Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii

Autores
Carral, Liliana; Kaufer, Federico; Pardini, Lais; Durlach, Ricardo; Moré, Gastón Andrés; Venturini, María Cecilia; Freuler, Cristina
Año de publicación
2018
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background. Congenital toxoplasmosis diagnosis in the newborn is a very important issue due to the need for early treatment to prevent future sequels. Aim: To compare available methods at the institution for the diagnosis of congenital toxoplasmosis. Material and Methods: In this study we have evaluated the different diagnostic tests used in 67 congenital exposed newborns, including serological tests, PCR, parasite isolation and molecular characterization. Results: The ISAGA IgM and IgA tests showed sensitivity (Se) of 87 and 91%, respectively, and specificity (Sp) of 100%. When ISAGA IgM and IgA were performed simultaneously, the Se increased to 98% and the Sp was 100%. The presence of IgE contributed to the diagnosis when it was detected in the child’s serum but not in maternal blood. In four congenital infected children the parasite was isolated and genotyped: one was genotype II and the other three were “atypical” genotypes. No parasite was isolated in children without congenital toxoplasmosis. Discussion: Overall, serological tests showed a good diagnostic performance although in one case they were all negative and isolation was the only tool to identify the infection. We conclude that it is essential to use all diagnostic tests in every single exposed child, including if possible, molecular characterization due to its epidemiological implication.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
Congenital toxoplasmosis
Serology
Parasitic isolation
PCR
Molecular characterization
Toxoplasmosis Congénita
Serología
Aislamiento del parásito
RPC
Caracterización molecular
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/109199

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oai_identifier_str oai:sedici.unlp.edu.ar:10915/109199
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondiiCongenital toxoplasmosis: Serology, PCR, parasite isolation and molecular characterization of Toxoplasma gondiiCarral, LilianaKaufer, FedericoPardini, LaisDurlach, RicardoMoré, Gastón AndrésVenturini, María CeciliaFreuler, CristinaCiencias VeterinariasCongenital toxoplasmosisSerologyParasitic isolationPCRMolecular characterizationToxoplasmosis CongénitaSerologíaAislamiento del parásitoRPCCaracterización molecularBackground. Congenital toxoplasmosis diagnosis in the newborn is a very important issue due to the need for early treatment to prevent future sequels. Aim: To compare available methods at the institution for the diagnosis of congenital toxoplasmosis. Material and Methods: In this study we have evaluated the different diagnostic tests used in 67 congenital exposed newborns, including serological tests, PCR, parasite isolation and molecular characterization. Results: The ISAGA IgM and IgA tests showed sensitivity (Se) of 87 and 91%, respectively, and specificity (Sp) of 100%. When ISAGA IgM and IgA were performed simultaneously, the Se increased to 98% and the Sp was 100%. The presence of IgE contributed to the diagnosis when it was detected in the child’s serum but not in maternal blood. In four congenital infected children the parasite was isolated and genotyped: one was genotype II and the other three were “atypical” genotypes. No parasite was isolated in children without congenital toxoplasmosis. Discussion: Overall, serological tests showed a good diagnostic performance although in one case they were all negative and isolation was the only tool to identify the infection. We conclude that it is essential to use all diagnostic tests in every single exposed child, including if possible, molecular characterization due to its epidemiological implication.Facultad de Ciencias Veterinarias2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf36-40http://sedici.unlp.edu.ar/handle/10915/109199spainfo:eu-repo/semantics/altIdentifier/issn/0716-1018info:eu-repo/semantics/altIdentifier/doi/10.4067/s0716-10182018000100036info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:56:57Zoai:sedici.unlp.edu.ar:10915/109199Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:56:57.952SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii
Congenital toxoplasmosis: Serology, PCR, parasite isolation and molecular characterization of Toxoplasma gondii
title Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii
spellingShingle Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii
Carral, Liliana
Ciencias Veterinarias
Congenital toxoplasmosis
Serology
Parasitic isolation
PCR
Molecular characterization
Toxoplasmosis Congénita
Serología
Aislamiento del parásito
RPC
Caracterización molecular
title_short Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii
title_full Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii
title_fullStr Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii
title_full_unstemmed Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii
title_sort Toxoplasmosis congénita: diagnóstico serológico, RPC, aislamiento y caracterización molecular de Toxoplasma gondii
dc.creator.none.fl_str_mv Carral, Liliana
Kaufer, Federico
Pardini, Lais
Durlach, Ricardo
Moré, Gastón Andrés
Venturini, María Cecilia
Freuler, Cristina
author Carral, Liliana
author_facet Carral, Liliana
Kaufer, Federico
Pardini, Lais
Durlach, Ricardo
Moré, Gastón Andrés
Venturini, María Cecilia
Freuler, Cristina
author_role author
author2 Kaufer, Federico
Pardini, Lais
Durlach, Ricardo
Moré, Gastón Andrés
Venturini, María Cecilia
Freuler, Cristina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
Congenital toxoplasmosis
Serology
Parasitic isolation
PCR
Molecular characterization
Toxoplasmosis Congénita
Serología
Aislamiento del parásito
RPC
Caracterización molecular
topic Ciencias Veterinarias
Congenital toxoplasmosis
Serology
Parasitic isolation
PCR
Molecular characterization
Toxoplasmosis Congénita
Serología
Aislamiento del parásito
RPC
Caracterización molecular
dc.description.none.fl_txt_mv Background. Congenital toxoplasmosis diagnosis in the newborn is a very important issue due to the need for early treatment to prevent future sequels. Aim: To compare available methods at the institution for the diagnosis of congenital toxoplasmosis. Material and Methods: In this study we have evaluated the different diagnostic tests used in 67 congenital exposed newborns, including serological tests, PCR, parasite isolation and molecular characterization. Results: The ISAGA IgM and IgA tests showed sensitivity (Se) of 87 and 91%, respectively, and specificity (Sp) of 100%. When ISAGA IgM and IgA were performed simultaneously, the Se increased to 98% and the Sp was 100%. The presence of IgE contributed to the diagnosis when it was detected in the child’s serum but not in maternal blood. In four congenital infected children the parasite was isolated and genotyped: one was genotype II and the other three were “atypical” genotypes. No parasite was isolated in children without congenital toxoplasmosis. Discussion: Overall, serological tests showed a good diagnostic performance although in one case they were all negative and isolation was the only tool to identify the infection. We conclude that it is essential to use all diagnostic tests in every single exposed child, including if possible, molecular characterization due to its epidemiological implication.
Facultad de Ciencias Veterinarias
description Background. Congenital toxoplasmosis diagnosis in the newborn is a very important issue due to the need for early treatment to prevent future sequels. Aim: To compare available methods at the institution for the diagnosis of congenital toxoplasmosis. Material and Methods: In this study we have evaluated the different diagnostic tests used in 67 congenital exposed newborns, including serological tests, PCR, parasite isolation and molecular characterization. Results: The ISAGA IgM and IgA tests showed sensitivity (Se) of 87 and 91%, respectively, and specificity (Sp) of 100%. When ISAGA IgM and IgA were performed simultaneously, the Se increased to 98% and the Sp was 100%. The presence of IgE contributed to the diagnosis when it was detected in the child’s serum but not in maternal blood. In four congenital infected children the parasite was isolated and genotyped: one was genotype II and the other three were “atypical” genotypes. No parasite was isolated in children without congenital toxoplasmosis. Discussion: Overall, serological tests showed a good diagnostic performance although in one case they were all negative and isolation was the only tool to identify the infection. We conclude that it is essential to use all diagnostic tests in every single exposed child, including if possible, molecular characterization due to its epidemiological implication.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/109199
url http://sedici.unlp.edu.ar/handle/10915/109199
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0716-1018
info:eu-repo/semantics/altIdentifier/doi/10.4067/s0716-10182018000100036
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.format.none.fl_str_mv application/pdf
36-40
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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