Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells

Autores
Molinuevo, María Silvina; Bruzzone, Liliana; Cortizo, Ana María
Año de publicación
2007
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bisphosphonates are nonhydrolysable pyrophosphate analogues that prevent bone loss in several types of cancer. However, the mechanisms of anticancer action of bisphosphonates are not completely known. We have previously shown that nitrogen-containing bisphosphonates directly inhibit alkaline phosphatase of UMR106 rat osteosarcoma cells. In this study, we evaluated the effects of alendronate on the migration of UMR106 osteosarcoma using a model of multicellular cell spheroids, as well as the alendronate effect on neutral phosphatases. Alendronate significantly inhibited the migration of osteoblasts in a dose-dependent manner (10−6–10−4 M). This effect was also dependent on calcium availability. The spheroid morphology and distribution of actin fibers were also affected by alendronate treatment. Alendronate dose-dependently inhibited neutral phosphatase activity in cell-free osteoblastic extracts as well as in osteoblasts in culture. Our results show that alendronate inhibits cell migration through mechanisms dependent on calcium, and that seem to involve inhibition of phosphotyrosine-neutral-phosphatases and disassembly of actin stress fibers.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
Materia
Biología
Spheroid
Bisphosphonates
Phosphatase
Actin fibers
Osteosarcoma
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/153967
Acceder
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network_acronym_str SEDICI
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network_name_str SEDICI (UNLP)
spelling Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cellsMolinuevo, María SilvinaBruzzone, LilianaCortizo, Ana MaríaBiologíaSpheroidBisphosphonatesPhosphataseActin fibersOsteosarcomaBisphosphonates are nonhydrolysable pyrophosphate analogues that prevent bone loss in several types of cancer. However, the mechanisms of anticancer action of bisphosphonates are not completely known. We have previously shown that nitrogen-containing bisphosphonates directly inhibit alkaline phosphatase of UMR106 rat osteosarcoma cells. In this study, we evaluated the effects of alendronate on the migration of UMR106 osteosarcoma using a model of multicellular cell spheroids, as well as the alendronate effect on neutral phosphatases. Alendronate significantly inhibited the migration of osteoblasts in a dose-dependent manner (10−6–10−4 M). This effect was also dependent on calcium availability. The spheroid morphology and distribution of actin fibers were also affected by alendronate treatment. Alendronate dose-dependently inhibited neutral phosphatase activity in cell-free osteoblastic extracts as well as in osteoblasts in culture. Our results show that alendronate inhibits cell migration through mechanisms dependent on calcium, and that seem to involve inhibition of phosphotyrosine-neutral-phosphatases and disassembly of actin stress fibers.Laboratorio de Investigación en Osteopatías y Metabolismo Mineral2007info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf28-33http://sedici.unlp.edu.ar/handle/10915/153967spainfo:eu-repo/semantics/altIdentifier/issn/0014-2999info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2007.01.054info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-17T10:22:29Zoai:sedici.unlp.edu.ar:10915/153967Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-17 10:22:29.427SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
spellingShingle Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
Molinuevo, María Silvina
Biología
Spheroid
Bisphosphonates
Phosphatase
Actin fibers
Osteosarcoma
title_short Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title_full Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title_fullStr Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title_full_unstemmed Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
title_sort Alendronate induces anti-migratory effects and inhibition of neutral phosphatases in UMR106 osteosarcoma cells
dc.creator.none.fl_str_mv Molinuevo, María Silvina
Bruzzone, Liliana
Cortizo, Ana María
author Molinuevo, María Silvina
author_facet Molinuevo, María Silvina
Bruzzone, Liliana
Cortizo, Ana María
author_role author
author2 Bruzzone, Liliana
Cortizo, Ana María
author2_role author
author
dc.subject.none.fl_str_mv Biología
Spheroid
Bisphosphonates
Phosphatase
Actin fibers
Osteosarcoma
topic Biología
Spheroid
Bisphosphonates
Phosphatase
Actin fibers
Osteosarcoma
dc.description.none.fl_txt_mv Bisphosphonates are nonhydrolysable pyrophosphate analogues that prevent bone loss in several types of cancer. However, the mechanisms of anticancer action of bisphosphonates are not completely known. We have previously shown that nitrogen-containing bisphosphonates directly inhibit alkaline phosphatase of UMR106 rat osteosarcoma cells. In this study, we evaluated the effects of alendronate on the migration of UMR106 osteosarcoma using a model of multicellular cell spheroids, as well as the alendronate effect on neutral phosphatases. Alendronate significantly inhibited the migration of osteoblasts in a dose-dependent manner (10−6–10−4 M). This effect was also dependent on calcium availability. The spheroid morphology and distribution of actin fibers were also affected by alendronate treatment. Alendronate dose-dependently inhibited neutral phosphatase activity in cell-free osteoblastic extracts as well as in osteoblasts in culture. Our results show that alendronate inhibits cell migration through mechanisms dependent on calcium, and that seem to involve inhibition of phosphotyrosine-neutral-phosphatases and disassembly of actin stress fibers.
Laboratorio de Investigación en Osteopatías y Metabolismo Mineral
description Bisphosphonates are nonhydrolysable pyrophosphate analogues that prevent bone loss in several types of cancer. However, the mechanisms of anticancer action of bisphosphonates are not completely known. We have previously shown that nitrogen-containing bisphosphonates directly inhibit alkaline phosphatase of UMR106 rat osteosarcoma cells. In this study, we evaluated the effects of alendronate on the migration of UMR106 osteosarcoma using a model of multicellular cell spheroids, as well as the alendronate effect on neutral phosphatases. Alendronate significantly inhibited the migration of osteoblasts in a dose-dependent manner (10−6–10−4 M). This effect was also dependent on calcium availability. The spheroid morphology and distribution of actin fibers were also affected by alendronate treatment. Alendronate dose-dependently inhibited neutral phosphatase activity in cell-free osteoblastic extracts as well as in osteoblasts in culture. Our results show that alendronate inhibits cell migration through mechanisms dependent on calcium, and that seem to involve inhibition of phosphotyrosine-neutral-phosphatases and disassembly of actin stress fibers.
publishDate 2007
dc.date.none.fl_str_mv 2007
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/153967
url http://sedici.unlp.edu.ar/handle/10915/153967
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0014-2999
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ejphar.2007.01.054
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
28-33
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.001348

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