Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
- Autores
- Bernal, Alan Mauro; Fernandez-Brando, Romina Jimena; Bruballa, Andrea Cecilia; Fiorentino, Gabriela A.; Pineda, Gonzalo Ezequiel; Zotta, Elsa; Vermeulen, Mónica; Ramos, María Victoria; Rumbo, Martín; Palermo, Marina Sandra
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Enterohemorrhagic Escherichia coli (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of host-pathogen interactions, which involve resistance or tolerance mechanisms in the host that determine total host fitness and bacterial virulence. Taking advantage of the genetic differences between mouse strains, we analyzed the clinical progression in C57BL/6 and BALB/c weaned mice infected with an E. coli O157:H7 strain. We carefully analyzed colonization with several bacterial doses, clinical parameters, intestinal histology, and the integrity of the intestinal barrier, as well as local and systemic levels of antibodies to pathogenic factors. We demonstrated that although both strains had comparable susceptibility to Shiga toxin (Stx) and the intestinal bacterial burden was similar, C57BL/6 showed increased intestinal damage, alteration of the integrity of the intestinal barrier, and impaired renal function that resulted in increased mortality. The increased survival rate in the BALB/c strain was associated with an early specific antibody response as part of a tolerance mechanism.
Instituto de Estudios Inmunológicos y Fisiopatológicos - Materia
-
Medicina
Ciencias Exactas
EHEC
intestinal infection
mouse model
defense mechanisms
antibodies
Shiga toxins - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/141078
Ver los metadatos del registro completo
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Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance MechanismBernal, Alan MauroFernandez-Brando, Romina JimenaBruballa, Andrea CeciliaFiorentino, Gabriela A.Pineda, Gonzalo EzequielZotta, ElsaVermeulen, MónicaRamos, María VictoriaRumbo, MartínPalermo, Marina SandraMedicinaCiencias ExactasEHECintestinal infectionmouse modeldefense mechanismsantibodiesShiga toxinsEnterohemorrhagic <i>Escherichia coli</i> (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of host-pathogen interactions, which involve resistance or tolerance mechanisms in the host that determine total host fitness and bacterial virulence. Taking advantage of the genetic differences between mouse strains, we analyzed the clinical progression in C57BL/6 and BALB/c weaned mice infected with an <i>E. coli</i> O157:H7 strain. We carefully analyzed colonization with several bacterial doses, clinical parameters, intestinal histology, and the integrity of the intestinal barrier, as well as local and systemic levels of antibodies to pathogenic factors. We demonstrated that although both strains had comparable susceptibility to Shiga toxin (Stx) and the intestinal bacterial burden was similar, C57BL/6 showed increased intestinal damage, alteration of the integrity of the intestinal barrier, and impaired renal function that resulted in increased mortality. The increased survival rate in the BALB/c strain was associated with an early specific antibody response as part of a tolerance mechanism.Instituto de Estudios Inmunológicos y Fisiopatológicos2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/141078enginfo:eu-repo/semantics/altIdentifier/issn/1098-5522info:eu-repo/semantics/altIdentifier/issn/0019-9567info:eu-repo/semantics/altIdentifier/doi/10.1128/iai.00031-21info:eu-repo/semantics/altIdentifier/pmid/33619029info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:32Zoai:sedici.unlp.edu.ar:10915/141078Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:33.191SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
title |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
spellingShingle |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism Bernal, Alan Mauro Medicina Ciencias Exactas EHEC intestinal infection mouse model defense mechanisms antibodies Shiga toxins |
title_short |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
title_full |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
title_fullStr |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
title_full_unstemmed |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
title_sort |
Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism |
dc.creator.none.fl_str_mv |
Bernal, Alan Mauro Fernandez-Brando, Romina Jimena Bruballa, Andrea Cecilia Fiorentino, Gabriela A. Pineda, Gonzalo Ezequiel Zotta, Elsa Vermeulen, Mónica Ramos, María Victoria Rumbo, Martín Palermo, Marina Sandra |
author |
Bernal, Alan Mauro |
author_facet |
Bernal, Alan Mauro Fernandez-Brando, Romina Jimena Bruballa, Andrea Cecilia Fiorentino, Gabriela A. Pineda, Gonzalo Ezequiel Zotta, Elsa Vermeulen, Mónica Ramos, María Victoria Rumbo, Martín Palermo, Marina Sandra |
author_role |
author |
author2 |
Fernandez-Brando, Romina Jimena Bruballa, Andrea Cecilia Fiorentino, Gabriela A. Pineda, Gonzalo Ezequiel Zotta, Elsa Vermeulen, Mónica Ramos, María Victoria Rumbo, Martín Palermo, Marina Sandra |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Medicina Ciencias Exactas EHEC intestinal infection mouse model defense mechanisms antibodies Shiga toxins |
topic |
Medicina Ciencias Exactas EHEC intestinal infection mouse model defense mechanisms antibodies Shiga toxins |
dc.description.none.fl_txt_mv |
Enterohemorrhagic <i>Escherichia coli</i> (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of host-pathogen interactions, which involve resistance or tolerance mechanisms in the host that determine total host fitness and bacterial virulence. Taking advantage of the genetic differences between mouse strains, we analyzed the clinical progression in C57BL/6 and BALB/c weaned mice infected with an <i>E. coli</i> O157:H7 strain. We carefully analyzed colonization with several bacterial doses, clinical parameters, intestinal histology, and the integrity of the intestinal barrier, as well as local and systemic levels of antibodies to pathogenic factors. We demonstrated that although both strains had comparable susceptibility to Shiga toxin (Stx) and the intestinal bacterial burden was similar, C57BL/6 showed increased intestinal damage, alteration of the integrity of the intestinal barrier, and impaired renal function that resulted in increased mortality. The increased survival rate in the BALB/c strain was associated with an early specific antibody response as part of a tolerance mechanism. Instituto de Estudios Inmunológicos y Fisiopatológicos |
description |
Enterohemorrhagic <i>Escherichia coli</i> (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of host-pathogen interactions, which involve resistance or tolerance mechanisms in the host that determine total host fitness and bacterial virulence. Taking advantage of the genetic differences between mouse strains, we analyzed the clinical progression in C57BL/6 and BALB/c weaned mice infected with an <i>E. coli</i> O157:H7 strain. We carefully analyzed colonization with several bacterial doses, clinical parameters, intestinal histology, and the integrity of the intestinal barrier, as well as local and systemic levels of antibodies to pathogenic factors. We demonstrated that although both strains had comparable susceptibility to Shiga toxin (Stx) and the intestinal bacterial burden was similar, C57BL/6 showed increased intestinal damage, alteration of the integrity of the intestinal barrier, and impaired renal function that resulted in increased mortality. The increased survival rate in the BALB/c strain was associated with an early specific antibody response as part of a tolerance mechanism. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/141078 |
url |
http://sedici.unlp.edu.ar/handle/10915/141078 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
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openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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