Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis
- Autores
- Altcheh, Jaime; Diaz, N; Pepe, Carolina M; Martín, Valentina; Nigro, Monica; Freilij, Héctor; Angel, Sergio O.
- Año de publicación
- 2006
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Altcheh, Jaime. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Diaz, Natalia S. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Pepe, Carolina M. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Nigro, Monica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Freilij, Héctor. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Angel, Sergio O. CONICET-UNSAM. Laboratorio de Parasitología Molecular, Chascomús; Argentina.
The reactivity values of Toxoplasma gondii ROP2, GRA4, and GRA7 recombinant antigens (rAgs) were analyzed by immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) in 23 congenitally infected (I) and 36 noninfected (NI) infants. The reactivity values observed from the serum samples of I versus NI infants for each recombinant protein were 91% versus 67% for rROP2 (P = .05), 86% versus 29% for rGRA4 (P < .001), and 56% versus 11% for rGRA7 (P = .003). The follow-up showed that serum samples from NI infants became negative for specific IgG at 5.8 months (95% confidence interval [CI], 4.9-6.7) using a commercial assay; meanwhile, by specific recombinant protein ELISA, the samples became negative at 3.7 months with rROP2 (95% CI, 2.8-4.6), at 1.3 months with rGRA4 (95% CI, 0.8-1.8), and at 0.9 months with rGRA7 (95% CI, 0.5-1.3). Kinetic analysis also showed that serum samples from group I presented different IgG-profiles among rAgs. The rROP2 IgG profile was similar to that of the commercial assay, whereas rGRA4 and rGRA7 profiles showed a gradual decrease along the period of the study. The potential of the utility of rAgs to develop a diagnostic system that discriminates congenitally I infants from NI is discussed. - Fuente
- Diagnostic Microbiology and Infectious Disease 2006;56(2):161-165
- Materia
-
Toxoplasma
Toxoplasmosis Congénita
Etanercept
Diagnóstico - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- none
- Repositorio
- Institución
- Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
- OAI Identificador
- oai:sgc.anlis.gob.ar:123456789/2121
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Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosisAltcheh, JaimeDiaz, NPepe, Carolina MMartín, ValentinaNigro, MonicaFreilij, HéctorAngel, Sergio O.ToxoplasmaToxoplasmosis CongénitaEtanerceptDiagnósticoFil: Altcheh, Jaime. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.Fil: Diaz, Natalia S. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.Fil: Pepe, Carolina M. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.Fil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Nigro, Monica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Freilij, Héctor. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.Fil: Angel, Sergio O. CONICET-UNSAM. Laboratorio de Parasitología Molecular, Chascomús; Argentina.The reactivity values of Toxoplasma gondii ROP2, GRA4, and GRA7 recombinant antigens (rAgs) were analyzed by immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) in 23 congenitally infected (I) and 36 noninfected (NI) infants. The reactivity values observed from the serum samples of I versus NI infants for each recombinant protein were 91% versus 67% for rROP2 (P = .05), 86% versus 29% for rGRA4 (P < .001), and 56% versus 11% for rGRA7 (P = .003). The follow-up showed that serum samples from NI infants became negative for specific IgG at 5.8 months (95% confidence interval [CI], 4.9-6.7) using a commercial assay; meanwhile, by specific recombinant protein ELISA, the samples became negative at 3.7 months with rROP2 (95% CI, 2.8-4.6), at 1.3 months with rGRA4 (95% CI, 0.8-1.8), and at 0.9 months with rGRA7 (95% CI, 0.5-1.3). Kinetic analysis also showed that serum samples from group I presented different IgG-profiles among rAgs. The rROP2 IgG profile was similar to that of the commercial assay, whereas rGRA4 and rGRA7 profiles showed a gradual decrease along the period of the study. The potential of the utility of rAgs to develop a diagnostic system that discriminates congenitally I infants from NI is discussed.2006-10info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0732-8893http://sgc.anlis.gob.ar/handle/123456789/212110.1016/j.diagmicrobio.2006.03.012Diagnostic Microbiology and Infectious Disease 2006;56(2):161-165reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISDiagnostic microbiology and infectious diseasenoneinfo:eu-repo/semantics/openAccesseng2025-09-04T11:18:15Zoai:sgc.anlis.gob.ar:123456789/2121Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:18:15.318Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false |
dc.title.none.fl_str_mv |
Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis |
title |
Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis |
spellingShingle |
Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis Altcheh, Jaime Toxoplasma Toxoplasmosis Congénita Etanercept Diagnóstico |
title_short |
Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis |
title_full |
Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis |
title_fullStr |
Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis |
title_full_unstemmed |
Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis |
title_sort |
Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis |
dc.creator.none.fl_str_mv |
Altcheh, Jaime Diaz, N Pepe, Carolina M Martín, Valentina Nigro, Monica Freilij, Héctor Angel, Sergio O. |
author |
Altcheh, Jaime |
author_facet |
Altcheh, Jaime Diaz, N Pepe, Carolina M Martín, Valentina Nigro, Monica Freilij, Héctor Angel, Sergio O. |
author_role |
author |
author2 |
Diaz, N Pepe, Carolina M Martín, Valentina Nigro, Monica Freilij, Héctor Angel, Sergio O. |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Toxoplasma Toxoplasmosis Congénita Etanercept Diagnóstico |
topic |
Toxoplasma Toxoplasmosis Congénita Etanercept Diagnóstico |
dc.description.none.fl_txt_mv |
Fil: Altcheh, Jaime. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina. Fil: Diaz, Natalia S. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina. Fil: Pepe, Carolina M. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina. Fil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Nigro, Monica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Freilij, Héctor. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina. Fil: Angel, Sergio O. CONICET-UNSAM. Laboratorio de Parasitología Molecular, Chascomús; Argentina. The reactivity values of Toxoplasma gondii ROP2, GRA4, and GRA7 recombinant antigens (rAgs) were analyzed by immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) in 23 congenitally infected (I) and 36 noninfected (NI) infants. The reactivity values observed from the serum samples of I versus NI infants for each recombinant protein were 91% versus 67% for rROP2 (P = .05), 86% versus 29% for rGRA4 (P < .001), and 56% versus 11% for rGRA7 (P = .003). The follow-up showed that serum samples from NI infants became negative for specific IgG at 5.8 months (95% confidence interval [CI], 4.9-6.7) using a commercial assay; meanwhile, by specific recombinant protein ELISA, the samples became negative at 3.7 months with rROP2 (95% CI, 2.8-4.6), at 1.3 months with rGRA4 (95% CI, 0.8-1.8), and at 0.9 months with rGRA7 (95% CI, 0.5-1.3). Kinetic analysis also showed that serum samples from group I presented different IgG-profiles among rAgs. The rROP2 IgG profile was similar to that of the commercial assay, whereas rGRA4 and rGRA7 profiles showed a gradual decrease along the period of the study. The potential of the utility of rAgs to develop a diagnostic system that discriminates congenitally I infants from NI is discussed. |
description |
Fil: Altcheh, Jaime. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10 |
dc.type.none.fl_str_mv |
info:ar-repo/semantics/articulo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
0732-8893 http://sgc.anlis.gob.ar/handle/123456789/2121 10.1016/j.diagmicrobio.2006.03.012 |
identifier_str_mv |
0732-8893 10.1016/j.diagmicrobio.2006.03.012 |
url |
http://sgc.anlis.gob.ar/handle/123456789/2121 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Diagnostic microbiology and infectious disease |
dc.rights.none.fl_str_mv |
none info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
none |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Diagnostic Microbiology and Infectious Disease 2006;56(2):161-165 reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
reponame_str |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
collection |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN |
instname_str |
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
instacron_str |
ANLIS |
institution |
ANLIS |
repository.name.fl_str_mv |
Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" |
repository.mail.fl_str_mv |
biblioteca@anlis.gov.ar |
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12.623145 |