Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects

Autores
Rozas Dennis, Gabriela Susana; Martí, Gerardo Aníbal; González, Nadia Lorena; Guhl, Felipe; Guérin, Diego Marcelo Alejandro
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.
Centro de Estudios Parasitológicos y de Vectores
Materia
Ciencias Naturales
Chagas disease
Inoculation protocol
Intrahaemocoelic inoculation
Triatoma virus
Triatomines
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/93400
Acceder
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network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insectsRozas Dennis, Gabriela SusanaMartí, Gerardo AníbalGonzález, Nadia LorenaGuhl, FelipeGuérin, Diego Marcelo AlejandroCiencias NaturalesChagas diseaseInoculation protocolIntrahaemocoelic inoculationTriatoma virusTriatominesVectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.Centro de Estudios Parasitológicos y de Vectores2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1-6http://sedici.unlp.edu.ar/handle/10915/93400enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/handle/11336/49114info:eu-repo/semantics/altIdentifier/url/http://www.jbmethods.org/jbm/article/view/46info:eu-repo/semantics/altIdentifier/issn/2326-9901info:eu-repo/semantics/altIdentifier/doi/10.14440/jbm.2016.46info:eu-repo/semantics/altIdentifier/hdl/11336/49114info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:19:17Zoai:sedici.unlp.edu.ar:10915/93400Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:19:17.402SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
spellingShingle Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
Rozas Dennis, Gabriela Susana
Ciencias Naturales
Chagas disease
Inoculation protocol
Intrahaemocoelic inoculation
Triatoma virus
Triatomines
title_short Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title_full Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title_fullStr Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title_full_unstemmed Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title_sort Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
dc.creator.none.fl_str_mv Rozas Dennis, Gabriela Susana
Martí, Gerardo Aníbal
González, Nadia Lorena
Guhl, Felipe
Guérin, Diego Marcelo Alejandro
author Rozas Dennis, Gabriela Susana
author_facet Rozas Dennis, Gabriela Susana
Martí, Gerardo Aníbal
González, Nadia Lorena
Guhl, Felipe
Guérin, Diego Marcelo Alejandro
author_role author
author2 Martí, Gerardo Aníbal
González, Nadia Lorena
Guhl, Felipe
Guérin, Diego Marcelo Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Naturales
Chagas disease
Inoculation protocol
Intrahaemocoelic inoculation
Triatoma virus
Triatomines
topic Ciencias Naturales
Chagas disease
Inoculation protocol
Intrahaemocoelic inoculation
Triatoma virus
Triatomines
dc.description.none.fl_txt_mv Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.
Centro de Estudios Parasitológicos y de Vectores
description Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/93400
url http://sedici.unlp.edu.ar/handle/10915/93400
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/handle/11336/49114
info:eu-repo/semantics/altIdentifier/url/http://www.jbmethods.org/jbm/article/view/46
info:eu-repo/semantics/altIdentifier/issn/2326-9901
info:eu-repo/semantics/altIdentifier/doi/10.14440/jbm.2016.46
info:eu-repo/semantics/altIdentifier/hdl/11336/49114
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1-6
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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