Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome

Autores
Stringa, Pablo Luis; Andrés Moreno, Ane M.; Lausada, Natalia Raquel; Pastor Oliver, Cristina; Abate Zárate, Juan Cruz; Vecchio Dezillio, Leandro Emmanuel; Rumbo, Martín; Navarro Zorraquino, Marta; Hernández Oliveros, Francisco; Gondolesi, Gabriel Eduardo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Small Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the “pitfalls” of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.
Facultad de Ciencias Médicas
Laboratorio y Programa de Trasplante de Organos, Tejidos y Células
Instituto de Estudios Inmunológicos y Fisiopatológicos
Materia
Medicina
Ciencias Exactas
Intestinal transplantation
Surgical compliations
Rats
Microsurgery
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/101282
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/101282
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcomeStringa, Pablo LuisAndrés Moreno, Ane M.Lausada, Natalia RaquelPastor Oliver, CristinaAbate Zárate, Juan CruzVecchio Dezillio, Leandro EmmanuelRumbo, MartínNavarro Zorraquino, MartaHernández Oliveros, FranciscoGondolesi, Gabriel EduardoMedicinaCiencias ExactasIntestinal transplantationSurgical compliationsRatsMicrosurgerySmall Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the “pitfalls” of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.Facultad de Ciencias MédicasLaboratorio y Programa de Trasplante de Organos, Tejidos y CélulasInstituto de Estudios Inmunológicos y Fisiopatológicos2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/101282enginfo:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/48434info:eu-repo/semantics/altIdentifier/url/http://www.oatext.com/Small-bowel-transplantation-in-rats,-A-multicenter-experience-summarizing-the-pitfalls-to-be-overcome.phpinfo:eu-repo/semantics/altIdentifier/issn/2399-5289info:eu-repo/semantics/altIdentifier/doi/10.15761/TiT.1000217info:eu-repo/semantics/altIdentifier/hdl/11336/48434info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:53:11Zoai:sedici.unlp.edu.ar:10915/101282Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:53:11.501SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
spellingShingle Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
Stringa, Pablo Luis
Medicina
Ciencias Exactas
Intestinal transplantation
Surgical compliations
Rats
Microsurgery
title_short Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title_full Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title_fullStr Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title_full_unstemmed Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title_sort Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
dc.creator.none.fl_str_mv Stringa, Pablo Luis
Andrés Moreno, Ane M.
Lausada, Natalia Raquel
Pastor Oliver, Cristina
Abate Zárate, Juan Cruz
Vecchio Dezillio, Leandro Emmanuel
Rumbo, Martín
Navarro Zorraquino, Marta
Hernández Oliveros, Francisco
Gondolesi, Gabriel Eduardo
author Stringa, Pablo Luis
author_facet Stringa, Pablo Luis
Andrés Moreno, Ane M.
Lausada, Natalia Raquel
Pastor Oliver, Cristina
Abate Zárate, Juan Cruz
Vecchio Dezillio, Leandro Emmanuel
Rumbo, Martín
Navarro Zorraquino, Marta
Hernández Oliveros, Francisco
Gondolesi, Gabriel Eduardo
author_role author
author2 Andrés Moreno, Ane M.
Lausada, Natalia Raquel
Pastor Oliver, Cristina
Abate Zárate, Juan Cruz
Vecchio Dezillio, Leandro Emmanuel
Rumbo, Martín
Navarro Zorraquino, Marta
Hernández Oliveros, Francisco
Gondolesi, Gabriel Eduardo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Medicina
Ciencias Exactas
Intestinal transplantation
Surgical compliations
Rats
Microsurgery
topic Medicina
Ciencias Exactas
Intestinal transplantation
Surgical compliations
Rats
Microsurgery
dc.description.none.fl_txt_mv Small Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the “pitfalls” of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.
Facultad de Ciencias Médicas
Laboratorio y Programa de Trasplante de Organos, Tejidos y Células
Instituto de Estudios Inmunológicos y Fisiopatológicos
description Small Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the “pitfalls” of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/101282
url http://sedici.unlp.edu.ar/handle/10915/101282
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ri.conicet.gov.ar/11336/48434
info:eu-repo/semantics/altIdentifier/url/http://www.oatext.com/Small-bowel-transplantation-in-rats,-A-multicenter-experience-summarizing-the-pitfalls-to-be-overcome.php
info:eu-repo/semantics/altIdentifier/issn/2399-5289
info:eu-repo/semantics/altIdentifier/doi/10.15761/TiT.1000217
info:eu-repo/semantics/altIdentifier/hdl/11336/48434
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
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