Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives

Autores
de Macedo Henrique, Izabella; Sacerdoti, Flavia; Lozzardo Ferreira, Raissa; Henrique, Camila; Amaral, María Marta; Fontes Piazza, Roxane María; Luz, Daniela
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Shiga toxins (Stx) are AB5-type toxins, composed of five B subunits which bind to Gb3 host cell receptors and an active A subunit, whose action on the ribosome leads to protein synthesis suppression. The two Stx types (Stx1 and Stx2) and their subtypes can be produced by Shiga toxin-producing Escherichia coli strains and some Shigella spp. These bacteria colonize the colon and induce diarrhea that may progress to hemorrhagic colitis and in the most severe cases, to hemolytic uremic syndrome, which could lead to death. Since the use of antibiotics in these infections is a topic of great controversy, the treatment remains supportive and there are no specific therapies to ameliorate the course. Therefore, there is an open window for Stx neutralization employing antibodies, which are versatile molecules. Indeed, polyclonal, monoclonal, and recombinant antibodies have been raised and tested in vitro and in vivo assays, showing differences in their neutralizing ability against deleterious effects of Stx. These molecules are in different phases of development for which we decide to present herein an updated report of these antibody molecules, their source, advantages, and disadvantages of the promising ones, as well as the challenges faced until reaching their applicability.
Fil: de Macedo Henrique, Izabella. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Lozzardo Ferreira, Raissa. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Henrique, Camila. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Fontes Piazza, Roxane María. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Luz, Daniela. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Materia
ANTIBODIES
SHIGA TOXIN-PRODUCING E. COLI
STX TOXINS
THERAPY
TRENDS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/204054

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network_name_str CONICET Digital (CONICET)
spelling Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectivesde Macedo Henrique, IzabellaSacerdoti, FlaviaLozzardo Ferreira, RaissaHenrique, CamilaAmaral, María MartaFontes Piazza, Roxane MaríaLuz, DanielaANTIBODIESSHIGA TOXIN-PRODUCING E. COLISTX TOXINSTHERAPYTRENDShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Shiga toxins (Stx) are AB5-type toxins, composed of five B subunits which bind to Gb3 host cell receptors and an active A subunit, whose action on the ribosome leads to protein synthesis suppression. The two Stx types (Stx1 and Stx2) and their subtypes can be produced by Shiga toxin-producing Escherichia coli strains and some Shigella spp. These bacteria colonize the colon and induce diarrhea that may progress to hemorrhagic colitis and in the most severe cases, to hemolytic uremic syndrome, which could lead to death. Since the use of antibiotics in these infections is a topic of great controversy, the treatment remains supportive and there are no specific therapies to ameliorate the course. Therefore, there is an open window for Stx neutralization employing antibodies, which are versatile molecules. Indeed, polyclonal, monoclonal, and recombinant antibodies have been raised and tested in vitro and in vivo assays, showing differences in their neutralizing ability against deleterious effects of Stx. These molecules are in different phases of development for which we decide to present herein an updated report of these antibody molecules, their source, advantages, and disadvantages of the promising ones, as well as the challenges faced until reaching their applicability.Fil: de Macedo Henrique, Izabella. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Lozzardo Ferreira, Raissa. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Henrique, Camila. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; ArgentinaFil: Fontes Piazza, Roxane María. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Luz, Daniela. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFrontiers Media2022-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/204054de Macedo Henrique, Izabella; Sacerdoti, Flavia; Lozzardo Ferreira, Raissa; Henrique, Camila; Amaral, María Marta; et al.; Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives; Frontiers Media; Frontiers in Cellular and Infection Microbiology; 12; 2-2022; 1-152235-2988CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fcimb.2022.825856/fullinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2022.825856info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:31:10Zoai:ri.conicet.gov.ar:11336/204054instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:31:11.176CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives
title Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives
spellingShingle Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives
de Macedo Henrique, Izabella
ANTIBODIES
SHIGA TOXIN-PRODUCING E. COLI
STX TOXINS
THERAPY
TRENDS
title_short Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives
title_full Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives
title_fullStr Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives
title_full_unstemmed Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives
title_sort Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives
dc.creator.none.fl_str_mv de Macedo Henrique, Izabella
Sacerdoti, Flavia
Lozzardo Ferreira, Raissa
Henrique, Camila
Amaral, María Marta
Fontes Piazza, Roxane María
Luz, Daniela
author de Macedo Henrique, Izabella
author_facet de Macedo Henrique, Izabella
Sacerdoti, Flavia
Lozzardo Ferreira, Raissa
Henrique, Camila
Amaral, María Marta
Fontes Piazza, Roxane María
Luz, Daniela
author_role author
author2 Sacerdoti, Flavia
Lozzardo Ferreira, Raissa
Henrique, Camila
Amaral, María Marta
Fontes Piazza, Roxane María
Luz, Daniela
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ANTIBODIES
SHIGA TOXIN-PRODUCING E. COLI
STX TOXINS
THERAPY
TRENDS
topic ANTIBODIES
SHIGA TOXIN-PRODUCING E. COLI
STX TOXINS
THERAPY
TRENDS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Shiga toxins (Stx) are AB5-type toxins, composed of five B subunits which bind to Gb3 host cell receptors and an active A subunit, whose action on the ribosome leads to protein synthesis suppression. The two Stx types (Stx1 and Stx2) and their subtypes can be produced by Shiga toxin-producing Escherichia coli strains and some Shigella spp. These bacteria colonize the colon and induce diarrhea that may progress to hemorrhagic colitis and in the most severe cases, to hemolytic uremic syndrome, which could lead to death. Since the use of antibiotics in these infections is a topic of great controversy, the treatment remains supportive and there are no specific therapies to ameliorate the course. Therefore, there is an open window for Stx neutralization employing antibodies, which are versatile molecules. Indeed, polyclonal, monoclonal, and recombinant antibodies have been raised and tested in vitro and in vivo assays, showing differences in their neutralizing ability against deleterious effects of Stx. These molecules are in different phases of development for which we decide to present herein an updated report of these antibody molecules, their source, advantages, and disadvantages of the promising ones, as well as the challenges faced until reaching their applicability.
Fil: de Macedo Henrique, Izabella. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Sacerdoti, Flavia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Lozzardo Ferreira, Raissa. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Henrique, Camila. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Amaral, María Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Fisiología y Biofísica Bernardo Houssay. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Fisiología y Biofísica Bernardo Houssay; Argentina
Fil: Fontes Piazza, Roxane María. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Luz, Daniela. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
description Shiga toxins (Stx) are AB5-type toxins, composed of five B subunits which bind to Gb3 host cell receptors and an active A subunit, whose action on the ribosome leads to protein synthesis suppression. The two Stx types (Stx1 and Stx2) and their subtypes can be produced by Shiga toxin-producing Escherichia coli strains and some Shigella spp. These bacteria colonize the colon and induce diarrhea that may progress to hemorrhagic colitis and in the most severe cases, to hemolytic uremic syndrome, which could lead to death. Since the use of antibiotics in these infections is a topic of great controversy, the treatment remains supportive and there are no specific therapies to ameliorate the course. Therefore, there is an open window for Stx neutralization employing antibodies, which are versatile molecules. Indeed, polyclonal, monoclonal, and recombinant antibodies have been raised and tested in vitro and in vivo assays, showing differences in their neutralizing ability against deleterious effects of Stx. These molecules are in different phases of development for which we decide to present herein an updated report of these antibody molecules, their source, advantages, and disadvantages of the promising ones, as well as the challenges faced until reaching their applicability.
publishDate 2022
dc.date.none.fl_str_mv 2022-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/204054
de Macedo Henrique, Izabella; Sacerdoti, Flavia; Lozzardo Ferreira, Raissa; Henrique, Camila; Amaral, María Marta; et al.; Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives; Frontiers Media; Frontiers in Cellular and Infection Microbiology; 12; 2-2022; 1-15
2235-2988
CONICET Digital
CONICET
url http://hdl.handle.net/11336/204054
identifier_str_mv de Macedo Henrique, Izabella; Sacerdoti, Flavia; Lozzardo Ferreira, Raissa; Henrique, Camila; Amaral, María Marta; et al.; Therapeutic Antibodies Against Shiga Toxins: Trends and Perspectives; Frontiers Media; Frontiers in Cellular and Infection Microbiology; 12; 2-2022; 1-15
2235-2988
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fcimb.2022.825856/full
info:eu-repo/semantics/altIdentifier/doi/10.3389/fcimb.2022.825856
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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