Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation

Autores
Rinaudi, Luciana Veronica; Fujishige, Nancy A.; Hirsch, Ann M.; Banchio, Erika; Zorreguieta, Ángeles; Giordano, Walter Fabian
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Rhizobia are non-spore-forming soil bacteria that fix atmospheric nitrogen into ammonia in a symbiosis with legume roots. However, in the absence of a legume host, rhizobia manage to survive and hence must have evolved strategies to adapt to diverse environmental conditions. The capacity to respond to variations in nutrient availability enables the persistence of rhizobial species in soil, and consequently improves their ability to colonize and to survive in the host plant. Rhizobia, like many other soil bacteria, persist in nature most likely in sessile communities known as biofilms, which are most often composed of multiple microbial species. We have been employing in vitro assays to study environmental parameters that might influence biofilm formation in the Medicago symbiont Sinorhizobium meliloti. These parameters include carbon source, amount of nitrate, phosphate, calcium and magnesium as well as the effects of osmolarity and pH. The microtiter plate assay facilitates the detection of subtle differences in rhizobial biofilms in response to these parameters, thereby providing insight into how environmental stress or nutritional status influences rhizobial survival. Nutrients such as sucrose, phosphate and calcium enhance biofilm formation as their concentrations increase, whereas extreme temperatures and pH negatively affect biofilm formation.
Fil: Rinaudi, Luciana Veronica. Universidad Nacional de Río Cuarto; Argentina
Fil: Fujishige, Nancy A.. University of California; Estados Unidos
Fil: Hirsch, Ann M.. University of California; Estados Unidos
Fil: Banchio, Erika. Universidad Nacional de Río Cuarto; Argentina
Fil: Zorreguieta, Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto; Argentina
Materia
Biofilm
Environmental Signals
Sinorhizobium Meliloti
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/39406
Acceder
id CONICETDig_6faacf14b3a2193d26c350fe1e356c3a
oai_identifier_str oai:ri.conicet.gov.ar:11336/39406
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formationRinaudi, Luciana VeronicaFujishige, Nancy A.Hirsch, Ann M.Banchio, ErikaZorreguieta, ÁngelesGiordano, Walter FabianBiofilmEnvironmental SignalsSinorhizobium Melilotihttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Rhizobia are non-spore-forming soil bacteria that fix atmospheric nitrogen into ammonia in a symbiosis with legume roots. However, in the absence of a legume host, rhizobia manage to survive and hence must have evolved strategies to adapt to diverse environmental conditions. The capacity to respond to variations in nutrient availability enables the persistence of rhizobial species in soil, and consequently improves their ability to colonize and to survive in the host plant. Rhizobia, like many other soil bacteria, persist in nature most likely in sessile communities known as biofilms, which are most often composed of multiple microbial species. We have been employing in vitro assays to study environmental parameters that might influence biofilm formation in the Medicago symbiont Sinorhizobium meliloti. These parameters include carbon source, amount of nitrate, phosphate, calcium and magnesium as well as the effects of osmolarity and pH. The microtiter plate assay facilitates the detection of subtle differences in rhizobial biofilms in response to these parameters, thereby providing insight into how environmental stress or nutritional status influences rhizobial survival. Nutrients such as sucrose, phosphate and calcium enhance biofilm formation as their concentrations increase, whereas extreme temperatures and pH negatively affect biofilm formation.Fil: Rinaudi, Luciana Veronica. Universidad Nacional de Río Cuarto; ArgentinaFil: Fujishige, Nancy A.. University of California; Estados UnidosFil: Hirsch, Ann M.. University of California; Estados UnidosFil: Banchio, Erika. Universidad Nacional de Río Cuarto; ArgentinaFil: Zorreguieta, Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; ArgentinaFil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto; ArgentinaElsevier Science2006-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/39406Rinaudi, Luciana Veronica; Fujishige, Nancy A.; Hirsch, Ann M.; Banchio, Erika; Zorreguieta, Ángeles; et al.; Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation; Elsevier Science; Research In Microbiology; 157; 9; 11-2006; 867-8750923-25081769-7123CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0923250806001264info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2006.06.002info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:48:49Zoai:ri.conicet.gov.ar:11336/39406instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:48:49.914CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation
title Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation
spellingShingle Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation
Rinaudi, Luciana Veronica
Biofilm
Environmental Signals
Sinorhizobium Meliloti
title_short Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation
title_full Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation
title_fullStr Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation
title_full_unstemmed Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation
title_sort Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation
dc.creator.none.fl_str_mv Rinaudi, Luciana Veronica
Fujishige, Nancy A.
Hirsch, Ann M.
Banchio, Erika
Zorreguieta, Ángeles
Giordano, Walter Fabian
author Rinaudi, Luciana Veronica
author_facet Rinaudi, Luciana Veronica
Fujishige, Nancy A.
Hirsch, Ann M.
Banchio, Erika
Zorreguieta, Ángeles
Giordano, Walter Fabian
author_role author
author2 Fujishige, Nancy A.
Hirsch, Ann M.
Banchio, Erika
Zorreguieta, Ángeles
Giordano, Walter Fabian
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Biofilm
Environmental Signals
Sinorhizobium Meliloti
topic Biofilm
Environmental Signals
Sinorhizobium Meliloti
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Rhizobia are non-spore-forming soil bacteria that fix atmospheric nitrogen into ammonia in a symbiosis with legume roots. However, in the absence of a legume host, rhizobia manage to survive and hence must have evolved strategies to adapt to diverse environmental conditions. The capacity to respond to variations in nutrient availability enables the persistence of rhizobial species in soil, and consequently improves their ability to colonize and to survive in the host plant. Rhizobia, like many other soil bacteria, persist in nature most likely in sessile communities known as biofilms, which are most often composed of multiple microbial species. We have been employing in vitro assays to study environmental parameters that might influence biofilm formation in the Medicago symbiont Sinorhizobium meliloti. These parameters include carbon source, amount of nitrate, phosphate, calcium and magnesium as well as the effects of osmolarity and pH. The microtiter plate assay facilitates the detection of subtle differences in rhizobial biofilms in response to these parameters, thereby providing insight into how environmental stress or nutritional status influences rhizobial survival. Nutrients such as sucrose, phosphate and calcium enhance biofilm formation as their concentrations increase, whereas extreme temperatures and pH negatively affect biofilm formation.
Fil: Rinaudi, Luciana Veronica. Universidad Nacional de Río Cuarto; Argentina
Fil: Fujishige, Nancy A.. University of California; Estados Unidos
Fil: Hirsch, Ann M.. University of California; Estados Unidos
Fil: Banchio, Erika. Universidad Nacional de Río Cuarto; Argentina
Fil: Zorreguieta, Ángeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
Fil: Giordano, Walter Fabian. Universidad Nacional de Río Cuarto; Argentina
description Rhizobia are non-spore-forming soil bacteria that fix atmospheric nitrogen into ammonia in a symbiosis with legume roots. However, in the absence of a legume host, rhizobia manage to survive and hence must have evolved strategies to adapt to diverse environmental conditions. The capacity to respond to variations in nutrient availability enables the persistence of rhizobial species in soil, and consequently improves their ability to colonize and to survive in the host plant. Rhizobia, like many other soil bacteria, persist in nature most likely in sessile communities known as biofilms, which are most often composed of multiple microbial species. We have been employing in vitro assays to study environmental parameters that might influence biofilm formation in the Medicago symbiont Sinorhizobium meliloti. These parameters include carbon source, amount of nitrate, phosphate, calcium and magnesium as well as the effects of osmolarity and pH. The microtiter plate assay facilitates the detection of subtle differences in rhizobial biofilms in response to these parameters, thereby providing insight into how environmental stress or nutritional status influences rhizobial survival. Nutrients such as sucrose, phosphate and calcium enhance biofilm formation as their concentrations increase, whereas extreme temperatures and pH negatively affect biofilm formation.
publishDate 2006
dc.date.none.fl_str_mv 2006-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/39406
Rinaudi, Luciana Veronica; Fujishige, Nancy A.; Hirsch, Ann M.; Banchio, Erika; Zorreguieta, Ángeles; et al.; Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation; Elsevier Science; Research In Microbiology; 157; 9; 11-2006; 867-875
0923-2508
1769-7123
CONICET Digital
CONICET
url http://hdl.handle.net/11336/39406
identifier_str_mv Rinaudi, Luciana Veronica; Fujishige, Nancy A.; Hirsch, Ann M.; Banchio, Erika; Zorreguieta, Ángeles; et al.; Effects of nutritional and environmental conditions on Sinorhizobium meliloti biofilm formation; Elsevier Science; Research In Microbiology; 157; 9; 11-2006; 867-875
0923-2508
1769-7123
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0923250806001264
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.resmic.2006.06.002
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268942074118144
score 13.13397

Publicaciones similares