Biosynthesis of anti-HCV compounds using thermophilic microorganisms
- Autores
- Rivero, Cintia W.; De Benedetti, Eliana C.; Sambeth, Jorge Enrique; Lozano, Mario Enrique; Trelles, Jorge A.
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents.
Centro de Investigación y Desarrollo en Ciencias Aplicadas - Materia
-
Química
Geobacillus stearothermophilus
Nucleoside analogues
Green chemistry
Entrapment immobilization - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/128236
Ver los metadatos del registro completo
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Biosynthesis of anti-HCV compounds using thermophilic microorganismsRivero, Cintia W.De Benedetti, Eliana C.Sambeth, Jorge EnriqueLozano, Mario EnriqueTrelles, Jorge A.QuímicaGeobacillus stearothermophilusNucleoside analoguesGreen chemistryEntrapment immobilizationThis work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents.Centro de Investigación y Desarrollo en Ciencias Aplicadas2012-08-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf6059-6062http://sedici.unlp.edu.ar/handle/10915/128236enginfo:eu-repo/semantics/altIdentifier/issn/1464-3405info:eu-repo/semantics/altIdentifier/issn/0960-894xinfo:eu-repo/semantics/altIdentifier/pmid/22959520info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bmcl.2012.08.045info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:02Zoai:sedici.unlp.edu.ar:10915/128236Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:02.942SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
title |
Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
spellingShingle |
Biosynthesis of anti-HCV compounds using thermophilic microorganisms Rivero, Cintia W. Química Geobacillus stearothermophilus Nucleoside analogues Green chemistry Entrapment immobilization |
title_short |
Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
title_full |
Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
title_fullStr |
Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
title_full_unstemmed |
Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
title_sort |
Biosynthesis of anti-HCV compounds using thermophilic microorganisms |
dc.creator.none.fl_str_mv |
Rivero, Cintia W. De Benedetti, Eliana C. Sambeth, Jorge Enrique Lozano, Mario Enrique Trelles, Jorge A. |
author |
Rivero, Cintia W. |
author_facet |
Rivero, Cintia W. De Benedetti, Eliana C. Sambeth, Jorge Enrique Lozano, Mario Enrique Trelles, Jorge A. |
author_role |
author |
author2 |
De Benedetti, Eliana C. Sambeth, Jorge Enrique Lozano, Mario Enrique Trelles, Jorge A. |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Química Geobacillus stearothermophilus Nucleoside analogues Green chemistry Entrapment immobilization |
topic |
Química Geobacillus stearothermophilus Nucleoside analogues Green chemistry Entrapment immobilization |
dc.description.none.fl_txt_mv |
This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents. Centro de Investigación y Desarrollo en Ciencias Aplicadas |
description |
This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-08-21 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://sedici.unlp.edu.ar/handle/10915/128236 |
url |
http://sedici.unlp.edu.ar/handle/10915/128236 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/issn/1464-3405 info:eu-repo/semantics/altIdentifier/issn/0960-894x info:eu-repo/semantics/altIdentifier/pmid/22959520 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bmcl.2012.08.045 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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application/pdf 6059-6062 |
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Universidad Nacional de La Plata |
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SEDICI (UNLP) - Universidad Nacional de La Plata |
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alira@sedici.unlp.edu.ar |
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score |
13.070432 |