
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info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0169191
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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