First study of different insect cells to triatoma virus infection

Autores
Susevich, Maria Laura; Marti, Gerardo Anibal; Metz Germán Ernesto; Echeverria, Maria Gabriela
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas disease, which is endemic in Latin America but also expanding its worldwide distribution. To this end, we attempted virus replication in Diptera, Aedes albopictus (clone C6/36) and Lepidoptera (Spodoptera frugiperda (SF9, SF21) and HighFive (H5) cell lines. The methodologies used were transfection process, direct inoculation (purified virus), and inoculation of purified virus with trypsin. Results were confirmed by SDS-PAGE, Western blotting, RT-PCR, electron microscopic and immunofluorescence. According to the results obtained, further analysis of susceptibility / infection of H5 cells to TrV required to be studied.
Fil: Susevich, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Metz Germán Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Echeverria, Maria Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Dicistrovirus
Rt-Pcr
Insect Cells
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/11127
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First study of different insect cells to triatoma virus infectionSusevich, Maria LauraMarti, Gerardo AnibalMetz Germán ErnestoEcheverria, Maria GabrielaDicistrovirusRt-PcrInsect Cellshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas disease, which is endemic in Latin America but also expanding its worldwide distribution. To this end, we attempted virus replication in Diptera, Aedes albopictus (clone C6/36) and Lepidoptera (Spodoptera frugiperda (SF9, SF21) and HighFive (H5) cell lines. The methodologies used were transfection process, direct inoculation (purified virus), and inoculation of purified virus with trypsin. Results were confirmed by SDS-PAGE, Western blotting, RT-PCR, electron microscopic and immunofluorescence. According to the results obtained, further analysis of susceptibility / infection of H5 cells to TrV required to be studied.Fil: Susevich, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; ArgentinaFil: Metz Germán Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Echeverria, Maria Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2015-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/11127Susevich, Maria Laura; Marti, Gerardo Anibal; Metz Germán Ernesto; Echeverria, Maria Gabriela; First study of different insect cells to triatoma virus infection; Springer; Current Microbiology; 70; 4; 3-2015; 470-4750343-8651enginfo:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00284-014-0746-xinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-014-0746-xinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:42:02Zoai:ri.conicet.gov.ar:11336/11127instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:42:03.198CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First study of different insect cells to triatoma virus infection
title First study of different insect cells to triatoma virus infection
spellingShingle First study of different insect cells to triatoma virus infection
Susevich, Maria Laura
Dicistrovirus
Rt-Pcr
Insect Cells
title_short First study of different insect cells to triatoma virus infection
title_full First study of different insect cells to triatoma virus infection
title_fullStr First study of different insect cells to triatoma virus infection
title_full_unstemmed First study of different insect cells to triatoma virus infection
title_sort First study of different insect cells to triatoma virus infection
dc.creator.none.fl_str_mv Susevich, Maria Laura
Marti, Gerardo Anibal
Metz Germán Ernesto
Echeverria, Maria Gabriela
author Susevich, Maria Laura
author_facet Susevich, Maria Laura
Marti, Gerardo Anibal
Metz Germán Ernesto
Echeverria, Maria Gabriela
author_role author
author2 Marti, Gerardo Anibal
Metz Germán Ernesto
Echeverria, Maria Gabriela
author2_role author
author
author
dc.subject.none.fl_str_mv Dicistrovirus
Rt-Pcr
Insect Cells
topic Dicistrovirus
Rt-Pcr
Insect Cells
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas disease, which is endemic in Latin America but also expanding its worldwide distribution. To this end, we attempted virus replication in Diptera, Aedes albopictus (clone C6/36) and Lepidoptera (Spodoptera frugiperda (SF9, SF21) and HighFive (H5) cell lines. The methodologies used were transfection process, direct inoculation (purified virus), and inoculation of purified virus with trypsin. Results were confirmed by SDS-PAGE, Western blotting, RT-PCR, electron microscopic and immunofluorescence. According to the results obtained, further analysis of susceptibility / infection of H5 cells to TrV required to be studied.
Fil: Susevich, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Marti, Gerardo Anibal. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico la Plata. Centro de Estudios Parasitológicos y de Vectores (i); Argentina. Universidad Nacional de La Plata; Argentina
Fil: Metz Germán Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Echeverria, Maria Gabriela. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The use of viruses for biological control is a new option to be considered. The family Dicistroviridae, which affects only invertebrates, is one of the families that have been proposed for this purpose. The Triatoma virus (TrV), a member of this family, affects triatomine transmitters of Chagas disease, which is endemic in Latin America but also expanding its worldwide distribution. To this end, we attempted virus replication in Diptera, Aedes albopictus (clone C6/36) and Lepidoptera (Spodoptera frugiperda (SF9, SF21) and HighFive (H5) cell lines. The methodologies used were transfection process, direct inoculation (purified virus), and inoculation of purified virus with trypsin. Results were confirmed by SDS-PAGE, Western blotting, RT-PCR, electron microscopic and immunofluorescence. According to the results obtained, further analysis of susceptibility / infection of H5 cells to TrV required to be studied.
publishDate 2015
dc.date.none.fl_str_mv 2015-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/11127
Susevich, Maria Laura; Marti, Gerardo Anibal; Metz Germán Ernesto; Echeverria, Maria Gabriela; First study of different insect cells to triatoma virus infection; Springer; Current Microbiology; 70; 4; 3-2015; 470-475
0343-8651
url http://hdl.handle.net/11336/11127
identifier_str_mv Susevich, Maria Laura; Marti, Gerardo Anibal; Metz Germán Ernesto; Echeverria, Maria Gabriela; First study of different insect cells to triatoma virus infection; Springer; Current Microbiology; 70; 4; 3-2015; 470-475
0343-8651
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://link.springer.com/article/10.1007/s00284-014-0746-x
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-014-0746-x
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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