First Study of Different Insect Cells to Triatoma Virus Infection

Autores
Susevich, María Laura; Martí, Gerardo Aníbal; Metz, Germán Ernesto; Echeverría, María Gabriela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas disease, which is endemic in Latin America but also expanding its worldwide distribution. To this end, we attempted virus replication in Diptera, Aedes albopictus (clone C6/36) and Lepidoptera Spodoptera frugiperda (SF9, SF21) and High Five (H5) cell lines. The methodologies used were transfection process, direct inoculation (purified virus), and inoculation of purified virus with trypsin. Results were confirmed by SDS-PAGE, Western blotting, RT-PCR, electron microscopy, and immunofluorescence. According to the results obtained, further analysis of susceptibility/infection of H5 cells to TrV required to be studied.
Centro de Estudios Parasitológicos y de Vectores
Facultad de Ciencias Veterinarias
Materia
Biología
Insect Cell Line
Paralysis Virus
Black Queen Cell Virus
Blind Passage
Taura Syndrome Virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/135174
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/135174
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling First Study of Different Insect Cells to Triatoma Virus InfectionSusevich, María LauraMartí, Gerardo AníbalMetz, Germán ErnestoEcheverría, María GabrielaBiologíaInsect Cell LineParalysis VirusBlack Queen Cell VirusBlind PassageTaura Syndrome VirusThe use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas disease, which is endemic in Latin America but also expanding its worldwide distribution. To this end, we attempted virus replication in Diptera, Aedes albopictus (clone C6/36) and Lepidoptera Spodoptera frugiperda (SF9, SF21) and High Five (H5) cell lines. The methodologies used were transfection process, direct inoculation (purified virus), and inoculation of purified virus with trypsin. Results were confirmed by SDS-PAGE, Western blotting, RT-PCR, electron microscopy, and immunofluorescence. According to the results obtained, further analysis of susceptibility/infection of H5 cells to TrV required to be studied.Centro de Estudios Parasitológicos y de VectoresFacultad de Ciencias Veterinarias2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf470-475http://sedici.unlp.edu.ar/handle/10915/135174enginfo:eu-repo/semantics/altIdentifier/issn/1432-0991info:eu-repo/semantics/altIdentifier/issn/0343-8651info:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-014-0746-xinfo:eu-repo/semantics/altIdentifier/pmid/25481388info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:31:52Zoai:sedici.unlp.edu.ar:10915/135174Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:31:52.28SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv First Study of Different Insect Cells to Triatoma Virus Infection
title First Study of Different Insect Cells to Triatoma Virus Infection
spellingShingle First Study of Different Insect Cells to Triatoma Virus Infection
Susevich, María Laura
Biología
Insect Cell Line
Paralysis Virus
Black Queen Cell Virus
Blind Passage
Taura Syndrome Virus
title_short First Study of Different Insect Cells to Triatoma Virus Infection
title_full First Study of Different Insect Cells to Triatoma Virus Infection
title_fullStr First Study of Different Insect Cells to Triatoma Virus Infection
title_full_unstemmed First Study of Different Insect Cells to Triatoma Virus Infection
title_sort First Study of Different Insect Cells to Triatoma Virus Infection
dc.creator.none.fl_str_mv Susevich, María Laura
Martí, Gerardo Aníbal
Metz, Germán Ernesto
Echeverría, María Gabriela
author Susevich, María Laura
author_facet Susevich, María Laura
Martí, Gerardo Aníbal
Metz, Germán Ernesto
Echeverría, María Gabriela
author_role author
author2 Martí, Gerardo Aníbal
Metz, Germán Ernesto
Echeverría, María Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv Biología
Insect Cell Line
Paralysis Virus
Black Queen Cell Virus
Blind Passage
Taura Syndrome Virus
topic Biología
Insect Cell Line
Paralysis Virus
Black Queen Cell Virus
Blind Passage
Taura Syndrome Virus
dc.description.none.fl_txt_mv The use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas disease, which is endemic in Latin America but also expanding its worldwide distribution. To this end, we attempted virus replication in Diptera, Aedes albopictus (clone C6/36) and Lepidoptera Spodoptera frugiperda (SF9, SF21) and High Five (H5) cell lines. The methodologies used were transfection process, direct inoculation (purified virus), and inoculation of purified virus with trypsin. Results were confirmed by SDS-PAGE, Western blotting, RT-PCR, electron microscopy, and immunofluorescence. According to the results obtained, further analysis of susceptibility/infection of H5 cells to TrV required to be studied.
Centro de Estudios Parasitológicos y de Vectores
Facultad de Ciencias Veterinarias
description The use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas disease, which is endemic in Latin America but also expanding its worldwide distribution. To this end, we attempted virus replication in Diptera, Aedes albopictus (clone C6/36) and Lepidoptera Spodoptera frugiperda (SF9, SF21) and High Five (H5) cell lines. The methodologies used were transfection process, direct inoculation (purified virus), and inoculation of purified virus with trypsin. Results were confirmed by SDS-PAGE, Western blotting, RT-PCR, electron microscopy, and immunofluorescence. According to the results obtained, further analysis of susceptibility/infection of H5 cells to TrV required to be studied.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/135174
url http://sedici.unlp.edu.ar/handle/10915/135174
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1432-0991
info:eu-repo/semantics/altIdentifier/issn/0343-8651
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-014-0746-x
info:eu-repo/semantics/altIdentifier/pmid/25481388
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
470-475
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.070432

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