Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects

Autores
Rozas Dennis, Gabriela Susana; Marti, Gerardo Anibal; González, Nadia Lorena; Guhl, Felipe; Guérin, Diego Marcelo Alejandro
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.
Fil: Rozas Dennis, Gabriela Susana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: González, Nadia Lorena. Universidad de los Andes; Colombia
Fil: Guhl, Felipe. Universidad de los Andes; Colombia
Fil: Guérin, Diego Marcelo Alejandro. Universidad del País Vasco; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Chagas disease
inoculation protocol
intrahaemocoelic inoculation
Triatoma virus
Triatomines
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/49114
Acceder
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network_acronym_str CONICETDig
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network_name_str CONICET Digital (CONICET)
spelling Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insectsRozas Dennis, Gabriela SusanaMarti, Gerardo AnibalGonzález, Nadia LorenaGuhl, FelipeGuérin, Diego Marcelo AlejandroChagas diseaseinoculation protocolintrahaemocoelic inoculationTriatoma virusTriatomineshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.Fil: Rozas Dennis, Gabriela Susana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; ArgentinaFil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; ArgentinaFil: González, Nadia Lorena. Universidad de los Andes; ColombiaFil: Guhl, Felipe. Universidad de los Andes; ColombiaFil: Guérin, Diego Marcelo Alejandro. Universidad del País Vasco; España. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPOL Scientific2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/49114Rozas Dennis, Gabriela Susana; Marti, Gerardo Anibal; González, Nadia Lorena; Guhl, Felipe; Guérin, Diego Marcelo Alejandro; Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects; POL Scientific; Journal of Biological Methods; 3; 2; 5-2016; 1-6; e402326-9901CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.jbmethods.org/jbm/article/view/46info:eu-repo/semantics/altIdentifier/doi/10.14440/jbm.2016.46info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:14:49Zoai:ri.conicet.gov.ar:11336/49114instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:14:49.737CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
spellingShingle Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
Rozas Dennis, Gabriela Susana
Chagas disease
inoculation protocol
intrahaemocoelic inoculation
Triatoma virus
Triatomines
title_short Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title_full Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title_fullStr Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title_full_unstemmed Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
title_sort Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects
dc.creator.none.fl_str_mv Rozas Dennis, Gabriela Susana
Marti, Gerardo Anibal
González, Nadia Lorena
Guhl, Felipe
Guérin, Diego Marcelo Alejandro
author Rozas Dennis, Gabriela Susana
author_facet Rozas Dennis, Gabriela Susana
Marti, Gerardo Anibal
González, Nadia Lorena
Guhl, Felipe
Guérin, Diego Marcelo Alejandro
author_role author
author2 Marti, Gerardo Anibal
González, Nadia Lorena
Guhl, Felipe
Guérin, Diego Marcelo Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Chagas disease
inoculation protocol
intrahaemocoelic inoculation
Triatoma virus
Triatomines
topic Chagas disease
inoculation protocol
intrahaemocoelic inoculation
Triatoma virus
Triatomines
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.
Fil: Rozas Dennis, Gabriela Susana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina
Fil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Estudios Parasitológicos y de Vectores. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Centro de Estudios Parasitológicos y de Vectores; Argentina
Fil: González, Nadia Lorena. Universidad de los Andes; Colombia
Fil: Guhl, Felipe. Universidad de los Andes; Colombia
Fil: Guérin, Diego Marcelo Alejandro. Universidad del País Vasco; España. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Vectors of Chagas disease are currently controlled by employing several chemical insecticides though there is a continuing search for alternative ecological methods against disease causing vectors. An effective method includes the use of specific pathogens as biological control agents. The aim of this work was to describe a complete experimental inoculation protocol in triatomines. The intrahaemocoelic inoculation technique can be applied to inoculate different kinds of microorganisms such as viruses, fungi, bacteria and protozoa; so it could be considered a useful tool in infective bioassays. This article includes results from evaluations of Triatoma virus (TrV, Dicistroviridae: Cripavirus) infectivity in several triatomine species. The protocol, also suitable for any other kind of insects, describes the materials and steps required to safely inoculate the insects, preventing any damage and/or contamination.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/49114
Rozas Dennis, Gabriela Susana; Marti, Gerardo Anibal; González, Nadia Lorena; Guhl, Felipe; Guérin, Diego Marcelo Alejandro; Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects; POL Scientific; Journal of Biological Methods; 3; 2; 5-2016; 1-6; e40
2326-9901
CONICET Digital
CONICET
url http://hdl.handle.net/11336/49114
identifier_str_mv Rozas Dennis, Gabriela Susana; Marti, Gerardo Anibal; González, Nadia Lorena; Guhl, Felipe; Guérin, Diego Marcelo Alejandro; Triatomines intrahaemocoelic inoculation protocol: a useful tool to check infectivity in insects; POL Scientific; Journal of Biological Methods; 3; 2; 5-2016; 1-6; e40
2326-9901
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.jbmethods.org/jbm/article/view/46
info:eu-repo/semantics/altIdentifier/doi/10.14440/jbm.2016.46
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv POL Scientific
publisher.none.fl_str_mv POL Scientific
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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