Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina

Autores
Nosetto, Edgardo Omar; Echeverría, María Gabriela; Gimeno, Emilio Juan; Pecoraro, Marcelo Ricardo Ítalo; Etcheverrigaray, María Elisa
Año de publicación
1990
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Indirect and dot enzyme linked immunosorbent assays (ELISAs) were developed to detect antibodies to pseudorabies virus (PRV) in serum samples of 321 pigs. The Argentine CL 15 PRV strain was used as the source of virus. Nonidet P-40-solubilized infected and mock infected cell lysates were used as antigens. The results of these tests were compared with those of a blocking ELISA and virus neutralization (VN) test. There was a 100% agreement between the indirect and dot-ELISAs. Using VN as standard, the blocking ELISA and the indirect ELISA showed 97.6% and 100% relative sensitivity and 96.9% and 99% relative specificity respectively. The sensitivity of the blocking ELISA was lower than that of the other tests.
Facultad de Ciencias Veterinarias
Materia
Ciencias Veterinarias
ELISA
Técnicas de laboratorio
Porcinos
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/151289
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/151289
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in ArgentinaNosetto, Edgardo OmarEcheverría, María GabrielaGimeno, Emilio JuanPecoraro, Marcelo Ricardo ÍtaloEtcheverrigaray, María ElisaCiencias VeterinariasELISATécnicas de laboratorioPorcinosIndirect and dot enzyme linked immunosorbent assays (ELISAs) were developed to detect antibodies to pseudorabies virus (PRV) in serum samples of 321 pigs. The Argentine CL 15 PRV strain was used as the source of virus. Nonidet P-40-solubilized infected and mock infected cell lysates were used as antigens. The results of these tests were compared with those of a blocking ELISA and virus neutralization (VN) test. There was a 100% agreement between the indirect and dot-ELISAs. Using VN as standard, the blocking ELISA and the indirect ELISA showed 97.6% and 100% relative sensitivity and 96.9% and 99% relative specificity respectively. The sensitivity of the blocking ELISA was lower than that of the other tests.Facultad de Ciencias Veterinarias1990info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionObjeto de conferenciahttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdf59-64http://sedici.unlp.edu.ar/handle/10915/151289enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T11:11:00Zoai:sedici.unlp.edu.ar:10915/151289Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 11:11:00.754SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
spellingShingle Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
Nosetto, Edgardo Omar
Ciencias Veterinarias
ELISA
Técnicas de laboratorio
Porcinos
title_short Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title_full Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title_fullStr Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title_full_unstemmed Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
title_sort Development of an indirect and a dot ELISA to detect antibodies to porcine pseudorabies virus in Argentina
dc.creator.none.fl_str_mv Nosetto, Edgardo Omar
Echeverría, María Gabriela
Gimeno, Emilio Juan
Pecoraro, Marcelo Ricardo Ítalo
Etcheverrigaray, María Elisa
author Nosetto, Edgardo Omar
author_facet Nosetto, Edgardo Omar
Echeverría, María Gabriela
Gimeno, Emilio Juan
Pecoraro, Marcelo Ricardo Ítalo
Etcheverrigaray, María Elisa
author_role author
author2 Echeverría, María Gabriela
Gimeno, Emilio Juan
Pecoraro, Marcelo Ricardo Ítalo
Etcheverrigaray, María Elisa
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Veterinarias
ELISA
Técnicas de laboratorio
Porcinos
topic Ciencias Veterinarias
ELISA
Técnicas de laboratorio
Porcinos
dc.description.none.fl_txt_mv Indirect and dot enzyme linked immunosorbent assays (ELISAs) were developed to detect antibodies to pseudorabies virus (PRV) in serum samples of 321 pigs. The Argentine CL 15 PRV strain was used as the source of virus. Nonidet P-40-solubilized infected and mock infected cell lysates were used as antigens. The results of these tests were compared with those of a blocking ELISA and virus neutralization (VN) test. There was a 100% agreement between the indirect and dot-ELISAs. Using VN as standard, the blocking ELISA and the indirect ELISA showed 97.6% and 100% relative sensitivity and 96.9% and 99% relative specificity respectively. The sensitivity of the blocking ELISA was lower than that of the other tests.
Facultad de Ciencias Veterinarias
description Indirect and dot enzyme linked immunosorbent assays (ELISAs) were developed to detect antibodies to pseudorabies virus (PRV) in serum samples of 321 pigs. The Argentine CL 15 PRV strain was used as the source of virus. Nonidet P-40-solubilized infected and mock infected cell lysates were used as antigens. The results of these tests were compared with those of a blocking ELISA and virus neutralization (VN) test. There was a 100% agreement between the indirect and dot-ELISAs. Using VN as standard, the blocking ELISA and the indirect ELISA showed 97.6% and 100% relative sensitivity and 96.9% and 99% relative specificity respectively. The sensitivity of the blocking ELISA was lower than that of the other tests.
publishDate 1990
dc.date.none.fl_str_mv 1990
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
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format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/151289
url http://sedici.unlp.edu.ar/handle/10915/151289
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
59-64
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instname_str Universidad Nacional de La Plata
instacron_str UNLP
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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