Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis

Autores
Altcheh, Jaime; Diaz, N; Pepe, Carolina M; Martín, Valentina; Nigro, Monica; Freilij, Héctor; Angel, Sergio O.
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Altcheh, Jaime. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Diaz, Natalia S. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Pepe, Carolina M. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Nigro, Monica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Freilij, Héctor. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Angel, Sergio O. CONICET-UNSAM. Laboratorio de Parasitología Molecular, Chascomús; Argentina.
The reactivity values of Toxoplasma gondii ROP2, GRA4, and GRA7 recombinant antigens (rAgs) were analyzed by immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) in 23 congenitally infected (I) and 36 noninfected (NI) infants. The reactivity values observed from the serum samples of I versus NI infants for each recombinant protein were 91% versus 67% for rROP2 (P = .05), 86% versus 29% for rGRA4 (P < .001), and 56% versus 11% for rGRA7 (P = .003). The follow-up showed that serum samples from NI infants became negative for specific IgG at 5.8 months (95% confidence interval [CI], 4.9-6.7) using a commercial assay; meanwhile, by specific recombinant protein ELISA, the samples became negative at 3.7 months with rROP2 (95% CI, 2.8-4.6), at 1.3 months with rGRA4 (95% CI, 0.8-1.8), and at 0.9 months with rGRA7 (95% CI, 0.5-1.3). Kinetic analysis also showed that serum samples from group I presented different IgG-profiles among rAgs. The rROP2 IgG profile was similar to that of the commercial assay, whereas rGRA4 and rGRA7 profiles showed a gradual decrease along the period of the study. The potential of the utility of rAgs to develop a diagnostic system that discriminates congenitally I infants from NI is discussed.
Fuente
Diagnostic Microbiology and Infectious Disease 2006;56(2):161-165
Materia
Toxoplasma
Toxoplasmosis Congénita
Etanercept
Diagnóstico
Nivel de accesibilidad
acceso abierto
Condiciones de uso
none
Repositorio
Sistema de Gestión del Conocimiento ANLIS MALBRÁN
Institución
Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
OAI Identificador
oai:sgc.anlis.gob.ar:123456789/2121

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network_name_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
spelling Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosisAltcheh, JaimeDiaz, NPepe, Carolina MMartín, ValentinaNigro, MonicaFreilij, HéctorAngel, Sergio O.ToxoplasmaToxoplasmosis CongénitaEtanerceptDiagnósticoFil: Altcheh, Jaime. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.Fil: Diaz, Natalia S. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.Fil: Pepe, Carolina M. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.Fil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Nigro, Monica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.Fil: Freilij, Héctor. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.Fil: Angel, Sergio O. CONICET-UNSAM. Laboratorio de Parasitología Molecular, Chascomús; Argentina.The reactivity values of Toxoplasma gondii ROP2, GRA4, and GRA7 recombinant antigens (rAgs) were analyzed by immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) in 23 congenitally infected (I) and 36 noninfected (NI) infants. The reactivity values observed from the serum samples of I versus NI infants for each recombinant protein were 91% versus 67% for rROP2 (P = .05), 86% versus 29% for rGRA4 (P < .001), and 56% versus 11% for rGRA7 (P = .003). The follow-up showed that serum samples from NI infants became negative for specific IgG at 5.8 months (95% confidence interval [CI], 4.9-6.7) using a commercial assay; meanwhile, by specific recombinant protein ELISA, the samples became negative at 3.7 months with rROP2 (95% CI, 2.8-4.6), at 1.3 months with rGRA4 (95% CI, 0.8-1.8), and at 0.9 months with rGRA7 (95% CI, 0.5-1.3). Kinetic analysis also showed that serum samples from group I presented different IgG-profiles among rAgs. The rROP2 IgG profile was similar to that of the commercial assay, whereas rGRA4 and rGRA7 profiles showed a gradual decrease along the period of the study. The potential of the utility of rAgs to develop a diagnostic system that discriminates congenitally I infants from NI is discussed.2006-10info:ar-repo/semantics/articuloinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdf0732-8893http://sgc.anlis.gob.ar/handle/123456789/212110.1016/j.diagmicrobio.2006.03.012Diagnostic Microbiology and Infectious Disease 2006;56(2):161-165reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁNinstname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"instacron:ANLISDiagnostic microbiology and infectious diseasenoneinfo:eu-repo/semantics/openAccesseng2025-09-04T11:18:15Zoai:sgc.anlis.gob.ar:123456789/2121Institucionalhttp://sgc.anlis.gob.ar/Organismo científico-tecnológicoNo correspondehttp://sgc.anlis.gob.ar/oai/biblioteca@anlis.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:18:15.318Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"false
dc.title.none.fl_str_mv Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis
title Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis
spellingShingle Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis
Altcheh, Jaime
Toxoplasma
Toxoplasmosis Congénita
Etanercept
Diagnóstico
title_short Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis
title_full Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis
title_fullStr Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis
title_full_unstemmed Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis
title_sort Kinetic analysis of the humoral immune response against 3 Toxoplasma gondii-recombinant proteins in infants with suspected congenital toxoplasmosis
dc.creator.none.fl_str_mv Altcheh, Jaime
Diaz, N
Pepe, Carolina M
Martín, Valentina
Nigro, Monica
Freilij, Héctor
Angel, Sergio O.
author Altcheh, Jaime
author_facet Altcheh, Jaime
Diaz, N
Pepe, Carolina M
Martín, Valentina
Nigro, Monica
Freilij, Héctor
Angel, Sergio O.
author_role author
author2 Diaz, N
Pepe, Carolina M
Martín, Valentina
Nigro, Monica
Freilij, Héctor
Angel, Sergio O.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Toxoplasma
Toxoplasmosis Congénita
Etanercept
Diagnóstico
topic Toxoplasma
Toxoplasmosis Congénita
Etanercept
Diagnóstico
dc.description.none.fl_txt_mv Fil: Altcheh, Jaime. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Diaz, Natalia S. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Pepe, Carolina M. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Nigro, Monica. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina.
Fil: Freilij, Héctor. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
Fil: Angel, Sergio O. CONICET-UNSAM. Laboratorio de Parasitología Molecular, Chascomús; Argentina.
The reactivity values of Toxoplasma gondii ROP2, GRA4, and GRA7 recombinant antigens (rAgs) were analyzed by immunoglobulin G (IgG) enzyme-linked immunosorbent assay (ELISA) in 23 congenitally infected (I) and 36 noninfected (NI) infants. The reactivity values observed from the serum samples of I versus NI infants for each recombinant protein were 91% versus 67% for rROP2 (P = .05), 86% versus 29% for rGRA4 (P < .001), and 56% versus 11% for rGRA7 (P = .003). The follow-up showed that serum samples from NI infants became negative for specific IgG at 5.8 months (95% confidence interval [CI], 4.9-6.7) using a commercial assay; meanwhile, by specific recombinant protein ELISA, the samples became negative at 3.7 months with rROP2 (95% CI, 2.8-4.6), at 1.3 months with rGRA4 (95% CI, 0.8-1.8), and at 0.9 months with rGRA7 (95% CI, 0.5-1.3). Kinetic analysis also showed that serum samples from group I presented different IgG-profiles among rAgs. The rROP2 IgG profile was similar to that of the commercial assay, whereas rGRA4 and rGRA7 profiles showed a gradual decrease along the period of the study. The potential of the utility of rAgs to develop a diagnostic system that discriminates congenitally I infants from NI is discussed.
description Fil: Altcheh, Jaime. Hospital de Niños Dr. Ricardo Gutiérrez. Parasitología; Argentina.
publishDate 2006
dc.date.none.fl_str_mv 2006-10
dc.type.none.fl_str_mv info:ar-repo/semantics/articulo
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv 0732-8893
http://sgc.anlis.gob.ar/handle/123456789/2121
10.1016/j.diagmicrobio.2006.03.012
identifier_str_mv 0732-8893
10.1016/j.diagmicrobio.2006.03.012
url http://sgc.anlis.gob.ar/handle/123456789/2121
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Diagnostic microbiology and infectious disease
dc.rights.none.fl_str_mv none
info:eu-repo/semantics/openAccess
rights_invalid_str_mv none
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Diagnostic Microbiology and Infectious Disease 2006;56(2):161-165
reponame:Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname:Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron:ANLIS
reponame_str Sistema de Gestión del Conocimiento ANLIS MALBRÁN
collection Sistema de Gestión del Conocimiento ANLIS MALBRÁN
instname_str Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
instacron_str ANLIS
institution ANLIS
repository.name.fl_str_mv Sistema de Gestión del Conocimiento ANLIS MALBRÁN - Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán"
repository.mail.fl_str_mv biblioteca@anlis.gov.ar
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