Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity

Autores
Carvalho, Eneas; Barbosa, Angela S.; Gómez, Ricardo Martín; Cianciarullo, Aurora M.; Hauk, Pricila; Abreu, Patrícia A.; Fiorini, Leonardo C.; Oliveira, Maria L. S.; Romero, Eliete C.; Gonçales, Amane P.; Morais, Zenaide M.; Vasconcellos, Sílvio A.; Ho, Paulo L.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The role of TlyA, TlyB and TlyC proteins in the biology of Leptospira is still uncertain. Although these proteins have been considered as putative hemolysins, we demonstrate that leptospiral recombinant TlyB and TlyC do not possess hemolytic activity. However, further experiments showed that TlyC is a surface-exposed protein that seems to bind to laminin, collagen IV and fibronectin. The expression of both proteins was detected both in vitro and in vivo. Our findings suggest that TlyB and TlyC are not directly involved in hemolysis, and that TlyC may contribute to Leptospira binding to extracellular matrix (ECM) during host infection.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
Materia
Ciencias Exactas
Biología
TlyB
TlyC
Hemolysis
Extracellular matrix
Leptospira
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/127120
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/127120
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activityCarvalho, EneasBarbosa, Angela S.Gómez, Ricardo MartínCianciarullo, Aurora M.Hauk, PricilaAbreu, Patrícia A.Fiorini, Leonardo C.Oliveira, Maria L. S.Romero, Eliete C.Gonçales, Amane P.Morais, Zenaide M.Vasconcellos, Sílvio A.Ho, Paulo L.Ciencias ExactasBiologíaTlyBTlyCHemolysisExtracellular matrixLeptospiraThe role of TlyA, TlyB and TlyC proteins in the biology of <i>Leptospira</i> is still uncertain. Although these proteins have been considered as putative hemolysins, we demonstrate that leptospiral recombinant TlyB and TlyC do not possess hemolytic activity. However, further experiments showed that TlyC is a surface-exposed protein that seems to bind to laminin, collagen IV and fibronectin. The expression of both proteins was detected both in vitro and in vivo. Our findings suggest that TlyB and TlyC are not directly involved in hemolysis, and that TlyC may contribute to <i>Leptospira</i> binding to extracellular matrix (ECM) during host infection.Facultad de Ciencias ExactasInstituto de Biotecnologia y Biologia Molecular2009-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1381-1385http://sedici.unlp.edu.ar/handle/10915/127120enginfo:eu-repo/semantics/altIdentifier/issn/1873-3468info:eu-repo/semantics/altIdentifier/issn/0014-5793info:eu-repo/semantics/altIdentifier/pmid/19328790info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2009.03.050info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:30:42Zoai:sedici.unlp.edu.ar:10915/127120Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:30:43.12SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
spellingShingle Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
Carvalho, Eneas
Ciencias Exactas
Biología
TlyB
TlyC
Hemolysis
Extracellular matrix
Leptospira
title_short Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title_full Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title_fullStr Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title_full_unstemmed Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
title_sort Leptospiral TlyC is an extracellular matrix-binding protein and does not present hemolysin activity
dc.creator.none.fl_str_mv Carvalho, Eneas
Barbosa, Angela S.
Gómez, Ricardo Martín
Cianciarullo, Aurora M.
Hauk, Pricila
Abreu, Patrícia A.
Fiorini, Leonardo C.
Oliveira, Maria L. S.
Romero, Eliete C.
Gonçales, Amane P.
Morais, Zenaide M.
Vasconcellos, Sílvio A.
Ho, Paulo L.
author Carvalho, Eneas
author_facet Carvalho, Eneas
Barbosa, Angela S.
Gómez, Ricardo Martín
Cianciarullo, Aurora M.
Hauk, Pricila
Abreu, Patrícia A.
Fiorini, Leonardo C.
Oliveira, Maria L. S.
Romero, Eliete C.
Gonçales, Amane P.
Morais, Zenaide M.
Vasconcellos, Sílvio A.
Ho, Paulo L.
author_role author
author2 Barbosa, Angela S.
Gómez, Ricardo Martín
Cianciarullo, Aurora M.
Hauk, Pricila
Abreu, Patrícia A.
Fiorini, Leonardo C.
Oliveira, Maria L. S.
Romero, Eliete C.
Gonçales, Amane P.
Morais, Zenaide M.
Vasconcellos, Sílvio A.
Ho, Paulo L.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Biología
TlyB
TlyC
Hemolysis
Extracellular matrix
Leptospira
topic Ciencias Exactas
Biología
TlyB
TlyC
Hemolysis
Extracellular matrix
Leptospira
dc.description.none.fl_txt_mv The role of TlyA, TlyB and TlyC proteins in the biology of <i>Leptospira</i> is still uncertain. Although these proteins have been considered as putative hemolysins, we demonstrate that leptospiral recombinant TlyB and TlyC do not possess hemolytic activity. However, further experiments showed that TlyC is a surface-exposed protein that seems to bind to laminin, collagen IV and fibronectin. The expression of both proteins was detected both in vitro and in vivo. Our findings suggest that TlyB and TlyC are not directly involved in hemolysis, and that TlyC may contribute to <i>Leptospira</i> binding to extracellular matrix (ECM) during host infection.
Facultad de Ciencias Exactas
Instituto de Biotecnologia y Biologia Molecular
description The role of TlyA, TlyB and TlyC proteins in the biology of <i>Leptospira</i> is still uncertain. Although these proteins have been considered as putative hemolysins, we demonstrate that leptospiral recombinant TlyB and TlyC do not possess hemolytic activity. However, further experiments showed that TlyC is a surface-exposed protein that seems to bind to laminin, collagen IV and fibronectin. The expression of both proteins was detected both in vitro and in vivo. Our findings suggest that TlyB and TlyC are not directly involved in hemolysis, and that TlyC may contribute to <i>Leptospira</i> binding to extracellular matrix (ECM) during host infection.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/127120
url http://sedici.unlp.edu.ar/handle/10915/127120
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1873-3468
info:eu-repo/semantics/altIdentifier/issn/0014-5793
info:eu-repo/semantics/altIdentifier/pmid/19328790
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.febslet.2009.03.050
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1381-1385
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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