A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice

Autores
Byrne, Alana Brooke; García, Ayelén G.; Brahamian, Jorge Martin; Mauri, Aldana; Ferretti, Adrián; Polack, Fernando Pedro; Talarico, Laura Beatriz
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Dengue is a significant public health concern across tropical and subtropical regions worldwide, principally causing disease in children. Very young children are at increased risk of severe manifestations of dengue infection. The mechanism of dengue disease in this population is not fully understood. In this study, we present a murine model of dengue virus primary infection in suckling C57BL/6 and BALB/c mice in order to investigate disease pathogenesis. Three‐day‐old C57BL/6 mice intraperitoneally infected with DENV‐2 NGC were more susceptible to infection than BALB/c mice, showing increased liver enzymes, extended viremia, dissemination to organs and histological alterations in liver and small intestine. Furthermore, the immune response in DENV‐infected C57BL/6 mice exhibited a marked Th1 bias compared to BALB/c mice. These findings highlight the possibility of establishing an immunocompetent mouse model of DENV‐2 infection in suckling mice that reproduces certain signs of disease observed in humans and that could be used to further study age‐related mechanisms of dengue pathogenesis.
Fil: Byrne, Alana Brooke. Fundación para la Investigación en Infectología Infantil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García, Ayelén G.. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Brahamian, Jorge Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Mauri, Aldana. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Ferretti, Adrián. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Polack, Fernando Pedro. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Talarico, Laura Beatriz. Fundación para la Investigación en Infectología Infantil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
DENGUE
MOUSE MODEL
SUCKLING MICE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/144239
Acceder
id CONICETDig_499fbcc84eb3fe735611a95597c59ba5
oai_identifier_str oai:ri.conicet.gov.ar:11336/144239
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A murine model of dengue virus infection in suckling C57BL/6 and BALB/c miceByrne, Alana BrookeGarcía, Ayelén G.Brahamian, Jorge MartinMauri, AldanaFerretti, AdriánPolack, Fernando PedroTalarico, Laura BeatrizDENGUEMOUSE MODELSUCKLING MICEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Dengue is a significant public health concern across tropical and subtropical regions worldwide, principally causing disease in children. Very young children are at increased risk of severe manifestations of dengue infection. The mechanism of dengue disease in this population is not fully understood. In this study, we present a murine model of dengue virus primary infection in suckling C57BL/6 and BALB/c mice in order to investigate disease pathogenesis. Three‐day‐old C57BL/6 mice intraperitoneally infected with DENV‐2 NGC were more susceptible to infection than BALB/c mice, showing increased liver enzymes, extended viremia, dissemination to organs and histological alterations in liver and small intestine. Furthermore, the immune response in DENV‐infected C57BL/6 mice exhibited a marked Th1 bias compared to BALB/c mice. These findings highlight the possibility of establishing an immunocompetent mouse model of DENV‐2 infection in suckling mice that reproduces certain signs of disease observed in humans and that could be used to further study age‐related mechanisms of dengue pathogenesis.Fil: Byrne, Alana Brooke. Fundación para la Investigación en Infectología Infantil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: García, Ayelén G.. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Brahamian, Jorge Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Mauri, Aldana. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Ferretti, Adrián. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Polack, Fernando Pedro. Fundación para la Investigación en Infectología Infantil; ArgentinaFil: Talarico, Laura Beatriz. Fundación para la Investigación en Infectología Infantil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaJohn Wiley & Sons Inc2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/144239Byrne, Alana Brooke; García, Ayelén G.; Brahamian, Jorge Martin; Mauri, Aldana; Ferretti, Adrián; et al.; A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice; John Wiley & Sons Inc; Animal Models and Experimental Medicine; 12-2020; 1-112576-2095CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/ame2.12145info:eu-repo/semantics/altIdentifier/doi/10.1002/ame2.12145info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:43Zoai:ri.conicet.gov.ar:11336/144239instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:44.132CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice
title A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice
spellingShingle A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice
Byrne, Alana Brooke
DENGUE
MOUSE MODEL
SUCKLING MICE
title_short A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice
title_full A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice
title_fullStr A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice
title_full_unstemmed A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice
title_sort A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice
dc.creator.none.fl_str_mv Byrne, Alana Brooke
García, Ayelén G.
Brahamian, Jorge Martin
Mauri, Aldana
Ferretti, Adrián
Polack, Fernando Pedro
Talarico, Laura Beatriz
author Byrne, Alana Brooke
author_facet Byrne, Alana Brooke
García, Ayelén G.
Brahamian, Jorge Martin
Mauri, Aldana
Ferretti, Adrián
Polack, Fernando Pedro
Talarico, Laura Beatriz
author_role author
author2 García, Ayelén G.
Brahamian, Jorge Martin
Mauri, Aldana
Ferretti, Adrián
Polack, Fernando Pedro
Talarico, Laura Beatriz
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv DENGUE
MOUSE MODEL
SUCKLING MICE
topic DENGUE
MOUSE MODEL
SUCKLING MICE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Dengue is a significant public health concern across tropical and subtropical regions worldwide, principally causing disease in children. Very young children are at increased risk of severe manifestations of dengue infection. The mechanism of dengue disease in this population is not fully understood. In this study, we present a murine model of dengue virus primary infection in suckling C57BL/6 and BALB/c mice in order to investigate disease pathogenesis. Three‐day‐old C57BL/6 mice intraperitoneally infected with DENV‐2 NGC were more susceptible to infection than BALB/c mice, showing increased liver enzymes, extended viremia, dissemination to organs and histological alterations in liver and small intestine. Furthermore, the immune response in DENV‐infected C57BL/6 mice exhibited a marked Th1 bias compared to BALB/c mice. These findings highlight the possibility of establishing an immunocompetent mouse model of DENV‐2 infection in suckling mice that reproduces certain signs of disease observed in humans and that could be used to further study age‐related mechanisms of dengue pathogenesis.
Fil: Byrne, Alana Brooke. Fundación para la Investigación en Infectología Infantil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García, Ayelén G.. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Brahamian, Jorge Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Mauri, Aldana. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Ferretti, Adrián. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Polack, Fernando Pedro. Fundación para la Investigación en Infectología Infantil; Argentina
Fil: Talarico, Laura Beatriz. Fundación para la Investigación en Infectología Infantil; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Dengue is a significant public health concern across tropical and subtropical regions worldwide, principally causing disease in children. Very young children are at increased risk of severe manifestations of dengue infection. The mechanism of dengue disease in this population is not fully understood. In this study, we present a murine model of dengue virus primary infection in suckling C57BL/6 and BALB/c mice in order to investigate disease pathogenesis. Three‐day‐old C57BL/6 mice intraperitoneally infected with DENV‐2 NGC were more susceptible to infection than BALB/c mice, showing increased liver enzymes, extended viremia, dissemination to organs and histological alterations in liver and small intestine. Furthermore, the immune response in DENV‐infected C57BL/6 mice exhibited a marked Th1 bias compared to BALB/c mice. These findings highlight the possibility of establishing an immunocompetent mouse model of DENV‐2 infection in suckling mice that reproduces certain signs of disease observed in humans and that could be used to further study age‐related mechanisms of dengue pathogenesis.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/144239
Byrne, Alana Brooke; García, Ayelén G.; Brahamian, Jorge Martin; Mauri, Aldana; Ferretti, Adrián; et al.; A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice; John Wiley & Sons Inc; Animal Models and Experimental Medicine; 12-2020; 1-11
2576-2095
CONICET Digital
CONICET
url http://hdl.handle.net/11336/144239
identifier_str_mv Byrne, Alana Brooke; García, Ayelén G.; Brahamian, Jorge Martin; Mauri, Aldana; Ferretti, Adrián; et al.; A murine model of dengue virus infection in suckling C57BL/6 and BALB/c mice; John Wiley & Sons Inc; Animal Models and Experimental Medicine; 12-2020; 1-11
2576-2095
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1002/ame2.12145
info:eu-repo/semantics/altIdentifier/doi/10.1002/ame2.12145
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Inc
publisher.none.fl_str_mv John Wiley & Sons Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613458354503680
score 13.070432

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