Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells

Autores
Atzingen, Marina V.; Gomez, Ricardo Martin; Schattner, Mirta Ana; Prêtre, Gabriela; Goncalez, Amane P.; de Morais, Zenaide M.; Vasconcellos, Silvio A.; Nascimento. Ana L. T. O.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The study of a predicted outer membrane leptospiral protein encoded by the gene LIC12690 in mediating the adhesion process. The gene was cloned and expressed in Escherichia coli BL21 (SI) strain by using the expression vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and used to assess its ability to activate human umbilical vein endothelial cells (HUVECs).
Fil: Atzingen, Marina V.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Prêtre, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Goncalez, Amane P.. Universidade de Sao Paulo; Brasil
Fil: de Morais, Zenaide M.. Universidade de Sao Paulo; Brasil
Fil: Vasconcellos, Silvio A.. Universidade de Sao Paulo; Brasil
Fil: Nascimento. Ana L. T. O.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; Brasil
Materia
Endothelial Cells
Leptospira
Adhesion Molecules
E-Selectin
Gene Expression
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55091
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/55091
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cellsAtzingen, Marina V.Gomez, Ricardo MartinSchattner, Mirta AnaPrêtre, GabrielaGoncalez, Amane P.de Morais, Zenaide M.Vasconcellos, Silvio A.Nascimento. Ana L. T. O.Endothelial CellsLeptospiraAdhesion MoleculesE-SelectinGene Expressionhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The study of a predicted outer membrane leptospiral protein encoded by the gene LIC12690 in mediating the adhesion process. The gene was cloned and expressed in Escherichia coli BL21 (SI) strain by using the expression vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and used to assess its ability to activate human umbilical vein endothelial cells (HUVECs).Fil: Atzingen, Marina V.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; BrasilFil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Prêtre, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; ArgentinaFil: Goncalez, Amane P.. Universidade de Sao Paulo; BrasilFil: de Morais, Zenaide M.. Universidade de Sao Paulo; BrasilFil: Vasconcellos, Silvio A.. Universidade de Sao Paulo; BrasilFil: Nascimento. Ana L. T. O.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; BrasilW B Saunders Co Ltd2009-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55091Atzingen, Marina V.; Gomez, Ricardo Martin; Schattner, Mirta Ana; Prêtre, Gabriela; Goncalez, Amane P.; et al.; Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells; W B Saunders Co Ltd; The Journal Of Infection.; 59; 4; 10-2009; 264-2760163-44531532-2742CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.journalofinfection.com/article/S0163-4453(09)00189-3/fulltextinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.jinf.2009.07.010info:eu-repo/semantics/altIdentifier/pmid/19665803info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:23:00Zoai:ri.conicet.gov.ar:11336/55091instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:23:00.351CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells
title Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells
spellingShingle Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells
Atzingen, Marina V.
Endothelial Cells
Leptospira
Adhesion Molecules
E-Selectin
Gene Expression
title_short Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells
title_full Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells
title_fullStr Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells
title_full_unstemmed Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells
title_sort Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells
dc.creator.none.fl_str_mv Atzingen, Marina V.
Gomez, Ricardo Martin
Schattner, Mirta Ana
Prêtre, Gabriela
Goncalez, Amane P.
de Morais, Zenaide M.
Vasconcellos, Silvio A.
Nascimento. Ana L. T. O.
author Atzingen, Marina V.
author_facet Atzingen, Marina V.
Gomez, Ricardo Martin
Schattner, Mirta Ana
Prêtre, Gabriela
Goncalez, Amane P.
de Morais, Zenaide M.
Vasconcellos, Silvio A.
Nascimento. Ana L. T. O.
author_role author
author2 Gomez, Ricardo Martin
Schattner, Mirta Ana
Prêtre, Gabriela
Goncalez, Amane P.
de Morais, Zenaide M.
Vasconcellos, Silvio A.
Nascimento. Ana L. T. O.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Endothelial Cells
Leptospira
Adhesion Molecules
E-Selectin
Gene Expression
topic Endothelial Cells
Leptospira
Adhesion Molecules
E-Selectin
Gene Expression
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The study of a predicted outer membrane leptospiral protein encoded by the gene LIC12690 in mediating the adhesion process. The gene was cloned and expressed in Escherichia coli BL21 (SI) strain by using the expression vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and used to assess its ability to activate human umbilical vein endothelial cells (HUVECs).
Fil: Atzingen, Marina V.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil
Fil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Prêtre, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina
Fil: Goncalez, Amane P.. Universidade de Sao Paulo; Brasil
Fil: de Morais, Zenaide M.. Universidade de Sao Paulo; Brasil
Fil: Vasconcellos, Silvio A.. Universidade de Sao Paulo; Brasil
Fil: Nascimento. Ana L. T. O.. Governo do Estado de Sao Paulo. Secretaria da Saude. Instituto Butantan; Brasil. Universidade de Sao Paulo; Brasil
description The study of a predicted outer membrane leptospiral protein encoded by the gene LIC12690 in mediating the adhesion process. The gene was cloned and expressed in Escherichia coli BL21 (SI) strain by using the expression vector pAE. The recombinant protein tagged with N-terminal hexahistidine was purified by metal-charged chromatography and used to assess its ability to activate human umbilical vein endothelial cells (HUVECs).
publishDate 2009
dc.date.none.fl_str_mv 2009-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55091
Atzingen, Marina V.; Gomez, Ricardo Martin; Schattner, Mirta Ana; Prêtre, Gabriela; Goncalez, Amane P.; et al.; Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells; W B Saunders Co Ltd; The Journal Of Infection.; 59; 4; 10-2009; 264-276
0163-4453
1532-2742
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55091
identifier_str_mv Atzingen, Marina V.; Gomez, Ricardo Martin; Schattner, Mirta Ana; Prêtre, Gabriela; Goncalez, Amane P.; et al.; Lp95, a novel leptospiral protein that binds extracellular matrix components and activates e-selectin on endothelial cells; W B Saunders Co Ltd; The Journal Of Infection.; 59; 4; 10-2009; 264-276
0163-4453
1532-2742
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.journalofinfection.com/article/S0163-4453(09)00189-3/fulltext
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jinf.2009.07.010
info:eu-repo/semantics/altIdentifier/pmid/19665803
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv W B Saunders Co Ltd
publisher.none.fl_str_mv W B Saunders Co Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082633507799040
score 13.22299

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