Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina

Autores
Ferrufino, Cecilia Gabriela Paola; Figini, Emilio Eduardo; Gonzalez, Fernanda; Dus Santos, María José; Salvador, Ricardo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bee viruses cause significant losses in honey production around the world. Previous studies detected at least 8 different viruses in Argentinean honey producer apiaries. Queen rearing activity is used by beekeepers to increase brood and honey production, to start new colonies and to change the colony genetic background. The aim of this study was to detect the presence of bee viruses in an Argentinean queen rearing apiary with highly increased queen pupae mortality. Samples of pupae and royal jelly were collected from queen rearing hives and analyzed by RT-PCR. Sampled pupae with viral symptoms were found to be positive for acute bee paralysis virus (ABPV) and black queen cell virus (BQCV). Gene fragments from ABPV and BQCV positive samples were amplified and sequenced in order to perform phylogenetic analysis. To our knowledge, this is the first report of bee virus detection in a queen rearing apiary from Argentina and the first phylogenetic analysis using local nucleotide sequences data.
Fil: Ferrufino, Cecilia Gabriela Paola. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
Fil: Figini, Emilio Eduardo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Gonzalez, Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
Materia
ARGENTINEAN QUEEN REARING APIARY
HONEYBEE
ACUTE BEE PARALYSIS VIRUS
BLACK QUEEN CELL VIRUS
PHYLOGENETIC ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/145975
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/145975
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection of Honeybee Viruses in a Queen-Rearing Apiary from ArgentinaFerrufino, Cecilia Gabriela PaolaFigini, Emilio EduardoGonzalez, FernandaDus Santos, María JoséSalvador, RicardoARGENTINEAN QUEEN REARING APIARYHONEYBEEACUTE BEE PARALYSIS VIRUSBLACK QUEEN CELL VIRUSPHYLOGENETIC ANALYSIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Bee viruses cause significant losses in honey production around the world. Previous studies detected at least 8 different viruses in Argentinean honey producer apiaries. Queen rearing activity is used by beekeepers to increase brood and honey production, to start new colonies and to change the colony genetic background. The aim of this study was to detect the presence of bee viruses in an Argentinean queen rearing apiary with highly increased queen pupae mortality. Samples of pupae and royal jelly were collected from queen rearing hives and analyzed by RT-PCR. Sampled pupae with viral symptoms were found to be positive for acute bee paralysis virus (ABPV) and black queen cell virus (BQCV). Gene fragments from ABPV and BQCV positive samples were amplified and sequenced in order to perform phylogenetic analysis. To our knowledge, this is the first report of bee virus detection in a queen rearing apiary from Argentina and the first phylogenetic analysis using local nucleotide sequences data.Fil: Ferrufino, Cecilia Gabriela Paola. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; ArgentinaFil: Figini, Emilio Eduardo. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Gonzalez, Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; ArgentinaFil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; ArgentinaCrimson Publishers2020-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/145975Ferrufino, Cecilia Gabriela Paola; Figini, Emilio Eduardo; Gonzalez, Fernanda; Dus Santos, María José; Salvador, Ricardo; Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina; Crimson Publishers; Modern Concepts & Developments in Agronomy; 6; 1; 3-2020; 1-42637-7659CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://crimsonpublishers.com/mcda/fulltext/MCDA.000630.phpinfo:eu-repo/semantics/altIdentifier/doi/10.31031/MCDA.2020.06.000630info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:50Zoai:ri.conicet.gov.ar:11336/145975instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:50.635CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina
title Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina
spellingShingle Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina
Ferrufino, Cecilia Gabriela Paola
ARGENTINEAN QUEEN REARING APIARY
HONEYBEE
ACUTE BEE PARALYSIS VIRUS
BLACK QUEEN CELL VIRUS
PHYLOGENETIC ANALYSIS
title_short Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina
title_full Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina
title_fullStr Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina
title_full_unstemmed Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina
title_sort Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina
dc.creator.none.fl_str_mv Ferrufino, Cecilia Gabriela Paola
Figini, Emilio Eduardo
Gonzalez, Fernanda
Dus Santos, María José
Salvador, Ricardo
author Ferrufino, Cecilia Gabriela Paola
author_facet Ferrufino, Cecilia Gabriela Paola
Figini, Emilio Eduardo
Gonzalez, Fernanda
Dus Santos, María José
Salvador, Ricardo
author_role author
author2 Figini, Emilio Eduardo
Gonzalez, Fernanda
Dus Santos, María José
Salvador, Ricardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv ARGENTINEAN QUEEN REARING APIARY
HONEYBEE
ACUTE BEE PARALYSIS VIRUS
BLACK QUEEN CELL VIRUS
PHYLOGENETIC ANALYSIS
topic ARGENTINEAN QUEEN REARING APIARY
HONEYBEE
ACUTE BEE PARALYSIS VIRUS
BLACK QUEEN CELL VIRUS
PHYLOGENETIC ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Bee viruses cause significant losses in honey production around the world. Previous studies detected at least 8 different viruses in Argentinean honey producer apiaries. Queen rearing activity is used by beekeepers to increase brood and honey production, to start new colonies and to change the colony genetic background. The aim of this study was to detect the presence of bee viruses in an Argentinean queen rearing apiary with highly increased queen pupae mortality. Samples of pupae and royal jelly were collected from queen rearing hives and analyzed by RT-PCR. Sampled pupae with viral symptoms were found to be positive for acute bee paralysis virus (ABPV) and black queen cell virus (BQCV). Gene fragments from ABPV and BQCV positive samples were amplified and sequenced in order to perform phylogenetic analysis. To our knowledge, this is the first report of bee virus detection in a queen rearing apiary from Argentina and the first phylogenetic analysis using local nucleotide sequences data.
Fil: Ferrufino, Cecilia Gabriela Paola. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
Fil: Figini, Emilio Eduardo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Gonzalez, Fernanda. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Dus Santos, María José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
Fil: Salvador, Ricardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Microbiología y Zoología Agrícola; Argentina
description Bee viruses cause significant losses in honey production around the world. Previous studies detected at least 8 different viruses in Argentinean honey producer apiaries. Queen rearing activity is used by beekeepers to increase brood and honey production, to start new colonies and to change the colony genetic background. The aim of this study was to detect the presence of bee viruses in an Argentinean queen rearing apiary with highly increased queen pupae mortality. Samples of pupae and royal jelly were collected from queen rearing hives and analyzed by RT-PCR. Sampled pupae with viral symptoms were found to be positive for acute bee paralysis virus (ABPV) and black queen cell virus (BQCV). Gene fragments from ABPV and BQCV positive samples were amplified and sequenced in order to perform phylogenetic analysis. To our knowledge, this is the first report of bee virus detection in a queen rearing apiary from Argentina and the first phylogenetic analysis using local nucleotide sequences data.
publishDate 2020
dc.date.none.fl_str_mv 2020-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/145975
Ferrufino, Cecilia Gabriela Paola; Figini, Emilio Eduardo; Gonzalez, Fernanda; Dus Santos, María José; Salvador, Ricardo; Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina; Crimson Publishers; Modern Concepts & Developments in Agronomy; 6; 1; 3-2020; 1-4
2637-7659
CONICET Digital
CONICET
url http://hdl.handle.net/11336/145975
identifier_str_mv Ferrufino, Cecilia Gabriela Paola; Figini, Emilio Eduardo; Gonzalez, Fernanda; Dus Santos, María José; Salvador, Ricardo; Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina; Crimson Publishers; Modern Concepts & Developments in Agronomy; 6; 1; 3-2020; 1-4
2637-7659
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://crimsonpublishers.com/mcda/fulltext/MCDA.000630.php
info:eu-repo/semantics/altIdentifier/doi/10.31031/MCDA.2020.06.000630
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Crimson Publishers
publisher.none.fl_str_mv Crimson Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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