Vectorial insulin secretion by pancreatic β-cells
- Autores
- Cortizo, Ana María; Espinal, Joseph; Hammonds, Peter
- Año de publicación
- 1990
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Morphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture system was verified in the presence of confluent HIT-T15 cells. When stimulated with sulphonylurea, ionophore or high potassium, insulin was preferentially released into the lower chamber irrespective of whether secretagogues were added to the upper or lower chambers. Vectorial insulin secretion may be a significant determinant of islet hormone paracrine interactions in the maintenance of glucose homeostasis.
Facultad de Ciencias Médicas - Materia
-
Ciencias Médicas
Insulina
Páncreas - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/67399
Ver los metadatos del registro completo
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Vectorial insulin secretion by pancreatic β-cellsCortizo, Ana MaríaEspinal, JosephHammonds, PeterCiencias MédicasInsulinaPáncreasMorphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture system was verified in the presence of confluent HIT-T15 cells. When stimulated with sulphonylurea, ionophore or high potassium, insulin was preferentially released into the lower chamber irrespective of whether secretagogues were added to the upper or lower chambers. Vectorial insulin secretion may be a significant determinant of islet hormone paracrine interactions in the maintenance of glucose homeostasis.Facultad de Ciencias Médicas1990info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf137-140http://sedici.unlp.edu.ar/handle/10915/67399enginfo:eu-repo/semantics/altIdentifier/hdl/11746/4449info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T09:53:07Zoai:sedici.unlp.edu.ar:10915/67399Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 09:53:08.19SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
Vectorial insulin secretion by pancreatic β-cells |
| title |
Vectorial insulin secretion by pancreatic β-cells |
| spellingShingle |
Vectorial insulin secretion by pancreatic β-cells Cortizo, Ana María Ciencias Médicas Insulina Páncreas |
| title_short |
Vectorial insulin secretion by pancreatic β-cells |
| title_full |
Vectorial insulin secretion by pancreatic β-cells |
| title_fullStr |
Vectorial insulin secretion by pancreatic β-cells |
| title_full_unstemmed |
Vectorial insulin secretion by pancreatic β-cells |
| title_sort |
Vectorial insulin secretion by pancreatic β-cells |
| dc.creator.none.fl_str_mv |
Cortizo, Ana María Espinal, Joseph Hammonds, Peter |
| author |
Cortizo, Ana María |
| author_facet |
Cortizo, Ana María Espinal, Joseph Hammonds, Peter |
| author_role |
author |
| author2 |
Espinal, Joseph Hammonds, Peter |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Ciencias Médicas Insulina Páncreas |
| topic |
Ciencias Médicas Insulina Páncreas |
| dc.description.none.fl_txt_mv |
Morphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture system was verified in the presence of confluent HIT-T15 cells. When stimulated with sulphonylurea, ionophore or high potassium, insulin was preferentially released into the lower chamber irrespective of whether secretagogues were added to the upper or lower chambers. Vectorial insulin secretion may be a significant determinant of islet hormone paracrine interactions in the maintenance of glucose homeostasis. Facultad de Ciencias Médicas |
| description |
Morphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture system was verified in the presence of confluent HIT-T15 cells. When stimulated with sulphonylurea, ionophore or high potassium, insulin was preferentially released into the lower chamber irrespective of whether secretagogues were added to the upper or lower chambers. Vectorial insulin secretion may be a significant determinant of islet hormone paracrine interactions in the maintenance of glucose homeostasis. |
| publishDate |
1990 |
| dc.date.none.fl_str_mv |
1990 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Articulo http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://sedici.unlp.edu.ar/handle/10915/67399 |
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http://sedici.unlp.edu.ar/handle/10915/67399 |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
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info:eu-repo/semantics/altIdentifier/hdl/11746/4449 |
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info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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openAccess |
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http://creativecommons.org/licenses/by/4.0/ Creative Commons Attribution 4.0 International (CC BY 4.0) |
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application/pdf 137-140 |
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