Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions

Autores
López, Silvina Marianela Yanil; Balatti, Pedro Alberto
Año de publicación
2012
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
Facultad de Ciencias Agrarias y Forestales
Materia
Ciencias Agrarias
inoculant
bacterial DNA
article
molecular markers
rhizobia
artifact
bacterial strain
bacterium culture
bacterium identification
bacterium isolate
bacterium mutant
Bradyrhizobium
Bradyrhizobium elkanii
Bradyrhizobium japonicum
controlled study
DNA fingerprinting
gene amplification
inoculation
nonhuman
polymerase chain reaction
soybean
Bacteria (microorganisms)
Bradyrhizobiaceae
Glycine max
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/3.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/40776
Acceder
id SEDICI_697ce33f6c1be7f949b438648638e096
oai_identifier_str oai:sedici.unlp.edu.ar:10915/40776
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactionsLópez, Silvina Marianela YanilBalatti, Pedro AlbertoCiencias Agrariasinoculantbacterial DNAarticlemolecular markersrhizobiaartifactbacterial strainbacterium culturebacterium identificationbacterium isolatebacterium mutantBradyrhizobiumBradyrhizobium elkaniiBradyrhizobium japonicumcontrolled studyDNA fingerprintinggene amplificationinoculationnonhumanpolymerase chain reactionsoybeanBacteria (microorganisms)BradyrhizobiaceaeGlycine maxThe aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.Facultad de Ciencias Agrarias y Forestales2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/40776spainfo:eu-repo/semantics/altIdentifier/url/http://astonjournals.com/manuscripts/Vol2011/GEBJ-34_Vol2011.pdfinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/Creative Commons Attribution 3.0 Unported (CC BY 3.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T10:49:40Zoai:sedici.unlp.edu.ar:10915/40776Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 10:49:41.238SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
spellingShingle Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
López, Silvina Marianela Yanil
Ciencias Agrarias
inoculant
bacterial DNA
article
molecular markers
rhizobia
artifact
bacterial strain
bacterium culture
bacterium identification
bacterium isolate
bacterium mutant
Bradyrhizobium
Bradyrhizobium elkanii
Bradyrhizobium japonicum
controlled study
DNA fingerprinting
gene amplification
inoculation
nonhuman
polymerase chain reaction
soybean
Bacteria (microorganisms)
Bradyrhizobiaceae
Glycine max
title_short Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_full Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_fullStr Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_full_unstemmed Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_sort Closely related strains of <i>Bradyrhizobium</i> contained in commercial inoculates of soybean are identified by a set of PCR reactions
dc.creator.none.fl_str_mv López, Silvina Marianela Yanil
Balatti, Pedro Alberto
author López, Silvina Marianela Yanil
author_facet López, Silvina Marianela Yanil
Balatti, Pedro Alberto
author_role author
author2 Balatti, Pedro Alberto
author2_role author
dc.subject.none.fl_str_mv Ciencias Agrarias
inoculant
bacterial DNA
article
molecular markers
rhizobia
artifact
bacterial strain
bacterium culture
bacterium identification
bacterium isolate
bacterium mutant
Bradyrhizobium
Bradyrhizobium elkanii
Bradyrhizobium japonicum
controlled study
DNA fingerprinting
gene amplification
inoculation
nonhuman
polymerase chain reaction
soybean
Bacteria (microorganisms)
Bradyrhizobiaceae
Glycine max
topic Ciencias Agrarias
inoculant
bacterial DNA
article
molecular markers
rhizobia
artifact
bacterial strain
bacterium culture
bacterium identification
bacterium isolate
bacterium mutant
Bradyrhizobium
Bradyrhizobium elkanii
Bradyrhizobium japonicum
controlled study
DNA fingerprinting
gene amplification
inoculation
nonhuman
polymerase chain reaction
soybean
Bacteria (microorganisms)
Bradyrhizobiaceae
Glycine max
dc.description.none.fl_txt_mv The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
Facultad de Ciencias Agrarias y Forestales
description The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSα successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSα sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/40776
url http://sedici.unlp.edu.ar/handle/10915/40776
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://astonjournals.com/manuscripts/Vol2011/GEBJ-34_Vol2011.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/
Creative Commons Attribution 3.0 Unported (CC BY 3.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
_version_ 1846063928133550080
score 13.22299

Publicaciones similares