Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii
- Autores
- Carral, Liliana; Kaufer, Federico; Pardini, Lais; Durlach, Ricardo; Moré, Gastón Andrés; Venturini, María Cecilia; Freuler, Cristina
- Año de publicación
- 2018
- Idioma
- español castellano
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Background. Congenital toxoplasmosis diagnosis in the newborn is a very important issue due to the need for early treatment to prevent future sequels. Aim: To compare available methods at the institution for the diagnosis of congenital toxoplasmosis. Material and Methods: In this study we have evaluated the different diagnostic tests used in 67 congenital exposed newborns, including serological tests, PCR, parasite isolation and molecular characterization. Results: The ISAGA IgM and IgA tests showed sensitivity (Se) of 87 and 91%, respectively, and specificity (Sp) of 100%. When ISAGA IgM and IgA were performed simultaneously, the Se increased to 98% and the Sp was 100%. The presence of IgE contributed to the diagnosis when it was detected in the child’s serum but not in maternal blood. In four congenital infected children the parasite was isolated and genotyped: one was genotype II and the other three were “atypical” genotypes. No parasite was isolated in children without congenital toxoplasmosis. Discussion: Overall, serological tests showed a good diagnostic performance although in one case they were all negative and isolation was the only tool to identify the infection. We conclude that it is essential to use all diagnostic tests in every single exposed child, including if possible, molecular characterization due to its epidemiological implication.
Facultad de Ciencias Veterinarias - Materia
-
Ciencias Veterinarias
Congenital toxoplasmosis
Serology
Parasitic isolation
PCR
Molecular characterization
Toxoplasmosis Congénita
Serología
Aislamiento del parásito
RPC
Caracterización molecular - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc/4.0/
- Repositorio
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/109199
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Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondiiCongenital toxoplasmosis: Serology, PCR, parasite isolation and molecular characterization of Toxoplasma gondiiCarral, LilianaKaufer, FedericoPardini, LaisDurlach, RicardoMoré, Gastón AndrésVenturini, María CeciliaFreuler, CristinaCiencias VeterinariasCongenital toxoplasmosisSerologyParasitic isolationPCRMolecular characterizationToxoplasmosis CongénitaSerologíaAislamiento del parásitoRPCCaracterización molecularBackground. Congenital toxoplasmosis diagnosis in the newborn is a very important issue due to the need for early treatment to prevent future sequels. Aim: To compare available methods at the institution for the diagnosis of congenital toxoplasmosis. Material and Methods: In this study we have evaluated the different diagnostic tests used in 67 congenital exposed newborns, including serological tests, PCR, parasite isolation and molecular characterization. Results: The ISAGA IgM and IgA tests showed sensitivity (Se) of 87 and 91%, respectively, and specificity (Sp) of 100%. When ISAGA IgM and IgA were performed simultaneously, the Se increased to 98% and the Sp was 100%. The presence of IgE contributed to the diagnosis when it was detected in the child’s serum but not in maternal blood. In four congenital infected children the parasite was isolated and genotyped: one was genotype II and the other three were “atypical” genotypes. No parasite was isolated in children without congenital toxoplasmosis. Discussion: Overall, serological tests showed a good diagnostic performance although in one case they were all negative and isolation was the only tool to identify the infection. We conclude that it is essential to use all diagnostic tests in every single exposed child, including if possible, molecular characterization due to its epidemiological implication.Facultad de Ciencias Veterinarias2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf36-40http://sedici.unlp.edu.ar/handle/10915/109199spainfo:eu-repo/semantics/altIdentifier/issn/0716-1018info:eu-repo/semantics/altIdentifier/doi/10.4067/s0716-10182018000100036info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:56:57Zoai:sedici.unlp.edu.ar:10915/109199Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:56:57.952SEDICI (UNLP) - Universidad Nacional de La Platafalse |
dc.title.none.fl_str_mv |
Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii Congenital toxoplasmosis: Serology, PCR, parasite isolation and molecular characterization of Toxoplasma gondii |
title |
Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii |
spellingShingle |
Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii Carral, Liliana Ciencias Veterinarias Congenital toxoplasmosis Serology Parasitic isolation PCR Molecular characterization Toxoplasmosis Congénita Serología Aislamiento del parásito RPC Caracterización molecular |
title_short |
Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii |
title_full |
Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii |
title_fullStr |
Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii |
title_full_unstemmed |
Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii |
title_sort |
Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii |
dc.creator.none.fl_str_mv |
Carral, Liliana Kaufer, Federico Pardini, Lais Durlach, Ricardo Moré, Gastón Andrés Venturini, María Cecilia Freuler, Cristina |
author |
Carral, Liliana |
author_facet |
Carral, Liliana Kaufer, Federico Pardini, Lais Durlach, Ricardo Moré, Gastón Andrés Venturini, María Cecilia Freuler, Cristina |
author_role |
author |
author2 |
Kaufer, Federico Pardini, Lais Durlach, Ricardo Moré, Gastón Andrés Venturini, María Cecilia Freuler, Cristina |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Ciencias Veterinarias Congenital toxoplasmosis Serology Parasitic isolation PCR Molecular characterization Toxoplasmosis Congénita Serología Aislamiento del parásito RPC Caracterización molecular |
topic |
Ciencias Veterinarias Congenital toxoplasmosis Serology Parasitic isolation PCR Molecular characterization Toxoplasmosis Congénita Serología Aislamiento del parásito RPC Caracterización molecular |
dc.description.none.fl_txt_mv |
Background. Congenital toxoplasmosis diagnosis in the newborn is a very important issue due to the need for early treatment to prevent future sequels. Aim: To compare available methods at the institution for the diagnosis of congenital toxoplasmosis. Material and Methods: In this study we have evaluated the different diagnostic tests used in 67 congenital exposed newborns, including serological tests, PCR, parasite isolation and molecular characterization. Results: The ISAGA IgM and IgA tests showed sensitivity (Se) of 87 and 91%, respectively, and specificity (Sp) of 100%. When ISAGA IgM and IgA were performed simultaneously, the Se increased to 98% and the Sp was 100%. The presence of IgE contributed to the diagnosis when it was detected in the child’s serum but not in maternal blood. In four congenital infected children the parasite was isolated and genotyped: one was genotype II and the other three were “atypical” genotypes. No parasite was isolated in children without congenital toxoplasmosis. Discussion: Overall, serological tests showed a good diagnostic performance although in one case they were all negative and isolation was the only tool to identify the infection. We conclude that it is essential to use all diagnostic tests in every single exposed child, including if possible, molecular characterization due to its epidemiological implication. Facultad de Ciencias Veterinarias |
description |
Background. Congenital toxoplasmosis diagnosis in the newborn is a very important issue due to the need for early treatment to prevent future sequels. Aim: To compare available methods at the institution for the diagnosis of congenital toxoplasmosis. Material and Methods: In this study we have evaluated the different diagnostic tests used in 67 congenital exposed newborns, including serological tests, PCR, parasite isolation and molecular characterization. Results: The ISAGA IgM and IgA tests showed sensitivity (Se) of 87 and 91%, respectively, and specificity (Sp) of 100%. When ISAGA IgM and IgA were performed simultaneously, the Se increased to 98% and the Sp was 100%. The presence of IgE contributed to the diagnosis when it was detected in the child’s serum but not in maternal blood. In four congenital infected children the parasite was isolated and genotyped: one was genotype II and the other three were “atypical” genotypes. No parasite was isolated in children without congenital toxoplasmosis. Discussion: Overall, serological tests showed a good diagnostic performance although in one case they were all negative and isolation was the only tool to identify the infection. We conclude that it is essential to use all diagnostic tests in every single exposed child, including if possible, molecular characterization due to its epidemiological implication. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 |
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