A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i>
- Autores
- Draghi, Walter Omar; Del Papa, María Florencia; Hellweg, C.; Watt, S. A.; Watt, T. F.; Barsch, A.; Lozano, Mauricio Javier; Lagares, A.; Salas, María Eugenia; López, José Luis; Albicoro, Francisco Javier; Nilsson, Juliet Fernanda; Torres Tejerizo, Gonzalo Arturo; Luna, María Flavia; Pistorio, Mariano; Boiardi, José Luis; Pühler, A.; Weidner, S.; Niehaus, K.; Lagares, Antonio
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiologic changes that occur in acid-stressed bacteria. We present here an integrated analysis entailing the main cultural, metabolic, and molecular responses of the model bacterium Sinorhizobium meliloti growing under controlled acid stress in a chemostat. A stepwise extracellular acidification of the culture medium had indicated that S. meliloti stopped growing at ca. pH 6.0-6.1. Under such stress the rhizobia increased the O2 consumption per cell by more than 5-fold. This phenotype, together with an increase in the transcripts for several membrane cytochromes, entails a higher aerobic-respiration rate in the acid-stressed rhizobia. Multivariate analysis of global metabolome data served to unequivocally correlate specific-metabolite profiles with the extracellular pH, showing that at low pH the pentose-phosphate pathway exhibited increases in several transcripts, enzymes, and metabolites. Further analyses should be focused on the time course of the observed changes, its associated intracellular signaling, and on the comparison with the changes that operate during the sub lethal acid-adaptive response (ATR) in rhizobia.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Instituto de Biotecnologia y Biologia Molecular - Materia
-
Ciencias Exactas
Sinorhizobium meliloti - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by/4.0/
- Repositorio
.jpg)
- Institución
- Universidad Nacional de La Plata
- OAI Identificador
- oai:sedici.unlp.edu.ar:10915/86492
Ver los metadatos del registro completo
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A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i>Draghi, Walter OmarDel Papa, María FlorenciaHellweg, C.Watt, S. A.Watt, T. F.Barsch, A.Lozano, Mauricio JavierLagares, A.Salas, María EugeniaLópez, José LuisAlbicoro, Francisco JavierNilsson, Juliet FernandaTorres Tejerizo, Gonzalo ArturoLuna, María FlaviaPistorio, MarianoBoiardi, José LuisPühler, A.Weidner, S.Niehaus, K.Lagares, AntonioCiencias ExactasSinorhizobium melilotiAbiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiologic changes that occur in acid-stressed bacteria. We present here an integrated analysis entailing the main cultural, metabolic, and molecular responses of the model bacterium <i>Sinorhizobium meliloti</i> growing under controlled acid stress in a chemostat. A stepwise extracellular acidification of the culture medium had indicated that <i>S. meliloti</i> stopped growing at ca. pH 6.0-6.1. Under such stress the rhizobia increased the O<SUB>2</SUB> consumption per cell by more than 5-fold. This phenotype, together with an increase in the transcripts for several membrane cytochromes, entails a higher aerobic-respiration rate in the acid-stressed rhizobia. Multivariate analysis of global metabolome data served to unequivocally correlate specific-metabolite profiles with the extracellular pH, showing that at low pH the pentose-phosphate pathway exhibited increases in several transcripts, enzymes, and metabolites. Further analyses should be focused on the time course of the observed changes, its associated intracellular signaling, and on the comparison with the changes that operate during the sub lethal acid-adaptive response (ATR) in rhizobia.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Fermentaciones IndustrialesInstituto de Biotecnologia y Biologia Molecular2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/86492enginfo:eu-repo/semantics/altIdentifier/issn/2045-2322info:eu-repo/semantics/altIdentifier/doi/10.1038/srep29278info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-03T10:49:03Zoai:sedici.unlp.edu.ar:10915/86492Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-03 10:49:03.904SEDICI (UNLP) - Universidad Nacional de La Platafalse |
| dc.title.none.fl_str_mv |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| spellingShingle |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> Draghi, Walter Omar Ciencias Exactas Sinorhizobium meliloti |
| title_short |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title_full |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title_fullStr |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title_full_unstemmed |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| title_sort |
A consolidated analysis of the physiologic and molecular responses induced under acid stress in the legume-symbiont model-soil bacterium <i>Sinorhizobium meliloti</i> |
| dc.creator.none.fl_str_mv |
Draghi, Walter Omar Del Papa, María Florencia Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, Mauricio Javier Lagares, A. Salas, María Eugenia López, José Luis Albicoro, Francisco Javier Nilsson, Juliet Fernanda Torres Tejerizo, Gonzalo Arturo Luna, María Flavia Pistorio, Mariano Boiardi, José Luis Pühler, A. Weidner, S. Niehaus, K. Lagares, Antonio |
| author |
Draghi, Walter Omar |
| author_facet |
Draghi, Walter Omar Del Papa, María Florencia Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, Mauricio Javier Lagares, A. Salas, María Eugenia López, José Luis Albicoro, Francisco Javier Nilsson, Juliet Fernanda Torres Tejerizo, Gonzalo Arturo Luna, María Flavia Pistorio, Mariano Boiardi, José Luis Pühler, A. Weidner, S. Niehaus, K. Lagares, Antonio |
| author_role |
author |
| author2 |
Del Papa, María Florencia Hellweg, C. Watt, S. A. Watt, T. F. Barsch, A. Lozano, Mauricio Javier Lagares, A. Salas, María Eugenia López, José Luis Albicoro, Francisco Javier Nilsson, Juliet Fernanda Torres Tejerizo, Gonzalo Arturo Luna, María Flavia Pistorio, Mariano Boiardi, José Luis Pühler, A. Weidner, S. Niehaus, K. Lagares, Antonio |
| author2_role |
author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Ciencias Exactas Sinorhizobium meliloti |
| topic |
Ciencias Exactas Sinorhizobium meliloti |
| dc.description.none.fl_txt_mv |
Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiologic changes that occur in acid-stressed bacteria. We present here an integrated analysis entailing the main cultural, metabolic, and molecular responses of the model bacterium <i>Sinorhizobium meliloti</i> growing under controlled acid stress in a chemostat. A stepwise extracellular acidification of the culture medium had indicated that <i>S. meliloti</i> stopped growing at ca. pH 6.0-6.1. Under such stress the rhizobia increased the O<SUB>2</SUB> consumption per cell by more than 5-fold. This phenotype, together with an increase in the transcripts for several membrane cytochromes, entails a higher aerobic-respiration rate in the acid-stressed rhizobia. Multivariate analysis of global metabolome data served to unequivocally correlate specific-metabolite profiles with the extracellular pH, showing that at low pH the pentose-phosphate pathway exhibited increases in several transcripts, enzymes, and metabolites. Further analyses should be focused on the time course of the observed changes, its associated intracellular signaling, and on the comparison with the changes that operate during the sub lethal acid-adaptive response (ATR) in rhizobia. Facultad de Ciencias Exactas Centro de Investigación y Desarrollo en Fermentaciones Industriales Instituto de Biotecnologia y Biologia Molecular |
| description |
Abiotic stresses in general and extracellular acidity in particular disturb and limit nitrogen-fixing symbioses between rhizobia and their host legumes. Except for valuable molecular-biological studies on different rhizobia, no consolidated models have been formulated to describe the central physiologic changes that occur in acid-stressed bacteria. We present here an integrated analysis entailing the main cultural, metabolic, and molecular responses of the model bacterium <i>Sinorhizobium meliloti</i> growing under controlled acid stress in a chemostat. A stepwise extracellular acidification of the culture medium had indicated that <i>S. meliloti</i> stopped growing at ca. pH 6.0-6.1. Under such stress the rhizobia increased the O<SUB>2</SUB> consumption per cell by more than 5-fold. This phenotype, together with an increase in the transcripts for several membrane cytochromes, entails a higher aerobic-respiration rate in the acid-stressed rhizobia. Multivariate analysis of global metabolome data served to unequivocally correlate specific-metabolite profiles with the extracellular pH, showing that at low pH the pentose-phosphate pathway exhibited increases in several transcripts, enzymes, and metabolites. Further analyses should be focused on the time course of the observed changes, its associated intracellular signaling, and on the comparison with the changes that operate during the sub lethal acid-adaptive response (ATR) in rhizobia. |
| publishDate |
2016 |
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2016 |
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eng |
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eng |
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info:eu-repo/semantics/altIdentifier/issn/2045-2322 info:eu-repo/semantics/altIdentifier/doi/10.1038/srep29278 |
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