Biosynthesis of anti-HCV compounds using thermophilic microorganisms

Autores
Rivero, Cintia W.; De Benedetti, Eliana C.; Sambeth, Jorge Enrique; Lozano, Mario Enrique; Trelles, Jorge A.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents.
Centro de Investigación y Desarrollo en Ciencias Aplicadas
Materia
Química
Geobacillus stearothermophilus
Nucleoside analogues
Green chemistry
Entrapment immobilization
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/128236
Acceder
id SEDICI_9756ab9e3884792039f8d644bb18db6a
oai_identifier_str oai:sedici.unlp.edu.ar:10915/128236
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Biosynthesis of anti-HCV compounds using thermophilic microorganismsRivero, Cintia W.De Benedetti, Eliana C.Sambeth, Jorge EnriqueLozano, Mario EnriqueTrelles, Jorge A.QuímicaGeobacillus stearothermophilusNucleoside analoguesGreen chemistryEntrapment immobilizationThis work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents.Centro de Investigación y Desarrollo en Ciencias Aplicadas2012-08-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf6059-6062http://sedici.unlp.edu.ar/handle/10915/128236enginfo:eu-repo/semantics/altIdentifier/issn/1464-3405info:eu-repo/semantics/altIdentifier/issn/0960-894xinfo:eu-repo/semantics/altIdentifier/pmid/22959520info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bmcl.2012.08.045info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-05T13:10:20Zoai:sedici.unlp.edu.ar:10915/128236Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-05 13:10:21.255SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Biosynthesis of anti-HCV compounds using thermophilic microorganisms
title Biosynthesis of anti-HCV compounds using thermophilic microorganisms
spellingShingle Biosynthesis of anti-HCV compounds using thermophilic microorganisms
Rivero, Cintia W.
Química
Geobacillus stearothermophilus
Nucleoside analogues
Green chemistry
Entrapment immobilization
title_short Biosynthesis of anti-HCV compounds using thermophilic microorganisms
title_full Biosynthesis of anti-HCV compounds using thermophilic microorganisms
title_fullStr Biosynthesis of anti-HCV compounds using thermophilic microorganisms
title_full_unstemmed Biosynthesis of anti-HCV compounds using thermophilic microorganisms
title_sort Biosynthesis of anti-HCV compounds using thermophilic microorganisms
dc.creator.none.fl_str_mv Rivero, Cintia W.
De Benedetti, Eliana C.
Sambeth, Jorge Enrique
Lozano, Mario Enrique
Trelles, Jorge A.
author Rivero, Cintia W.
author_facet Rivero, Cintia W.
De Benedetti, Eliana C.
Sambeth, Jorge Enrique
Lozano, Mario Enrique
Trelles, Jorge A.
author_role author
author2 De Benedetti, Eliana C.
Sambeth, Jorge Enrique
Lozano, Mario Enrique
Trelles, Jorge A.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Química
Geobacillus stearothermophilus
Nucleoside analogues
Green chemistry
Entrapment immobilization
topic Química
Geobacillus stearothermophilus
Nucleoside analogues
Green chemistry
Entrapment immobilization
dc.description.none.fl_txt_mv This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents.
Centro de Investigación y Desarrollo en Ciencias Aplicadas
description This work describes the application of thermophilic microorganisms for obtaining 6-halogenated purine nucleosides. Biosynthesis of 6-chloropurine-2′-deoxyriboside and 6-chloropurine riboside was achieved by Geobacillus stearothermophilus CECT 43 with a conversion of 90% and 68%, respectively. Furthermore, the selected microorganism was satisfactorily stabilized by immobilization in an agarose matrix. This biocatalyst can be reused at least 70 times without significant loss of activity, obtaining 379 mg/L of 6-chloropurine-2′-deoxyriboside. The obtained compounds can be used as antiviral agents.
publishDate 2012
dc.date.none.fl_str_mv 2012-08-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/128236
url http://sedici.unlp.edu.ar/handle/10915/128236
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1464-3405
info:eu-repo/semantics/altIdentifier/issn/0960-894x
info:eu-repo/semantics/altIdentifier/pmid/22959520
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bmcl.2012.08.045
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
6059-6062
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.087074

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