Green biosynthesis of floxuridine by immobilized microorganisms

Autores
Rivero, Cintia Wanda; Britos, Claudia Noelia; Lozano, Mario Enrique; Sinisterra, Jose V.; Trelles, Jorge Abel
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This work describes an efficient, simple, and green bioprocess for obtaining 5-halogenated pyrimidine nucleosides from thymidine by transglycosylation using whole cells. Biosynthesis of 5-fluoro-2′-deoxyuridine (floxuridine) was achieved by free and immobilized Aeromonas salmonicida ATCC 27013 with an 80% and 65% conversion occurring in 1 h, respectively. The immobilized biocatalyst was stable for more than 4 months in storage conditions (4 °C) and could be reused at least 30 times without loss of its activity. This microorganism was able to biosynthesize 2.0 mg L -1 min -1 (60%) of 5-chloro-2′-deoxyuridine in 3 h. These halogenated pyrimidine 2′-deoxynucleosides are used as antitumoral agents.
Fil: Rivero, Cintia Wanda. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lozano, Mario Enrique. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sinisterra, Jose V.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Materia
5-HALOGENATED 2′-DEOXYNUCLEOSIDES
AEROMONAS SALMONICIDA
ENTRAPMENT IMMOBILIZATION
GREEN CHEMISTRY
PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/189232
Acceder
id CONICETDig_27ce4af82ebcdcf2f48248a3696bb4ce
oai_identifier_str oai:ri.conicet.gov.ar:11336/189232
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Green biosynthesis of floxuridine by immobilized microorganismsRivero, Cintia WandaBritos, Claudia NoeliaLozano, Mario EnriqueSinisterra, Jose V.Trelles, Jorge Abel5-HALOGENATED 2′-DEOXYNUCLEOSIDESAEROMONAS SALMONICIDAENTRAPMENT IMMOBILIZATIONGREEN CHEMISTRYPYRIMIDINE NUCLEOSIDE PHOSPHORYLASEhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2This work describes an efficient, simple, and green bioprocess for obtaining 5-halogenated pyrimidine nucleosides from thymidine by transglycosylation using whole cells. Biosynthesis of 5-fluoro-2′-deoxyuridine (floxuridine) was achieved by free and immobilized Aeromonas salmonicida ATCC 27013 with an 80% and 65% conversion occurring in 1 h, respectively. The immobilized biocatalyst was stable for more than 4 months in storage conditions (4 °C) and could be reused at least 30 times without loss of its activity. This microorganism was able to biosynthesize 2.0 mg L -1 min -1 (60%) of 5-chloro-2′-deoxyuridine in 3 h. These halogenated pyrimidine 2′-deoxynucleosides are used as antitumoral agents.Fil: Rivero, Cintia Wanda. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lozano, Mario Enrique. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sinisterra, Jose V.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaFil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; ArgentinaWiley Blackwell Publishing, Inc2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/189232Rivero, Cintia Wanda; Britos, Claudia Noelia; Lozano, Mario Enrique; Sinisterra, Jose V.; Trelles, Jorge Abel; Green biosynthesis of floxuridine by immobilized microorganisms; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 331; 1; 6-2012; 31-360378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/331/1/31/493950info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2012.02547.xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:16:55Zoai:ri.conicet.gov.ar:11336/189232instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:16:56.261CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Green biosynthesis of floxuridine by immobilized microorganisms
title Green biosynthesis of floxuridine by immobilized microorganisms
spellingShingle Green biosynthesis of floxuridine by immobilized microorganisms
Rivero, Cintia Wanda
5-HALOGENATED 2′-DEOXYNUCLEOSIDES
AEROMONAS SALMONICIDA
ENTRAPMENT IMMOBILIZATION
GREEN CHEMISTRY
PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE
title_short Green biosynthesis of floxuridine by immobilized microorganisms
title_full Green biosynthesis of floxuridine by immobilized microorganisms
title_fullStr Green biosynthesis of floxuridine by immobilized microorganisms
title_full_unstemmed Green biosynthesis of floxuridine by immobilized microorganisms
title_sort Green biosynthesis of floxuridine by immobilized microorganisms
dc.creator.none.fl_str_mv Rivero, Cintia Wanda
Britos, Claudia Noelia
Lozano, Mario Enrique
Sinisterra, Jose V.
Trelles, Jorge Abel
author Rivero, Cintia Wanda
author_facet Rivero, Cintia Wanda
Britos, Claudia Noelia
Lozano, Mario Enrique
Sinisterra, Jose V.
Trelles, Jorge Abel
author_role author
author2 Britos, Claudia Noelia
Lozano, Mario Enrique
Sinisterra, Jose V.
Trelles, Jorge Abel
author2_role author
author
author
author
dc.subject.none.fl_str_mv 5-HALOGENATED 2′-DEOXYNUCLEOSIDES
AEROMONAS SALMONICIDA
ENTRAPMENT IMMOBILIZATION
GREEN CHEMISTRY
PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE
topic 5-HALOGENATED 2′-DEOXYNUCLEOSIDES
AEROMONAS SALMONICIDA
ENTRAPMENT IMMOBILIZATION
GREEN CHEMISTRY
PYRIMIDINE NUCLEOSIDE PHOSPHORYLASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv This work describes an efficient, simple, and green bioprocess for obtaining 5-halogenated pyrimidine nucleosides from thymidine by transglycosylation using whole cells. Biosynthesis of 5-fluoro-2′-deoxyuridine (floxuridine) was achieved by free and immobilized Aeromonas salmonicida ATCC 27013 with an 80% and 65% conversion occurring in 1 h, respectively. The immobilized biocatalyst was stable for more than 4 months in storage conditions (4 °C) and could be reused at least 30 times without loss of its activity. This microorganism was able to biosynthesize 2.0 mg L -1 min -1 (60%) of 5-chloro-2′-deoxyuridine in 3 h. These halogenated pyrimidine 2′-deoxynucleosides are used as antitumoral agents.
Fil: Rivero, Cintia Wanda. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Britos, Claudia Noelia. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lozano, Mario Enrique. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sinisterra, Jose V.. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
Fil: Trelles, Jorge Abel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Investigación en Biotecnología Sustentable; Argentina
description This work describes an efficient, simple, and green bioprocess for obtaining 5-halogenated pyrimidine nucleosides from thymidine by transglycosylation using whole cells. Biosynthesis of 5-fluoro-2′-deoxyuridine (floxuridine) was achieved by free and immobilized Aeromonas salmonicida ATCC 27013 with an 80% and 65% conversion occurring in 1 h, respectively. The immobilized biocatalyst was stable for more than 4 months in storage conditions (4 °C) and could be reused at least 30 times without loss of its activity. This microorganism was able to biosynthesize 2.0 mg L -1 min -1 (60%) of 5-chloro-2′-deoxyuridine in 3 h. These halogenated pyrimidine 2′-deoxynucleosides are used as antitumoral agents.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/189232
Rivero, Cintia Wanda; Britos, Claudia Noelia; Lozano, Mario Enrique; Sinisterra, Jose V.; Trelles, Jorge Abel; Green biosynthesis of floxuridine by immobilized microorganisms; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 331; 1; 6-2012; 31-36
0378-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/189232
identifier_str_mv Rivero, Cintia Wanda; Britos, Claudia Noelia; Lozano, Mario Enrique; Sinisterra, Jose V.; Trelles, Jorge Abel; Green biosynthesis of floxuridine by immobilized microorganisms; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 331; 1; 6-2012; 31-36
0378-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/331/1/31/493950
info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2012.02547.x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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