Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation

Autores
Andres, Ane Miren; Santamaria, Monica; Hernandez Oliveros, Francisco; Guerra, Laura; Lopez, Sergio; Stringa, Pablo Luis; Vallejo, Maria Teresa; Largo, Carlota; Encinas, Jose Luis; Garcia de las Heras, Maria Soledad; Lopez Santamaria, Manuel; Tovar, Juan Antonio
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Experimental small bowel transplantation (SBT) in rats has been proven to be a useful tool for the study of ischemia-reperfusion and immunological aspects related to solid organ transplantation. However, the model is not completely refined, specialized literature is scarce and complex technical details are typically omitted or confusing. Most studies related to acute rejection (AR) use the orthotopic standard, with small sample sizes due to its high mortality, whereas those studying chronic rejection (CR) use the heterotopic standard, which allows longer term survival but does not exactly reflect the human clinical scenario. Various animal strains have been used, and the type of rejection and the timing of its analysis differ among authors. The double purpose of this study was to develop an improved unusual AR model of SBT using the heterotopic technique, and to elaborate a guide useful to implement experimental models for studying AR. We analyzed the model's technical details and expected difficulties in overcoming the learning curve for such a complex microsurgical model, identifying the potential problem areas and providing a step-by-step protocol and reference guide for future surgeons interested in the topic. We also discuss the historic and more recent options in the literature.
Fil: Andres, Ane Miren. Hospital Universitario La Paz; España
Fil: Santamaria, Monica. Hospital Universitario La Paz; España
Fil: Hernandez Oliveros, Francisco. Hospital Universitario La Paz; España
Fil: Guerra, Laura. Hospital Universitario La Paz; España
Fil: Lopez, Sergio. Hospital Universitario La Paz; España
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro. Área de Investigación y Desarrollo; Argentina
Fil: Vallejo, Maria Teresa. Hospital Universitario La Paz; España
Fil: Largo, Carlota. Hospital Universitario La Paz; España
Fil: Encinas, Jose Luis. Hospital Universitario La Paz; España
Fil: Garcia de las Heras, Maria Soledad. Universidad Rey Juan Carlos; España
Fil: Lopez Santamaria, Manuel. Hospital Universitario La Paz; España
Fil: Tovar, Juan Antonio. Hospital Universitario La Paz; España
Materia
Intestinal Transplantation
Rejection
Rats
Immunosupression
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72902
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/72902
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantationAndres, Ane MirenSantamaria, MonicaHernandez Oliveros, FranciscoGuerra, LauraLopez, SergioStringa, Pablo LuisVallejo, Maria TeresaLargo, CarlotaEncinas, Jose LuisGarcia de las Heras, Maria SoledadLopez Santamaria, ManuelTovar, Juan AntonioIntestinal TransplantationRejectionRatsImmunosupressionhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Experimental small bowel transplantation (SBT) in rats has been proven to be a useful tool for the study of ischemia-reperfusion and immunological aspects related to solid organ transplantation. However, the model is not completely refined, specialized literature is scarce and complex technical details are typically omitted or confusing. Most studies related to acute rejection (AR) use the orthotopic standard, with small sample sizes due to its high mortality, whereas those studying chronic rejection (CR) use the heterotopic standard, which allows longer term survival but does not exactly reflect the human clinical scenario. Various animal strains have been used, and the type of rejection and the timing of its analysis differ among authors. The double purpose of this study was to develop an improved unusual AR model of SBT using the heterotopic technique, and to elaborate a guide useful to implement experimental models for studying AR. We analyzed the model's technical details and expected difficulties in overcoming the learning curve for such a complex microsurgical model, identifying the potential problem areas and providing a step-by-step protocol and reference guide for future surgeons interested in the topic. We also discuss the historic and more recent options in the literature.Fil: Andres, Ane Miren. Hospital Universitario La Paz; EspañaFil: Santamaria, Monica. Hospital Universitario La Paz; EspañaFil: Hernandez Oliveros, Francisco. Hospital Universitario La Paz; EspañaFil: Guerra, Laura. Hospital Universitario La Paz; EspañaFil: Lopez, Sergio. Hospital Universitario La Paz; EspañaFil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro. Área de Investigación y Desarrollo; ArgentinaFil: Vallejo, Maria Teresa. Hospital Universitario La Paz; EspañaFil: Largo, Carlota. Hospital Universitario La Paz; EspañaFil: Encinas, Jose Luis. Hospital Universitario La Paz; EspañaFil: Garcia de las Heras, Maria Soledad. Universidad Rey Juan Carlos; EspañaFil: Lopez Santamaria, Manuel. Hospital Universitario La Paz; EspañaFil: Tovar, Juan Antonio. Hospital Universitario La Paz; EspañaElsevier Science2016-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72902Andres, Ane Miren; Santamaria, Monica; Hernandez Oliveros, Francisco; Guerra, Laura; Lopez, Sergio; et al.; Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation; Elsevier Science; Transplant Immunology; 36; 5-2016; 32-410966-3274CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.trim.2016.04.003info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0966327416300156info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:50:08Zoai:ri.conicet.gov.ar:11336/72902instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:50:08.392CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation
title Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation
spellingShingle Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation
Andres, Ane Miren
Intestinal Transplantation
Rejection
Rats
Immunosupression
title_short Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation
title_full Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation
title_fullStr Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation
title_full_unstemmed Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation
title_sort Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation
dc.creator.none.fl_str_mv Andres, Ane Miren
Santamaria, Monica
Hernandez Oliveros, Francisco
Guerra, Laura
Lopez, Sergio
Stringa, Pablo Luis
Vallejo, Maria Teresa
Largo, Carlota
Encinas, Jose Luis
Garcia de las Heras, Maria Soledad
Lopez Santamaria, Manuel
Tovar, Juan Antonio
author Andres, Ane Miren
author_facet Andres, Ane Miren
Santamaria, Monica
Hernandez Oliveros, Francisco
Guerra, Laura
Lopez, Sergio
Stringa, Pablo Luis
Vallejo, Maria Teresa
Largo, Carlota
Encinas, Jose Luis
Garcia de las Heras, Maria Soledad
Lopez Santamaria, Manuel
Tovar, Juan Antonio
author_role author
author2 Santamaria, Monica
Hernandez Oliveros, Francisco
Guerra, Laura
Lopez, Sergio
Stringa, Pablo Luis
Vallejo, Maria Teresa
Largo, Carlota
Encinas, Jose Luis
Garcia de las Heras, Maria Soledad
Lopez Santamaria, Manuel
Tovar, Juan Antonio
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Intestinal Transplantation
Rejection
Rats
Immunosupression
topic Intestinal Transplantation
Rejection
Rats
Immunosupression
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Experimental small bowel transplantation (SBT) in rats has been proven to be a useful tool for the study of ischemia-reperfusion and immunological aspects related to solid organ transplantation. However, the model is not completely refined, specialized literature is scarce and complex technical details are typically omitted or confusing. Most studies related to acute rejection (AR) use the orthotopic standard, with small sample sizes due to its high mortality, whereas those studying chronic rejection (CR) use the heterotopic standard, which allows longer term survival but does not exactly reflect the human clinical scenario. Various animal strains have been used, and the type of rejection and the timing of its analysis differ among authors. The double purpose of this study was to develop an improved unusual AR model of SBT using the heterotopic technique, and to elaborate a guide useful to implement experimental models for studying AR. We analyzed the model's technical details and expected difficulties in overcoming the learning curve for such a complex microsurgical model, identifying the potential problem areas and providing a step-by-step protocol and reference guide for future surgeons interested in the topic. We also discuss the historic and more recent options in the literature.
Fil: Andres, Ane Miren. Hospital Universitario La Paz; España
Fil: Santamaria, Monica. Hospital Universitario La Paz; España
Fil: Hernandez Oliveros, Francisco. Hospital Universitario La Paz; España
Fil: Guerra, Laura. Hospital Universitario La Paz; España
Fil: Lopez, Sergio. Hospital Universitario La Paz; España
Fil: Stringa, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Favaloro. Área de Investigación y Desarrollo; Argentina
Fil: Vallejo, Maria Teresa. Hospital Universitario La Paz; España
Fil: Largo, Carlota. Hospital Universitario La Paz; España
Fil: Encinas, Jose Luis. Hospital Universitario La Paz; España
Fil: Garcia de las Heras, Maria Soledad. Universidad Rey Juan Carlos; España
Fil: Lopez Santamaria, Manuel. Hospital Universitario La Paz; España
Fil: Tovar, Juan Antonio. Hospital Universitario La Paz; España
description Experimental small bowel transplantation (SBT) in rats has been proven to be a useful tool for the study of ischemia-reperfusion and immunological aspects related to solid organ transplantation. However, the model is not completely refined, specialized literature is scarce and complex technical details are typically omitted or confusing. Most studies related to acute rejection (AR) use the orthotopic standard, with small sample sizes due to its high mortality, whereas those studying chronic rejection (CR) use the heterotopic standard, which allows longer term survival but does not exactly reflect the human clinical scenario. Various animal strains have been used, and the type of rejection and the timing of its analysis differ among authors. The double purpose of this study was to develop an improved unusual AR model of SBT using the heterotopic technique, and to elaborate a guide useful to implement experimental models for studying AR. We analyzed the model's technical details and expected difficulties in overcoming the learning curve for such a complex microsurgical model, identifying the potential problem areas and providing a step-by-step protocol and reference guide for future surgeons interested in the topic. We also discuss the historic and more recent options in the literature.
publishDate 2016
dc.date.none.fl_str_mv 2016-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72902
Andres, Ane Miren; Santamaria, Monica; Hernandez Oliveros, Francisco; Guerra, Laura; Lopez, Sergio; et al.; Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation; Elsevier Science; Transplant Immunology; 36; 5-2016; 32-41
0966-3274
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72902
identifier_str_mv Andres, Ane Miren; Santamaria, Monica; Hernandez Oliveros, Francisco; Guerra, Laura; Lopez, Sergio; et al.; Difficulties, guidelines and review of developing an acute rejection model after rat intestinal transplantation; Elsevier Science; Transplant Immunology; 36; 5-2016; 32-41
0966-3274
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.trim.2016.04.003
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0966327416300156
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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