Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism

Autores
Bernal, Alan Mauro; Fernandez-Brando, Romina Jimena; Bruballa, Andrea Cecilia; Fiorentino, Gabriela A.; Pineda, Gonzalo Ezequiel; Zotta, Elsa; Vermeulen, Mónica; Ramos, María Victoria; Rumbo, Martín; Palermo, Marina Sandra
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enterohemorrhagic Escherichia coli (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of host-pathogen interactions, which involve resistance or tolerance mechanisms in the host that determine total host fitness and bacterial virulence. Taking advantage of the genetic differences between mouse strains, we analyzed the clinical progression in C57BL/6 and BALB/c weaned mice infected with an E. coli O157:H7 strain. We carefully analyzed colonization with several bacterial doses, clinical parameters, intestinal histology, and the integrity of the intestinal barrier, as well as local and systemic levels of antibodies to pathogenic factors. We demonstrated that although both strains had comparable susceptibility to Shiga toxin (Stx) and the intestinal bacterial burden was similar, C57BL/6 showed increased intestinal damage, alteration of the integrity of the intestinal barrier, and impaired renal function that resulted in increased mortality. The increased survival rate in the BALB/c strain was associated with an early specific antibody response as part of a tolerance mechanism.
Instituto de Estudios Inmunológicos y Fisiopatológicos
Materia
Medicina
Ciencias Exactas
EHEC
intestinal infection
mouse model
defense mechanisms
antibodies
Shiga toxins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/141078

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network_name_str SEDICI (UNLP)
spelling Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance MechanismBernal, Alan MauroFernandez-Brando, Romina JimenaBruballa, Andrea CeciliaFiorentino, Gabriela A.Pineda, Gonzalo EzequielZotta, ElsaVermeulen, MónicaRamos, María VictoriaRumbo, MartínPalermo, Marina SandraMedicinaCiencias ExactasEHECintestinal infectionmouse modeldefense mechanismsantibodiesShiga toxinsEnterohemorrhagic <i>Escherichia coli</i> (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of host-pathogen interactions, which involve resistance or tolerance mechanisms in the host that determine total host fitness and bacterial virulence. Taking advantage of the genetic differences between mouse strains, we analyzed the clinical progression in C57BL/6 and BALB/c weaned mice infected with an <i>E. coli</i> O157:H7 strain. We carefully analyzed colonization with several bacterial doses, clinical parameters, intestinal histology, and the integrity of the intestinal barrier, as well as local and systemic levels of antibodies to pathogenic factors. We demonstrated that although both strains had comparable susceptibility to Shiga toxin (Stx) and the intestinal bacterial burden was similar, C57BL/6 showed increased intestinal damage, alteration of the integrity of the intestinal barrier, and impaired renal function that resulted in increased mortality. The increased survival rate in the BALB/c strain was associated with an early specific antibody response as part of a tolerance mechanism.Instituto de Estudios Inmunológicos y Fisiopatológicos2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/141078enginfo:eu-repo/semantics/altIdentifier/issn/1098-5522info:eu-repo/semantics/altIdentifier/issn/0019-9567info:eu-repo/semantics/altIdentifier/doi/10.1128/iai.00031-21info:eu-repo/semantics/altIdentifier/pmid/33619029info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:32:32Zoai:sedici.unlp.edu.ar:10915/141078Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:32:33.191SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
title Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
spellingShingle Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
Bernal, Alan Mauro
Medicina
Ciencias Exactas
EHEC
intestinal infection
mouse model
defense mechanisms
antibodies
Shiga toxins
title_short Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
title_full Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
title_fullStr Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
title_full_unstemmed Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
title_sort Differential Outcome between BALB/c and C57BL/6 Mice after <i>Escherichia coli</i> O157:H7 Infection Is Associated with a Dissimilar Tolerance Mechanism
dc.creator.none.fl_str_mv Bernal, Alan Mauro
Fernandez-Brando, Romina Jimena
Bruballa, Andrea Cecilia
Fiorentino, Gabriela A.
Pineda, Gonzalo Ezequiel
Zotta, Elsa
Vermeulen, Mónica
Ramos, María Victoria
Rumbo, Martín
Palermo, Marina Sandra
author Bernal, Alan Mauro
author_facet Bernal, Alan Mauro
Fernandez-Brando, Romina Jimena
Bruballa, Andrea Cecilia
Fiorentino, Gabriela A.
Pineda, Gonzalo Ezequiel
Zotta, Elsa
Vermeulen, Mónica
Ramos, María Victoria
Rumbo, Martín
Palermo, Marina Sandra
author_role author
author2 Fernandez-Brando, Romina Jimena
Bruballa, Andrea Cecilia
Fiorentino, Gabriela A.
Pineda, Gonzalo Ezequiel
Zotta, Elsa
Vermeulen, Mónica
Ramos, María Victoria
Rumbo, Martín
Palermo, Marina Sandra
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicina
Ciencias Exactas
EHEC
intestinal infection
mouse model
defense mechanisms
antibodies
Shiga toxins
topic Medicina
Ciencias Exactas
EHEC
intestinal infection
mouse model
defense mechanisms
antibodies
Shiga toxins
dc.description.none.fl_txt_mv Enterohemorrhagic <i>Escherichia coli</i> (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of host-pathogen interactions, which involve resistance or tolerance mechanisms in the host that determine total host fitness and bacterial virulence. Taking advantage of the genetic differences between mouse strains, we analyzed the clinical progression in C57BL/6 and BALB/c weaned mice infected with an <i>E. coli</i> O157:H7 strain. We carefully analyzed colonization with several bacterial doses, clinical parameters, intestinal histology, and the integrity of the intestinal barrier, as well as local and systemic levels of antibodies to pathogenic factors. We demonstrated that although both strains had comparable susceptibility to Shiga toxin (Stx) and the intestinal bacterial burden was similar, C57BL/6 showed increased intestinal damage, alteration of the integrity of the intestinal barrier, and impaired renal function that resulted in increased mortality. The increased survival rate in the BALB/c strain was associated with an early specific antibody response as part of a tolerance mechanism.
Instituto de Estudios Inmunológicos y Fisiopatológicos
description Enterohemorrhagic <i>Escherichia coli</i> (EHEC) infections can result in a wide range of clinical presentations despite that EHEC strains belong to the O157:H7 serotype, one of the most pathogenic forms. Although pathogen virulence influences disease outcome, we emphasize the concept of host-pathogen interactions, which involve resistance or tolerance mechanisms in the host that determine total host fitness and bacterial virulence. Taking advantage of the genetic differences between mouse strains, we analyzed the clinical progression in C57BL/6 and BALB/c weaned mice infected with an <i>E. coli</i> O157:H7 strain. We carefully analyzed colonization with several bacterial doses, clinical parameters, intestinal histology, and the integrity of the intestinal barrier, as well as local and systemic levels of antibodies to pathogenic factors. We demonstrated that although both strains had comparable susceptibility to Shiga toxin (Stx) and the intestinal bacterial burden was similar, C57BL/6 showed increased intestinal damage, alteration of the integrity of the intestinal barrier, and impaired renal function that resulted in increased mortality. The increased survival rate in the BALB/c strain was associated with an early specific antibody response as part of a tolerance mechanism.
publishDate 2021
dc.date.none.fl_str_mv 2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/141078
url http://sedici.unlp.edu.ar/handle/10915/141078
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1098-5522
info:eu-repo/semantics/altIdentifier/issn/0019-9567
info:eu-repo/semantics/altIdentifier/doi/10.1128/iai.00031-21
info:eu-repo/semantics/altIdentifier/pmid/33619029
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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