Vectorial insulin secretion by pancreatic β-cells

Autores
Cortizo, Ana María; Espinal, Joseph; Hammonds, Peter
Año de publicación
1990
Idioma
inglés
Tipo de recurso
artículo
Estado
versión enviada
Descripción
Morphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture system was verified in the presence of confluent HIT-T15 cells. When stimulated with sulphonylurea, ionophore or high potassium, insulin was preferentially released into the lower chamber irrespective of whether secretagogues were added to the upper or lower chambers. Vectorial insulin secretion may be a significant determinant of islet hormone paracrine interactions in the maintenance of glucose homeostasis.
Materia
Ciencias Químicas
Epithelial polarity
HIT-T15 β-cell/Insulin secretion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
CIC Digital (CICBA)
Institución
Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
OAI Identificador
oai:digital.cic.gba.gob.ar:11746/4449
Acceder
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oai_identifier_str oai:digital.cic.gba.gob.ar:11746/4449
network_acronym_str CICBA
repository_id_str 9441
network_name_str CIC Digital (CICBA)
spelling Vectorial insulin secretion by pancreatic β-cellsCortizo, Ana MaríaEspinal, JosephHammonds, PeterCiencias QuímicasEpithelial polarityHIT-T15 β-cell/Insulin secretionMorphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture system was verified in the presence of confluent HIT-T15 cells. When stimulated with sulphonylurea, ionophore or high potassium, insulin was preferentially released into the lower chamber irrespective of whether secretagogues were added to the upper or lower chambers. Vectorial insulin secretion may be a significant determinant of islet hormone paracrine interactions in the maintenance of glucose homeostasis.1990info:eu-repo/semantics/articleinfo:eu-repo/semantics/submittedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://digital.cic.gba.gob.ar/handle/11746/4449enginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/reponame:CIC Digital (CICBA)instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Airesinstacron:CICBA2025-10-16T09:27:04Zoai:digital.cic.gba.gob.ar:11746/4449Institucionalhttp://digital.cic.gba.gob.arOrganismo científico-tecnológicoNo correspondehttp://digital.cic.gba.gob.ar/oai/snrdmarisa.degiusti@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:94412025-10-16 09:27:04.255CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Airesfalse
dc.title.none.fl_str_mv Vectorial insulin secretion by pancreatic β-cells
title Vectorial insulin secretion by pancreatic β-cells
spellingShingle Vectorial insulin secretion by pancreatic β-cells
Cortizo, Ana María
Ciencias Químicas
Epithelial polarity
HIT-T15 β-cell/Insulin secretion
title_short Vectorial insulin secretion by pancreatic β-cells
title_full Vectorial insulin secretion by pancreatic β-cells
title_fullStr Vectorial insulin secretion by pancreatic β-cells
title_full_unstemmed Vectorial insulin secretion by pancreatic β-cells
title_sort Vectorial insulin secretion by pancreatic β-cells
dc.creator.none.fl_str_mv Cortizo, Ana María
Espinal, Joseph
Hammonds, Peter
author Cortizo, Ana María
author_facet Cortizo, Ana María
Espinal, Joseph
Hammonds, Peter
author_role author
author2 Espinal, Joseph
Hammonds, Peter
author2_role author
author
dc.subject.none.fl_str_mv Ciencias Químicas
Epithelial polarity
HIT-T15 β-cell/Insulin secretion
topic Ciencias Químicas
Epithelial polarity
HIT-T15 β-cell/Insulin secretion
dc.description.none.fl_txt_mv Morphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture system was verified in the presence of confluent HIT-T15 cells. When stimulated with sulphonylurea, ionophore or high potassium, insulin was preferentially released into the lower chamber irrespective of whether secretagogues were added to the upper or lower chambers. Vectorial insulin secretion may be a significant determinant of islet hormone paracrine interactions in the maintenance of glucose homeostasis.
description Morphological studies of pancreatic β-cells have suggested the presence of discrete sensory and secretory domains. In the present study we now provide functional evidence by demonstrating polarity of insulin release by HIT-T15 cells. A significant diffusion barrier across a twin chamber culture system was verified in the presence of confluent HIT-T15 cells. When stimulated with sulphonylurea, ionophore or high potassium, insulin was preferentially released into the lower chamber irrespective of whether secretagogues were added to the upper or lower chambers. Vectorial insulin secretion may be a significant determinant of islet hormone paracrine interactions in the maintenance of glucose homeostasis.
publishDate 1990
dc.date.none.fl_str_mv 1990
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv https://digital.cic.gba.gob.ar/handle/11746/4449
url https://digital.cic.gba.gob.ar/handle/11746/4449
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:CIC Digital (CICBA)
instname:Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron:CICBA
reponame_str CIC Digital (CICBA)
collection CIC Digital (CICBA)
instname_str Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
instacron_str CICBA
institution CICBA
repository.name.fl_str_mv CIC Digital (CICBA) - Comisión de Investigaciones Científicas de la Provincia de Buenos Aires
repository.mail.fl_str_mv marisa.degiusti@sedici.unlp.edu.ar
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score 12.712165

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