Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome

Autores
Stringa, Pablo Luis; Andrés Moreno, Ane M.; Lausada, Natalia Raquel; Pastor Oliver, Cristina; Abate, Juan C.; Vecchio, Leandro; Rumbo, Martín; Navarro Zorraquino, Marta; Hernández Oliveros, Francisco; Gondolesi, Gabriel Eduardo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Small Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the “pitfalls” of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Andrés Moreno, Ane M.. La Paz University Hospital; España
Fil: Lausada, Natalia Raquel. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Pastor Oliver, Cristina. Biomedical Research Center from Aragón; España
Fil: Abate, Juan C.. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Vecchio, Leandro. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Rumbo, Martín. Comisión Nacional de Investigación Científica y Tecnológica; Chile. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Navarro Zorraquino, Marta. Biomedical Research Center from Aragón; España
Fil: Hernández Oliveros, Francisco. La Paz University Hospital; España
Fil: Gondolesi, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Materia
Intestinal Transplantation
Surgical Compliations
Rats
Microsurgery
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/48434
Acceder
id CONICETDig_a2f26539a48661dd4467729ffe5a5722
oai_identifier_str oai:ri.conicet.gov.ar:11336/48434
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcomeStringa, Pablo LuisAndrés Moreno, Ane M.Lausada, Natalia RaquelPastor Oliver, CristinaAbate, Juan C.Vecchio, LeandroRumbo, MartínNavarro Zorraquino, MartaHernández Oliveros, FranciscoGondolesi, Gabriel EduardoIntestinal TransplantationSurgical CompliationsRatsMicrosurgeryhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Small Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the “pitfalls” of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaFil: Andrés Moreno, Ane M.. La Paz University Hospital; EspañaFil: Lausada, Natalia Raquel. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaFil: Pastor Oliver, Cristina. Biomedical Research Center from Aragón; EspañaFil: Abate, Juan C.. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaFil: Vecchio, Leandro. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaFil: Rumbo, Martín. Comisión Nacional de Investigación Científica y Tecnológica; Chile. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaFil: Navarro Zorraquino, Marta. Biomedical Research Center from Aragón; EspañaFil: Hernández Oliveros, Francisco. La Paz University Hospital; EspañaFil: Gondolesi, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaOpen Access Text2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/48434Stringa, Pablo Luis; Andrés Moreno, Ane M.; Lausada, Natalia Raquel; Pastor Oliver, Cristina; Abate, Juan C.; et al.; Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome; Open Access Text; Transplantation Open; 2; 1; 12-2016; 1-72399-5289CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.oatext.com/Small-bowel-transplantation-in-rats,-A-multicenter-experience-summarizing-the-pitfalls-to-be-overcome.phpinfo:eu-repo/semantics/altIdentifier/doi/10.15761/TiT.1000217info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:03:57Zoai:ri.conicet.gov.ar:11336/48434instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:03:57.255CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
spellingShingle Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
Stringa, Pablo Luis
Intestinal Transplantation
Surgical Compliations
Rats
Microsurgery
title_short Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title_full Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title_fullStr Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title_full_unstemmed Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
title_sort Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome
dc.creator.none.fl_str_mv Stringa, Pablo Luis
Andrés Moreno, Ane M.
Lausada, Natalia Raquel
Pastor Oliver, Cristina
Abate, Juan C.
Vecchio, Leandro
Rumbo, Martín
Navarro Zorraquino, Marta
Hernández Oliveros, Francisco
Gondolesi, Gabriel Eduardo
author Stringa, Pablo Luis
author_facet Stringa, Pablo Luis
Andrés Moreno, Ane M.
Lausada, Natalia Raquel
Pastor Oliver, Cristina
Abate, Juan C.
Vecchio, Leandro
Rumbo, Martín
Navarro Zorraquino, Marta
Hernández Oliveros, Francisco
Gondolesi, Gabriel Eduardo
author_role author
author2 Andrés Moreno, Ane M.
Lausada, Natalia Raquel
Pastor Oliver, Cristina
Abate, Juan C.
Vecchio, Leandro
Rumbo, Martín
Navarro Zorraquino, Marta
Hernández Oliveros, Francisco
Gondolesi, Gabriel Eduardo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Intestinal Transplantation
Surgical Compliations
Rats
Microsurgery
topic Intestinal Transplantation
Surgical Compliations
Rats
Microsurgery
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Small Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the “pitfalls” of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Andrés Moreno, Ane M.. La Paz University Hospital; España
Fil: Lausada, Natalia Raquel. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Pastor Oliver, Cristina. Biomedical Research Center from Aragón; España
Fil: Abate, Juan C.. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Vecchio, Leandro. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Rumbo, Martín. Comisión Nacional de Investigación Científica y Tecnológica; Chile. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Navarro Zorraquino, Marta. Biomedical Research Center from Aragón; España
Fil: Hernández Oliveros, Francisco. La Paz University Hospital; España
Fil: Gondolesi, Gabriel Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Medicina Traslacional, Trasplante y Bioingeniería. Fundación Favaloro. Instituto de Medicina Traslacional, Trasplante y Bioingeniería; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
description Small Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the “pitfalls” of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/48434
Stringa, Pablo Luis; Andrés Moreno, Ane M.; Lausada, Natalia Raquel; Pastor Oliver, Cristina; Abate, Juan C.; et al.; Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome; Open Access Text; Transplantation Open; 2; 1; 12-2016; 1-7
2399-5289
CONICET Digital
CONICET
url http://hdl.handle.net/11336/48434
identifier_str_mv Stringa, Pablo Luis; Andrés Moreno, Ane M.; Lausada, Natalia Raquel; Pastor Oliver, Cristina; Abate, Juan C.; et al.; Small bowel transplantation in rats, a multicenter experience summarizing the pitfalls to be overcome; Open Access Text; Transplantation Open; 2; 1; 12-2016; 1-7
2399-5289
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.oatext.com/Small-bowel-transplantation-in-rats,-A-multicenter-experience-summarizing-the-pitfalls-to-be-overcome.php
info:eu-repo/semantics/altIdentifier/doi/10.15761/TiT.1000217
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Open Access Text
publisher.none.fl_str_mv Open Access Text
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269828476305408
score 13.13397

Publicaciones similares