Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study

Autores
Pérez, Pablo Fernando; Minnaard, Jessica; Rouvet, Martine; Knabenhans, Christian; Brassart, Dominique; De Antoni, Graciela Liliana; Schiffrin, Eduardo J.
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the present work was to evaluate the effect of spent culture supernatants of different strains of lactobacilli on giardia trophozoites. The growth of Giardia intestinalis strain WB, as well as the attachment to the human intestinal epithelial cell line Caco-2, was evaluated by using proliferation and adhesion assays with radiolabeled parasites. In addition, scanning electron microscopy and flow cytometric analysis were performed. The effect of spent culture supernatants from lactobacilli was strain dependent. Lactobacillus johnsonii La1 significantly inhibited the proliferation of G. intestinalis trophozoites. Although the effect was strongly pH dependent, it was not simply due to lactic acid. According to flow cytometric analysis, trophozoites were arrested in G1 phase but neither significant necrosis nor apoptosis could be detected. Bacterial cells or their spent culture supernatants were unable to modify trophozoite attachment to Caco-2 cells. However, trophozoites treated with spent culture supernatants had little, if any, proliferative capacity. These results suggest that La1 produces some substance(s) able to inhibit proliferation of Giardia trophozoites. Partial characterization of the factors involved in the antigiardiasic action showed that they have a low molecular mass and are inactivated by heating. On this basis, it seems worthwhile to explore how colonization of the proximal small bowel with these lactic acid bacteria could interfere with giardiasis in vivo.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Materia
Ciencias Exactas
Giardia intestinalis
in vitro study
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/107655
Acceder
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network_name_str SEDICI (UNLP)
spelling Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro studyPérez, Pablo FernandoMinnaard, JessicaRouvet, MartineKnabenhans, ChristianBrassart, DominiqueDe Antoni, Graciela LilianaSchiffrin, Eduardo J.Ciencias ExactasGiardia intestinalisin vitro studyThe aim of the present work was to evaluate the effect of spent culture supernatants of different strains of lactobacilli on giardia trophozoites. The growth of <i>Giardia intestinalis</i> strain WB, as well as the attachment to the human intestinal epithelial cell line Caco-2, was evaluated by using proliferation and adhesion assays with radiolabeled parasites. In addition, scanning electron microscopy and flow cytometric analysis were performed. The effect of spent culture supernatants from lactobacilli was strain dependent. <i>Lactobacillus johnsonii</i> La1 significantly inhibited the proliferation of <i>G. intestinalis</i> trophozoites. Although the effect was strongly pH dependent, it was not simply due to lactic acid. According to flow cytometric analysis, trophozoites were arrested in G<sub>1</sub> phase but neither significant necrosis nor apoptosis could be detected. Bacterial cells or their spent culture supernatants were unable to modify trophozoite attachment to Caco-2 cells. However, trophozoites treated with spent culture supernatants had little, if any, proliferative capacity. These results suggest that La1 produces some substance(s) able to inhibit proliferation of <i>Giardia</i> trophozoites. Partial characterization of the factors involved in the antigiardiasic action showed that they have a low molecular mass and are inactivated by heating. On this basis, it seems worthwhile to explore how colonization of the proximal small bowel with these lactic acid bacteria could interfere with giardiasis <i>in vivo</i>.Facultad de Ciencias ExactasCentro de Investigación y Desarrollo en Criotecnología de Alimentos2001info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf5037-5042http://sedici.unlp.edu.ar/handle/10915/107655enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC93268&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/67/11/5037info:eu-repo/semantics/altIdentifier/issn/0099-2240info:eu-repo/semantics/altIdentifier/pmid/11679323info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T10:06:40Zoai:sedici.unlp.edu.ar:10915/107655Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 10:06:40.361SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
spellingShingle Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
Pérez, Pablo Fernando
Ciencias Exactas
Giardia intestinalis
in vitro study
title_short Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title_full Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title_fullStr Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title_full_unstemmed Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
title_sort Inhibition of <i>Giardia intestinalis</i> by extracellular factors from Lactobacilli: an in vitro study
dc.creator.none.fl_str_mv Pérez, Pablo Fernando
Minnaard, Jessica
Rouvet, Martine
Knabenhans, Christian
Brassart, Dominique
De Antoni, Graciela Liliana
Schiffrin, Eduardo J.
author Pérez, Pablo Fernando
author_facet Pérez, Pablo Fernando
Minnaard, Jessica
Rouvet, Martine
Knabenhans, Christian
Brassart, Dominique
De Antoni, Graciela Liliana
Schiffrin, Eduardo J.
author_role author
author2 Minnaard, Jessica
Rouvet, Martine
Knabenhans, Christian
Brassart, Dominique
De Antoni, Graciela Liliana
Schiffrin, Eduardo J.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Ciencias Exactas
Giardia intestinalis
in vitro study
topic Ciencias Exactas
Giardia intestinalis
in vitro study
dc.description.none.fl_txt_mv The aim of the present work was to evaluate the effect of spent culture supernatants of different strains of lactobacilli on giardia trophozoites. The growth of <i>Giardia intestinalis</i> strain WB, as well as the attachment to the human intestinal epithelial cell line Caco-2, was evaluated by using proliferation and adhesion assays with radiolabeled parasites. In addition, scanning electron microscopy and flow cytometric analysis were performed. The effect of spent culture supernatants from lactobacilli was strain dependent. <i>Lactobacillus johnsonii</i> La1 significantly inhibited the proliferation of <i>G. intestinalis</i> trophozoites. Although the effect was strongly pH dependent, it was not simply due to lactic acid. According to flow cytometric analysis, trophozoites were arrested in G<sub>1</sub> phase but neither significant necrosis nor apoptosis could be detected. Bacterial cells or their spent culture supernatants were unable to modify trophozoite attachment to Caco-2 cells. However, trophozoites treated with spent culture supernatants had little, if any, proliferative capacity. These results suggest that La1 produces some substance(s) able to inhibit proliferation of <i>Giardia</i> trophozoites. Partial characterization of the factors involved in the antigiardiasic action showed that they have a low molecular mass and are inactivated by heating. On this basis, it seems worthwhile to explore how colonization of the proximal small bowel with these lactic acid bacteria could interfere with giardiasis <i>in vivo</i>.
Facultad de Ciencias Exactas
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
description The aim of the present work was to evaluate the effect of spent culture supernatants of different strains of lactobacilli on giardia trophozoites. The growth of <i>Giardia intestinalis</i> strain WB, as well as the attachment to the human intestinal epithelial cell line Caco-2, was evaluated by using proliferation and adhesion assays with radiolabeled parasites. In addition, scanning electron microscopy and flow cytometric analysis were performed. The effect of spent culture supernatants from lactobacilli was strain dependent. <i>Lactobacillus johnsonii</i> La1 significantly inhibited the proliferation of <i>G. intestinalis</i> trophozoites. Although the effect was strongly pH dependent, it was not simply due to lactic acid. According to flow cytometric analysis, trophozoites were arrested in G<sub>1</sub> phase but neither significant necrosis nor apoptosis could be detected. Bacterial cells or their spent culture supernatants were unable to modify trophozoite attachment to Caco-2 cells. However, trophozoites treated with spent culture supernatants had little, if any, proliferative capacity. These results suggest that La1 produces some substance(s) able to inhibit proliferation of <i>Giardia</i> trophozoites. Partial characterization of the factors involved in the antigiardiasic action showed that they have a low molecular mass and are inactivated by heating. On this basis, it seems worthwhile to explore how colonization of the proximal small bowel with these lactic acid bacteria could interfere with giardiasis <i>in vivo</i>.
publishDate 2001
dc.date.none.fl_str_mv 2001
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107655
url http://sedici.unlp.edu.ar/handle/10915/107655
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC93268&blobtype=pdf
info:eu-repo/semantics/altIdentifier/url/https://aem.asm.org/content/67/11/5037
info:eu-repo/semantics/altIdentifier/issn/0099-2240
info:eu-repo/semantics/altIdentifier/pmid/11679323
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
5037-5042
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
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reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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