Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils

Autores
Becerra, Alejandra Gabriela; Menoyo, Eugenia; Cabello, Marta Noemí; Pardo, Alejandro Guillermo; Salazar, María Julieta
Año de publicación
2022
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
The city of Córdoba is one of the most polluted cities in Argentina, with a large number of industrial plants located in urban and suburban areas. At 18 km from the capital of Córdoba is the town of Bouwer, considered one of the areas most affected by heavy metal contamination. An acid battery recycling factory dedicated to the recovery of lead (Pb) operated irregularly between 1984 and 2005. The smelter emitted Pb into the air and leaved a large amount of slag, used by neighbors to fill and level land. This caused numerous cases of Pb poisoning. Given the health risks and residence times of pollutants, it is necessary to apply remediation measures such as phytoremediation. For example the indigenous plant, B. pilosa, is a metalophytic plant species adapted to the climatic conditions and the soil of the area. The same behavior has been observed with native Arbuscular Mycorrhizal Fungi (AMF), which are important to be used as inoculants in remediation programs. Therefore, is important to select AMF species adapted to these contaminated sites. In the present work, the inoculation of B. pilosa seedlings with native AMF spores from Bouwer was evaluated. Seeds of B. pilosa collected in the field were sterilized and rinsed with sterile water. Seedlings were grown in hydroponic cultures enriched with Hoagland solution without phosphate. After 3 weeks, the seedlings were separated into 2 treatments: 1) added a Hoagland solution plus 1 mM Pb (NO3)2 and 2) added a Hoagland solution plus 1 mM NH4NO3. Thus, plants with Pb and without Pb were obtained to continue the experiment. After that, B. pilosa were inoculated with AMF (300-400 spores) from Bouwer: control site (Pb: 25 μg g-1) and a site with Pb (Pb: 7027 μg g-1). Each treatment had five repetitions and the plants were developed under controlled greenhouse conditions for 150 days. After that, the arbuscular mycorrhizal colonization was evaluated by roots staining, observed and counted under microscope. Arbuscular mycorrhizal fungi structures were observed in B. pilosa roots. Besides, a higher percentage of colonization (30%) was registered in plants with Pb and inoculated with AMF from the site with Pb compared to the rest of the treatments. This preliminary study show that AMF isolated from contaminated sites potentially increased the arbuscular mycorrhization in B. pilosa seedlings with Pb inside.
Fil: Becerra, Alejandra Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Menoyo, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi". Universidad Nacional de San Luis. Facultad de Ciencias Físico, Matemáticas y Naturales. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi"; Argentina
Fil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: Salazar, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology
Virtual
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Asociación Civil de Microbiología General
Materia
INOCULATION
ARBUSCULAR MYCORRHIZA
BIDENS PILOSA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/159399
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soilsBecerra, Alejandra GabrielaMenoyo, EugeniaCabello, Marta NoemíPardo, Alejandro GuillermoSalazar, María JulietaINOCULATIONARBUSCULAR MYCORRHIZABIDENS PILOSAhttps://purl.org/becyt/ford/2.8https://purl.org/becyt/ford/2The city of Córdoba is one of the most polluted cities in Argentina, with a large number of industrial plants located in urban and suburban areas. At 18 km from the capital of Córdoba is the town of Bouwer, considered one of the areas most affected by heavy metal contamination. An acid battery recycling factory dedicated to the recovery of lead (Pb) operated irregularly between 1984 and 2005. The smelter emitted Pb into the air and leaved a large amount of slag, used by neighbors to fill and level land. This caused numerous cases of Pb poisoning. Given the health risks and residence times of pollutants, it is necessary to apply remediation measures such as phytoremediation. For example the indigenous plant, B. pilosa, is a metalophytic plant species adapted to the climatic conditions and the soil of the area. The same behavior has been observed with native Arbuscular Mycorrhizal Fungi (AMF), which are important to be used as inoculants in remediation programs. Therefore, is important to select AMF species adapted to these contaminated sites. In the present work, the inoculation of B. pilosa seedlings with native AMF spores from Bouwer was evaluated. Seeds of B. pilosa collected in the field were sterilized and rinsed with sterile water. Seedlings were grown in hydroponic cultures enriched with Hoagland solution without phosphate. After 3 weeks, the seedlings were separated into 2 treatments: 1) added a Hoagland solution plus 1 mM Pb (NO3)2 and 2) added a Hoagland solution plus 1 mM NH4NO3. Thus, plants with Pb and without Pb were obtained to continue the experiment. After that, B. pilosa were inoculated with AMF (300-400 spores) from Bouwer: control site (Pb: 25 μg g-1) and a site with Pb (Pb: 7027 μg g-1). Each treatment had five repetitions and the plants were developed under controlled greenhouse conditions for 150 days. After that, the arbuscular mycorrhizal colonization was evaluated by roots staining, observed and counted under microscope. Arbuscular mycorrhizal fungi structures were observed in B. pilosa roots. Besides, a higher percentage of colonization (30%) was registered in plants with Pb and inoculated with AMF from the site with Pb compared to the rest of the treatments. This preliminary study show that AMF isolated from contaminated sites potentially increased the arbuscular mycorrhization in B. pilosa seedlings with Pb inside.Fil: Becerra, Alejandra Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Menoyo, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi". Universidad Nacional de San Luis. Facultad de Ciencias Físico, Matemáticas y Naturales. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi"; ArgentinaFil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; ArgentinaFil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; ArgentinaFil: Salazar, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaLVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General MicrobiologyVirtualArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularAsociación Civil de Microbiología GeneralTech Science Press2022info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/159399Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Virtual; Argentina; 2021; 96-960327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.techscience.com/biocell/v46nSuppl.1Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:02Zoai:ri.conicet.gov.ar:11336/159399instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:03.042CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils
title Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils
spellingShingle Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils
Becerra, Alejandra Gabriela
INOCULATION
ARBUSCULAR MYCORRHIZA
BIDENS PILOSA
title_short Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils
title_full Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils
title_fullStr Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils
title_full_unstemmed Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils
title_sort Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils
dc.creator.none.fl_str_mv Becerra, Alejandra Gabriela
Menoyo, Eugenia
Cabello, Marta Noemí
Pardo, Alejandro Guillermo
Salazar, María Julieta
author Becerra, Alejandra Gabriela
author_facet Becerra, Alejandra Gabriela
Menoyo, Eugenia
Cabello, Marta Noemí
Pardo, Alejandro Guillermo
Salazar, María Julieta
author_role author
author2 Menoyo, Eugenia
Cabello, Marta Noemí
Pardo, Alejandro Guillermo
Salazar, María Julieta
author2_role author
author
author
author
dc.subject.none.fl_str_mv INOCULATION
ARBUSCULAR MYCORRHIZA
BIDENS PILOSA
topic INOCULATION
ARBUSCULAR MYCORRHIZA
BIDENS PILOSA
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.8
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The city of Córdoba is one of the most polluted cities in Argentina, with a large number of industrial plants located in urban and suburban areas. At 18 km from the capital of Córdoba is the town of Bouwer, considered one of the areas most affected by heavy metal contamination. An acid battery recycling factory dedicated to the recovery of lead (Pb) operated irregularly between 1984 and 2005. The smelter emitted Pb into the air and leaved a large amount of slag, used by neighbors to fill and level land. This caused numerous cases of Pb poisoning. Given the health risks and residence times of pollutants, it is necessary to apply remediation measures such as phytoremediation. For example the indigenous plant, B. pilosa, is a metalophytic plant species adapted to the climatic conditions and the soil of the area. The same behavior has been observed with native Arbuscular Mycorrhizal Fungi (AMF), which are important to be used as inoculants in remediation programs. Therefore, is important to select AMF species adapted to these contaminated sites. In the present work, the inoculation of B. pilosa seedlings with native AMF spores from Bouwer was evaluated. Seeds of B. pilosa collected in the field were sterilized and rinsed with sterile water. Seedlings were grown in hydroponic cultures enriched with Hoagland solution without phosphate. After 3 weeks, the seedlings were separated into 2 treatments: 1) added a Hoagland solution plus 1 mM Pb (NO3)2 and 2) added a Hoagland solution plus 1 mM NH4NO3. Thus, plants with Pb and without Pb were obtained to continue the experiment. After that, B. pilosa were inoculated with AMF (300-400 spores) from Bouwer: control site (Pb: 25 μg g-1) and a site with Pb (Pb: 7027 μg g-1). Each treatment had five repetitions and the plants were developed under controlled greenhouse conditions for 150 days. After that, the arbuscular mycorrhizal colonization was evaluated by roots staining, observed and counted under microscope. Arbuscular mycorrhizal fungi structures were observed in B. pilosa roots. Besides, a higher percentage of colonization (30%) was registered in plants with Pb and inoculated with AMF from the site with Pb compared to the rest of the treatments. This preliminary study show that AMF isolated from contaminated sites potentially increased the arbuscular mycorrhization in B. pilosa seedlings with Pb inside.
Fil: Becerra, Alejandra Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Menoyo, Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi". Universidad Nacional de San Luis. Facultad de Ciencias Físico, Matemáticas y Naturales. Instituto de Matemática Aplicada de San Luis "Prof. Ezio Marchi"; Argentina
Fil: Cabello, Marta Noemí. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Botánica Spegazzini; Argentina
Fil: Pardo, Alejandro Guillermo. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Micología Molecular; Argentina
Fil: Salazar, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology
Virtual
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Asociación Civil de Microbiología General
description The city of Córdoba is one of the most polluted cities in Argentina, with a large number of industrial plants located in urban and suburban areas. At 18 km from the capital of Córdoba is the town of Bouwer, considered one of the areas most affected by heavy metal contamination. An acid battery recycling factory dedicated to the recovery of lead (Pb) operated irregularly between 1984 and 2005. The smelter emitted Pb into the air and leaved a large amount of slag, used by neighbors to fill and level land. This caused numerous cases of Pb poisoning. Given the health risks and residence times of pollutants, it is necessary to apply remediation measures such as phytoremediation. For example the indigenous plant, B. pilosa, is a metalophytic plant species adapted to the climatic conditions and the soil of the area. The same behavior has been observed with native Arbuscular Mycorrhizal Fungi (AMF), which are important to be used as inoculants in remediation programs. Therefore, is important to select AMF species adapted to these contaminated sites. In the present work, the inoculation of B. pilosa seedlings with native AMF spores from Bouwer was evaluated. Seeds of B. pilosa collected in the field were sterilized and rinsed with sterile water. Seedlings were grown in hydroponic cultures enriched with Hoagland solution without phosphate. After 3 weeks, the seedlings were separated into 2 treatments: 1) added a Hoagland solution plus 1 mM Pb (NO3)2 and 2) added a Hoagland solution plus 1 mM NH4NO3. Thus, plants with Pb and without Pb were obtained to continue the experiment. After that, B. pilosa were inoculated with AMF (300-400 spores) from Bouwer: control site (Pb: 25 μg g-1) and a site with Pb (Pb: 7027 μg g-1). Each treatment had five repetitions and the plants were developed under controlled greenhouse conditions for 150 days. After that, the arbuscular mycorrhizal colonization was evaluated by roots staining, observed and counted under microscope. Arbuscular mycorrhizal fungi structures were observed in B. pilosa roots. Besides, a higher percentage of colonization (30%) was registered in plants with Pb and inoculated with AMF from the site with Pb compared to the rest of the treatments. This preliminary study show that AMF isolated from contaminated sites potentially increased the arbuscular mycorrhization in B. pilosa seedlings with Pb inside.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/159399
Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Virtual; Argentina; 2021; 96-96
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/159399
identifier_str_mv Inoculation of a metalophytic plant with arbuscular mycorrhizal fungi from lead contaminated soils; LVII Annual Meeting of the Argentine Society for Biochemistry and Molecular Biology Research; XVI Annual Meeting of the Argentinean Society for General Microbiology; Virtual; Argentina; 2021; 96-96
0327-9545
1667-5746
CONICET Digital
CONICET
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language eng
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dc.publisher.none.fl_str_mv Tech Science Press
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