Ultrastructural responses of pancreatic β cells to metabolic alkalosis

Autores
Gómez Dumm, César Leandro Alberto; Rebolledo, Oscar Remigio; Gagliardino, Juan José
Año de publicación
1979
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The ultrastructural changes in pancreatic β cells were studied following glucose-induced insulin secretion in vitro, at two different extracellular pH (7.4 and 7.8). The pancreata perfused at pH 7.4 exhibited a biphasic insulin response to glucose challenge together with signs of increased emiocytotic activity and numerous microtubules in the β cells. Conversely, the pancreata perfused at pH 7.8 showed a significant decrease in insulin secretion, and their β cells revealed scarce emiocytotic images and a marked increase of intracellular granulolysis. These results represent the ultrastructural correlate of the reduced insulin secretion produced by metabolic alkalosis in the perfused rat pancreas.
Centro de Endocrinología Experimental y Aplicada
Materia
Biología
Ciencias Médicas
Insulin secretion
Electron microscopy
Acid-base changes
Pancreas perfusion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/145237
Acceder
id SEDICI_7e7cca459fbe1094a377568688e5f38c
oai_identifier_str oai:sedici.unlp.edu.ar:10915/145237
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Ultrastructural responses of pancreatic β cells to metabolic alkalosisGómez Dumm, César Leandro AlbertoRebolledo, Oscar RemigioGagliardino, Juan JoséBiologíaCiencias MédicasInsulin secretionElectron microscopyAcid-base changesPancreas perfusionThe ultrastructural changes in pancreatic β cells were studied following glucose-induced insulin secretion in vitro, at two different extracellular pH (7.4 and 7.8). The pancreata perfused at pH 7.4 exhibited a biphasic insulin response to glucose challenge together with signs of increased emiocytotic activity and numerous microtubules in the β cells. Conversely, the pancreata perfused at pH 7.8 showed a significant decrease in insulin secretion, and their β cells revealed scarce emiocytotic images and a marked increase of intracellular granulolysis. These results represent the ultrastructural correlate of the reduced insulin secretion produced by metabolic alkalosis in the perfused rat pancreas.Centro de Endocrinología Experimental y Aplicada1979-09-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf159-163http://sedici.unlp.edu.ar/handle/10915/145237enginfo:eu-repo/semantics/altIdentifier/issn/0302-766Xinfo:eu-repo/semantics/altIdentifier/issn/1432-0878info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00238055info:eu-repo/semantics/altIdentifier/pmid/43194info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:24:09Zoai:sedici.unlp.edu.ar:10915/145237Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:24:09.899SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Ultrastructural responses of pancreatic β cells to metabolic alkalosis
title Ultrastructural responses of pancreatic β cells to metabolic alkalosis
spellingShingle Ultrastructural responses of pancreatic β cells to metabolic alkalosis
Gómez Dumm, César Leandro Alberto
Biología
Ciencias Médicas
Insulin secretion
Electron microscopy
Acid-base changes
Pancreas perfusion
title_short Ultrastructural responses of pancreatic β cells to metabolic alkalosis
title_full Ultrastructural responses of pancreatic β cells to metabolic alkalosis
title_fullStr Ultrastructural responses of pancreatic β cells to metabolic alkalosis
title_full_unstemmed Ultrastructural responses of pancreatic β cells to metabolic alkalosis
title_sort Ultrastructural responses of pancreatic β cells to metabolic alkalosis
dc.creator.none.fl_str_mv Gómez Dumm, César Leandro Alberto
Rebolledo, Oscar Remigio
Gagliardino, Juan José
author Gómez Dumm, César Leandro Alberto
author_facet Gómez Dumm, César Leandro Alberto
Rebolledo, Oscar Remigio
Gagliardino, Juan José
author_role author
author2 Rebolledo, Oscar Remigio
Gagliardino, Juan José
author2_role author
author
dc.subject.none.fl_str_mv Biología
Ciencias Médicas
Insulin secretion
Electron microscopy
Acid-base changes
Pancreas perfusion
topic Biología
Ciencias Médicas
Insulin secretion
Electron microscopy
Acid-base changes
Pancreas perfusion
dc.description.none.fl_txt_mv The ultrastructural changes in pancreatic β cells were studied following glucose-induced insulin secretion in vitro, at two different extracellular pH (7.4 and 7.8). The pancreata perfused at pH 7.4 exhibited a biphasic insulin response to glucose challenge together with signs of increased emiocytotic activity and numerous microtubules in the β cells. Conversely, the pancreata perfused at pH 7.8 showed a significant decrease in insulin secretion, and their β cells revealed scarce emiocytotic images and a marked increase of intracellular granulolysis. These results represent the ultrastructural correlate of the reduced insulin secretion produced by metabolic alkalosis in the perfused rat pancreas.
Centro de Endocrinología Experimental y Aplicada
description The ultrastructural changes in pancreatic β cells were studied following glucose-induced insulin secretion in vitro, at two different extracellular pH (7.4 and 7.8). The pancreata perfused at pH 7.4 exhibited a biphasic insulin response to glucose challenge together with signs of increased emiocytotic activity and numerous microtubules in the β cells. Conversely, the pancreata perfused at pH 7.8 showed a significant decrease in insulin secretion, and their β cells revealed scarce emiocytotic images and a marked increase of intracellular granulolysis. These results represent the ultrastructural correlate of the reduced insulin secretion produced by metabolic alkalosis in the perfused rat pancreas.
publishDate 1979
dc.date.none.fl_str_mv 1979-09-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/145237
url http://sedici.unlp.edu.ar/handle/10915/145237
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0302-766X
info:eu-repo/semantics/altIdentifier/issn/1432-0878
info:eu-repo/semantics/altIdentifier/doi/10.1007/bf00238055
info:eu-repo/semantics/altIdentifier/pmid/43194
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
159-163
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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