Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia

Autores
Samouillan, Valerie; Garcia, Eduardo; Benitez Amaro, Aleyda; La Chica Lhoëst, Maria Teresa; Dandurand, Jany; Actis Dato, Virginia; Guerra, Jose Maria; Escolà Gil, Joan Carles; Chiabrando, Gustavo Alberto; Enrich, Carlos; Llorente-Cortes, Vicenta
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The accumulation of lipids in cardiomyocytes contributes to cardiac dysfunction. The specific blockage of cardiomyocyte cholesteryl ester (CE) loading by antibodies (Abs) against the P3 sequence (Gly1127–Cys1140) of the LRP1 receptor improves cardiac insulin sensitivity. The impact of anti-P3 Abs on high-fat diet (HFD)-induced cardiac extracellular matrix (ECM) biophysical alterations was analyzed. Both IrP (without Abs) and P3-immunized rabbits (with Abs) were randomized into groups fed either HFD or a standard chow diet. Cardiac lipids, proteins, and carbohydrates were characterized by Fourier transform infrared spectroscopy in the attenuated total reflectance mode. The hydric organization and physical structure were determined by differential scanning calorimetry. HFD increased the levels of esterified lipids, collagen, and α-helical structures and upregulated fibrosis, bound water, and ECM plasticization in the heart. The inhibitory effect of anti-P3 Abs on cardiac CE accumulation was sufficient to reduce the collagen-filled extracellular space, the level of fibrosis, and the amount of bound water but did not counteract ECM plasticization in the heart of hypercholesterolemic rabbits.
Fil: Samouillan, Valerie. Université de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Garcia, Eduardo. Universitat Autònoma de Barcelona; España. Instituto de Investigaciones Biomedicas de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: Benitez Amaro, Aleyda. Universitat Autònoma de Barcelona; España. Instituto de Investigaciones Biomedicas de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: La Chica Lhoëst, Maria Teresa. Universitat Autònoma de Barcelona; España. Université Paul Sabatier; Francia. Instituto de Investigaciones Biomedicas de Barcelona; España
Fil: Dandurand, Jany. Université de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Actis Dato, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Guerra, Jose Maria. Universitat Autònoma de Barcelona; España
Fil: Escolà Gil, Joan Carles. Institut Dinvestigació Biomèdica Sant Pau; España. Metabolic Basis of Cardiovascular Risk, Biomedical Research Institute Sant Pau; España
Fil: Chiabrando, Gustavo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Enrich, Carlos. Universidad de Barcelona; España
Fil: Llorente-Cortes, Vicenta. Centro de Investigación En Red En Enfermedades Cardiovasculares; España. Instituto de Investigaciones Biomedicas de Barcelona; España. Universitat Autònoma de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Materia
EXTRACELLULAR MATRIX
CHOLESTEROL
IMMUNOTHERAPY
HEART
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/226896
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/226896
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by HypercholesterolemiaSamouillan, ValerieGarcia, EduardoBenitez Amaro, AleydaLa Chica Lhoëst, Maria TeresaDandurand, JanyActis Dato, VirginiaGuerra, Jose MariaEscolà Gil, Joan CarlesChiabrando, Gustavo AlbertoEnrich, CarlosLlorente-Cortes, VicentaEXTRACELLULAR MATRIXCHOLESTEROLIMMUNOTHERAPYHEARThttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The accumulation of lipids in cardiomyocytes contributes to cardiac dysfunction. The specific blockage of cardiomyocyte cholesteryl ester (CE) loading by antibodies (Abs) against the P3 sequence (Gly1127–Cys1140) of the LRP1 receptor improves cardiac insulin sensitivity. The impact of anti-P3 Abs on high-fat diet (HFD)-induced cardiac extracellular matrix (ECM) biophysical alterations was analyzed. Both IrP (without Abs) and P3-immunized rabbits (with Abs) were randomized into groups fed either HFD or a standard chow diet. Cardiac lipids, proteins, and carbohydrates were characterized by Fourier transform infrared spectroscopy in the attenuated total reflectance mode. The hydric organization and physical structure were determined by differential scanning calorimetry. HFD increased the levels of esterified lipids, collagen, and α-helical structures and upregulated fibrosis, bound water, and ECM plasticization in the heart. The inhibitory effect of anti-P3 Abs on cardiac CE accumulation was sufficient to reduce the collagen-filled extracellular space, the level of fibrosis, and the amount of bound water but did not counteract ECM plasticization in the heart of hypercholesterolemic rabbits.Fil: Samouillan, Valerie. Université de Toulouse; Francia. Université Paul Sabatier; FranciaFil: Garcia, Eduardo. Universitat Autònoma de Barcelona; España. Instituto de Investigaciones Biomedicas de Barcelona; España. Consejo Superior de Investigaciones Científicas; EspañaFil: Benitez Amaro, Aleyda. Universitat Autònoma de Barcelona; España. Instituto de Investigaciones Biomedicas de Barcelona; España. Consejo Superior de Investigaciones Científicas; EspañaFil: La Chica Lhoëst, Maria Teresa. Universitat Autònoma de Barcelona; España. Université Paul Sabatier; Francia. Instituto de Investigaciones Biomedicas de Barcelona; EspañaFil: Dandurand, Jany. Université de Toulouse; Francia. Université Paul Sabatier; FranciaFil: Actis Dato, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Guerra, Jose Maria. Universitat Autònoma de Barcelona; EspañaFil: Escolà Gil, Joan Carles. Institut Dinvestigació Biomèdica Sant Pau; España. Metabolic Basis of Cardiovascular Risk, Biomedical Research Institute Sant Pau; EspañaFil: Chiabrando, Gustavo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Enrich, Carlos. Universidad de Barcelona; EspañaFil: Llorente-Cortes, Vicenta. Centro de Investigación En Red En Enfermedades Cardiovasculares; España. Instituto de Investigaciones Biomedicas de Barcelona; España. Universitat Autònoma de Barcelona; España. Consejo Superior de Investigaciones Científicas; EspañaAmerican Chemical Society2023-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/226896Samouillan, Valerie; Garcia, Eduardo; Benitez Amaro, Aleyda; La Chica Lhoëst, Maria Teresa; Dandurand, Jany; et al.; Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia; American Chemical Society; Journal of Medicinal Chemistry; 66; 9; 4-2023; 6251-62620022-2623CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.jmedchem.2c02103info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jmedchem.2c02103info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:39:55Zoai:ri.conicet.gov.ar:11336/226896instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:39:56.189CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia
title Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia
spellingShingle Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia
Samouillan, Valerie
EXTRACELLULAR MATRIX
CHOLESTEROL
IMMUNOTHERAPY
HEART
title_short Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia
title_full Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia
title_fullStr Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia
title_full_unstemmed Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia
title_sort Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia
dc.creator.none.fl_str_mv Samouillan, Valerie
Garcia, Eduardo
Benitez Amaro, Aleyda
La Chica Lhoëst, Maria Teresa
Dandurand, Jany
Actis Dato, Virginia
Guerra, Jose Maria
Escolà Gil, Joan Carles
Chiabrando, Gustavo Alberto
Enrich, Carlos
Llorente-Cortes, Vicenta
author Samouillan, Valerie
author_facet Samouillan, Valerie
Garcia, Eduardo
Benitez Amaro, Aleyda
La Chica Lhoëst, Maria Teresa
Dandurand, Jany
Actis Dato, Virginia
Guerra, Jose Maria
Escolà Gil, Joan Carles
Chiabrando, Gustavo Alberto
Enrich, Carlos
Llorente-Cortes, Vicenta
author_role author
author2 Garcia, Eduardo
Benitez Amaro, Aleyda
La Chica Lhoëst, Maria Teresa
Dandurand, Jany
Actis Dato, Virginia
Guerra, Jose Maria
Escolà Gil, Joan Carles
Chiabrando, Gustavo Alberto
Enrich, Carlos
Llorente-Cortes, Vicenta
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv EXTRACELLULAR MATRIX
CHOLESTEROL
IMMUNOTHERAPY
HEART
topic EXTRACELLULAR MATRIX
CHOLESTEROL
IMMUNOTHERAPY
HEART
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The accumulation of lipids in cardiomyocytes contributes to cardiac dysfunction. The specific blockage of cardiomyocyte cholesteryl ester (CE) loading by antibodies (Abs) against the P3 sequence (Gly1127–Cys1140) of the LRP1 receptor improves cardiac insulin sensitivity. The impact of anti-P3 Abs on high-fat diet (HFD)-induced cardiac extracellular matrix (ECM) biophysical alterations was analyzed. Both IrP (without Abs) and P3-immunized rabbits (with Abs) were randomized into groups fed either HFD or a standard chow diet. Cardiac lipids, proteins, and carbohydrates were characterized by Fourier transform infrared spectroscopy in the attenuated total reflectance mode. The hydric organization and physical structure were determined by differential scanning calorimetry. HFD increased the levels of esterified lipids, collagen, and α-helical structures and upregulated fibrosis, bound water, and ECM plasticization in the heart. The inhibitory effect of anti-P3 Abs on cardiac CE accumulation was sufficient to reduce the collagen-filled extracellular space, the level of fibrosis, and the amount of bound water but did not counteract ECM plasticization in the heart of hypercholesterolemic rabbits.
Fil: Samouillan, Valerie. Université de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Garcia, Eduardo. Universitat Autònoma de Barcelona; España. Instituto de Investigaciones Biomedicas de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: Benitez Amaro, Aleyda. Universitat Autònoma de Barcelona; España. Instituto de Investigaciones Biomedicas de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
Fil: La Chica Lhoëst, Maria Teresa. Universitat Autònoma de Barcelona; España. Université Paul Sabatier; Francia. Instituto de Investigaciones Biomedicas de Barcelona; España
Fil: Dandurand, Jany. Université de Toulouse; Francia. Université Paul Sabatier; Francia
Fil: Actis Dato, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Córdoba; Argentina
Fil: Guerra, Jose Maria. Universitat Autònoma de Barcelona; España
Fil: Escolà Gil, Joan Carles. Institut Dinvestigació Biomèdica Sant Pau; España. Metabolic Basis of Cardiovascular Risk, Biomedical Research Institute Sant Pau; España
Fil: Chiabrando, Gustavo Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Enrich, Carlos. Universidad de Barcelona; España
Fil: Llorente-Cortes, Vicenta. Centro de Investigación En Red En Enfermedades Cardiovasculares; España. Instituto de Investigaciones Biomedicas de Barcelona; España. Universitat Autònoma de Barcelona; España. Consejo Superior de Investigaciones Científicas; España
description The accumulation of lipids in cardiomyocytes contributes to cardiac dysfunction. The specific blockage of cardiomyocyte cholesteryl ester (CE) loading by antibodies (Abs) against the P3 sequence (Gly1127–Cys1140) of the LRP1 receptor improves cardiac insulin sensitivity. The impact of anti-P3 Abs on high-fat diet (HFD)-induced cardiac extracellular matrix (ECM) biophysical alterations was analyzed. Both IrP (without Abs) and P3-immunized rabbits (with Abs) were randomized into groups fed either HFD or a standard chow diet. Cardiac lipids, proteins, and carbohydrates were characterized by Fourier transform infrared spectroscopy in the attenuated total reflectance mode. The hydric organization and physical structure were determined by differential scanning calorimetry. HFD increased the levels of esterified lipids, collagen, and α-helical structures and upregulated fibrosis, bound water, and ECM plasticization in the heart. The inhibitory effect of anti-P3 Abs on cardiac CE accumulation was sufficient to reduce the collagen-filled extracellular space, the level of fibrosis, and the amount of bound water but did not counteract ECM plasticization in the heart of hypercholesterolemic rabbits.
publishDate 2023
dc.date.none.fl_str_mv 2023-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/226896
Samouillan, Valerie; Garcia, Eduardo; Benitez Amaro, Aleyda; La Chica Lhoëst, Maria Teresa; Dandurand, Jany; et al.; Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia; American Chemical Society; Journal of Medicinal Chemistry; 66; 9; 4-2023; 6251-6262
0022-2623
CONICET Digital
CONICET
url http://hdl.handle.net/11336/226896
identifier_str_mv Samouillan, Valerie; Garcia, Eduardo; Benitez Amaro, Aleyda; La Chica Lhoëst, Maria Teresa; Dandurand, Jany; et al.; Inhibitory Effects of LRP1-Based Immunotherapy on Cardiac Extracellular Matrix Biophysical Alterations Induced by Hypercholesterolemia; American Chemical Society; Journal of Medicinal Chemistry; 66; 9; 4-2023; 6251-6262
0022-2623
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acs.jmedchem.2c02103
info:eu-repo/semantics/altIdentifier/doi/10.1021/acs.jmedchem.2c02103
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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