Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan

Autores
Talarico, L.B.; Noseda, M.D.; Ducatti, D.R.B.; Duarte, M.E.R.; Damonte, E.B.
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM.
Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Noseda, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fuente
J. Gen. Virol. 2011;92(6):1332-1342
Materia
carrageenan
heparan sulfate
animal cell
antiviral activity
article
cell proliferation
controlled study
dengue
Dengue virus
insect cell
mammal cell
mosquito
nonhuman
priority journal
protein synthesis
Vero cell
virus inhibition
Animals
Carrageenan
Cell Line
Cercopithecus aethiops
Culicidae
Dengue
Dengue Virus
Vero Cells
Virus Replication
Dengue virus
Mammalia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
paperaa:paper_00221317_v92_n6_p1332_Talarico

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oai_identifier_str paperaa:paper_00221317_v92_n6_p1332_Talarico
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenanTalarico, L.B.Noseda, M.D.Ducatti, D.R.B.Duarte, M.E.R.Damonte, E.B.carrageenanheparan sulfateanimal cellantiviral activityarticlecell proliferationcontrolled studydengueDengue virusinsect cellmammal cellmosquitononhumanpriority journalprotein synthesisVero cellvirus inhibitionAnimalsCarrageenanCell LineCercopithecus aethiopsCulicidaeDengueDengue VirusVero CellsVirus ReplicationDengue virusMammaliaThe antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM.Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Noseda, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.2011info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_TalaricoJ. Gen. Virol. 2011;92(6):1332-1342reponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCENenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/2.5/ar2025-09-29T13:43:03Zpaperaa:paper_00221317_v92_n6_p1332_TalaricoInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-09-29 13:43:04.787Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
spellingShingle Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
Talarico, L.B.
carrageenan
heparan sulfate
animal cell
antiviral activity
article
cell proliferation
controlled study
dengue
Dengue virus
insect cell
mammal cell
mosquito
nonhuman
priority journal
protein synthesis
Vero cell
virus inhibition
Animals
Carrageenan
Cell Line
Cercopithecus aethiops
Culicidae
Dengue
Dengue Virus
Vero Cells
Virus Replication
Dengue virus
Mammalia
title_short Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title_full Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title_fullStr Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title_full_unstemmed Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
title_sort Differential inhibition of dengue virus infection in mammalian and mosquito cells by iota-carrageenan
dc.creator.none.fl_str_mv Talarico, L.B.
Noseda, M.D.
Ducatti, D.R.B.
Duarte, M.E.R.
Damonte, E.B.
author Talarico, L.B.
author_facet Talarico, L.B.
Noseda, M.D.
Ducatti, D.R.B.
Duarte, M.E.R.
Damonte, E.B.
author_role author
author2 Noseda, M.D.
Ducatti, D.R.B.
Duarte, M.E.R.
Damonte, E.B.
author2_role author
author
author
author
dc.subject.none.fl_str_mv carrageenan
heparan sulfate
animal cell
antiviral activity
article
cell proliferation
controlled study
dengue
Dengue virus
insect cell
mammal cell
mosquito
nonhuman
priority journal
protein synthesis
Vero cell
virus inhibition
Animals
Carrageenan
Cell Line
Cercopithecus aethiops
Culicidae
Dengue
Dengue Virus
Vero Cells
Virus Replication
Dengue virus
Mammalia
topic carrageenan
heparan sulfate
animal cell
antiviral activity
article
cell proliferation
controlled study
dengue
Dengue virus
insect cell
mammal cell
mosquito
nonhuman
priority journal
protein synthesis
Vero cell
virus inhibition
Animals
Carrageenan
Cell Line
Cercopithecus aethiops
Culicidae
Dengue
Dengue Virus
Vero Cells
Virus Replication
Dengue virus
Mammalia
dc.description.none.fl_txt_mv The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM.
Fil:Talarico, L.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Noseda, M.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Fil:Damonte, E.B. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description The antiviral activity against dengue virus-2 (DENV-2) of carrageenans reported here has shown a differential susceptibility of C6/36 HT and Vero cells, taken as models of mosquito and mammalian cells, depending on the structural class of polysaccharides: all polysaccharides blocked DENV-2 infection in monkey Vero cells, but only iota-carrageenans were virus inhibitors in mosquito cells. However, iota-carrageenans were less effective in mosquito cells in comparison with mammalian cells with effective concentration 50% (EC50) values in C6/36 HT cells 4.9-17.5-fold higher than in Vero cells, as determined by virus yield reduction assay. The mode of action of iota-carrageenan in both cell types was strikingly different: in Vero cells the inhibitory activity was exerted only at the initiation of the cycle, affecting virion binding, whereas in mosquito cells DENV-2 adsorption was not affected and comparable levels of inhibition were obtained if the compound was added to cells together with the virus, after 8 h of infection or by cell pretreatment before infection. Furthermore, iota-carrageenans induced a subtle alteration in mosquito cells, detected by cell proliferation and protein synthesis analyses, suggesting that a probable cellular target may be responsible for the refractory state of mosquito cells to DENV-2 infection produced by this class of polysulfates. The failure of iota-carrageenan to block DENV-2 adsorption to mosquito cells appeared to be related to the low presence of adequate heparin sulfate (HS) in C6/36 HT cell surface and is indicative of a differential participation of HS residues for DENV-2 entry in both types of cells. © 2011 SGM.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_Talarico
url http://hdl.handle.net/20.500.12110/paper_00221317_v92_n6_p1332_Talarico
dc.language.none.fl_str_mv eng
language eng
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rights_invalid_str_mv http://creativecommons.org/licenses/by/2.5/ar
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dc.source.none.fl_str_mv J. Gen. Virol. 2011;92(6):1332-1342
reponame:Biblioteca Digital (UBA-FCEN)
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institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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