Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo

Autores
Humen, Martín Andrés; De Antoni, Graciela Liliana; Benyacoub, Jalil; Costas, María Elena; Cardozo, Marta; Kozubsky, Leonora Eugenia; Saudan, Kim Yen; Boenzli Bruand, Angele; Blum, Stephanie; Schiffrin, Eduardo J.; Pérez, Pablo Fernando
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This study describes the in vivo activity of Lactobacillus johnsonii La1 (NCC533) in Giardia intestinalis-infected gerbils (Meriones unguiculatus). Daily administration of lactobacilli in the drinking water from 7 days before inoculation with Giardia trophozoites efficiently prevented G. intestinalis strain WB clone C6 from infecting gerbils. More specifically, shedding of fecal Giardia antigens (GSA65 protein) was diminished in the La1-treated group, and resolution of infection was observed by 21 days postinoculation. Histology and analysis of enzymatic markers of microvillus membrane integrity revealed that probiotic administration also protected against parasite-induced mucosal damage. In addition, a cellular response to Giardia antigens was stimulated in spleen cells from La1-treated gerbils. Results show for the first time the antigiardial effect of probiotic lactobacilli in vivo and provide further insight into the antagonistic properties of lactic acid bacteria against protozoa involved in intestinal infections.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Ciencias Exactas
Lactobacillus johnsonii
Giardia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/83266
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/83266
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivoHumen, Martín AndrésDe Antoni, Graciela LilianaBenyacoub, JalilCostas, María ElenaCardozo, MartaKozubsky, Leonora EugeniaSaudan, Kim YenBoenzli Bruand, AngeleBlum, StephanieSchiffrin, Eduardo J.Pérez, Pablo FernandoCiencias ExactasLactobacillus johnsoniiGiardiaThis study describes the in vivo activity of Lactobacillus johnsonii La1 (NCC533) in Giardia intestinalis-infected gerbils (Meriones unguiculatus). Daily administration of lactobacilli in the drinking water from 7 days before inoculation with Giardia trophozoites efficiently prevented G. intestinalis strain WB clone C6 from infecting gerbils. More specifically, shedding of fecal Giardia antigens (GSA65 protein) was diminished in the La1-treated group, and resolution of infection was observed by 21 days postinoculation. Histology and analysis of enzymatic markers of microvillus membrane integrity revealed that probiotic administration also protected against parasite-induced mucosal damage. In addition, a cellular response to Giardia antigens was stimulated in spleen cells from La1-treated gerbils. Results show for the first time the antigiardial effect of probiotic lactobacilli in vivo and provide further insight into the antagonistic properties of lactic acid bacteria against protozoa involved in intestinal infections.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Criotecnología de Alimentos2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1265-1269http://sedici.unlp.edu.ar/handle/10915/83266enginfo:eu-repo/semantics/altIdentifier/issn/0019-9567info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.73.2.1265-1269.2005info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-12T10:40:04Zoai:sedici.unlp.edu.ar:10915/83266Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-12 10:40:04.593SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo
title Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo
spellingShingle Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo
Humen, Martín Andrés
Ciencias Exactas
Lactobacillus johnsonii
Giardia
title_short Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo
title_full Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo
title_fullStr Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo
title_full_unstemmed Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo
title_sort Lactobacillus johnsonii La1 antagonizes Giardia intestinalis in vivo
dc.creator.none.fl_str_mv Humen, Martín Andrés
De Antoni, Graciela Liliana
Benyacoub, Jalil
Costas, María Elena
Cardozo, Marta
Kozubsky, Leonora Eugenia
Saudan, Kim Yen
Boenzli Bruand, Angele
Blum, Stephanie
Schiffrin, Eduardo J.
Pérez, Pablo Fernando
author Humen, Martín Andrés
author_facet Humen, Martín Andrés
De Antoni, Graciela Liliana
Benyacoub, Jalil
Costas, María Elena
Cardozo, Marta
Kozubsky, Leonora Eugenia
Saudan, Kim Yen
Boenzli Bruand, Angele
Blum, Stephanie
Schiffrin, Eduardo J.
Pérez, Pablo Fernando
author_role author
author2 De Antoni, Graciela Liliana
Benyacoub, Jalil
Costas, María Elena
Cardozo, Marta
Kozubsky, Leonora Eugenia
Saudan, Kim Yen
Boenzli Bruand, Angele
Blum, Stephanie
Schiffrin, Eduardo J.
Pérez, Pablo Fernando
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Lactobacillus johnsonii
Giardia
topic Ciencias Exactas
Lactobacillus johnsonii
Giardia
dc.description.none.fl_txt_mv This study describes the in vivo activity of Lactobacillus johnsonii La1 (NCC533) in Giardia intestinalis-infected gerbils (Meriones unguiculatus). Daily administration of lactobacilli in the drinking water from 7 days before inoculation with Giardia trophozoites efficiently prevented G. intestinalis strain WB clone C6 from infecting gerbils. More specifically, shedding of fecal Giardia antigens (GSA65 protein) was diminished in the La1-treated group, and resolution of infection was observed by 21 days postinoculation. Histology and analysis of enzymatic markers of microvillus membrane integrity revealed that probiotic administration also protected against parasite-induced mucosal damage. In addition, a cellular response to Giardia antigens was stimulated in spleen cells from La1-treated gerbils. Results show for the first time the antigiardial effect of probiotic lactobacilli in vivo and provide further insight into the antagonistic properties of lactic acid bacteria against protozoa involved in intestinal infections.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description This study describes the in vivo activity of Lactobacillus johnsonii La1 (NCC533) in Giardia intestinalis-infected gerbils (Meriones unguiculatus). Daily administration of lactobacilli in the drinking water from 7 days before inoculation with Giardia trophozoites efficiently prevented G. intestinalis strain WB clone C6 from infecting gerbils. More specifically, shedding of fecal Giardia antigens (GSA65 protein) was diminished in the La1-treated group, and resolution of infection was observed by 21 days postinoculation. Histology and analysis of enzymatic markers of microvillus membrane integrity revealed that probiotic administration also protected against parasite-induced mucosal damage. In addition, a cellular response to Giardia antigens was stimulated in spleen cells from La1-treated gerbils. Results show for the first time the antigiardial effect of probiotic lactobacilli in vivo and provide further insight into the antagonistic properties of lactic acid bacteria against protozoa involved in intestinal infections.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/83266
url http://sedici.unlp.edu.ar/handle/10915/83266
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0019-9567
info:eu-repo/semantics/altIdentifier/doi/10.1128/IAI.73.2.1265-1269.2005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
1265-1269
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.24909

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