Characterized non - transient microbiota from stinkbug (Nezara viridula) midgut deactivates soybean chemical defenses

• Autores: Medina, Virginia; Sardoy, Pedro Miguel; Soria, Marcelo Abel; Vay, Carlos A.; Gutkind, Gabriel O.; Zavala, Jorge Alberto
• Año de publicación: 2018
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Plos One; Vol.13, no.7; e0200161; https://journals.plos.org
• Materia: BACTERIAL ENZYME; BETA GLUCOSIDASE; RAFFINASE; UNCLASSIFIED DRUG; BACILLUS; BACTERIUM; BACTERIUM ISOLATE; CONTROLLED STUDY; INSECT; NEZARA VIRIDULA; MICROCOCCUS
• Institución: Universidad de Buenos Aires. Facultad de Agronomía

Fil: Medina, Virginia. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Bioquímica. Buenos Aires, Argentina.
Fil: Sardoy, Pedro Miguel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Bioquímica. Buenos Aires, Argentina.
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola. Buenos Aires, Argentina.
Fil: Vay, Carlos A. Universidad de Buenos Aires. Facultad de Farmacía y Bioquímica. Buenos Aires, Argentina.
Fil: Gutkind, Gabriel O. Universidad de Buenos Aires. Facultad de Farmacía y Bioquímica. Buenos Aires, Argentina.
Fil: Zavala, Jorge Alberto. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Bioquímica. Buenos Aires, Argentina.
The Southern green stinkbug (N. viridula) feeds on developing soybean seeds in spite of their strong defenses against herbivory, making this pest one of the most harmful to soybean crops. To test the hypothesis that midgut bacterial community allows stinkbugs to tolerate chemical defenses of soybean developing seeds, we identified and characterized midgut microbiota of stinkbugs collected from soybean crops, different secondary plant hosts or insects at diapause on Eucalyptus trees. Our study demonstrated that while more than 54% of N. viridula adults collected in the field had no detectable bacteria in the V1-V3 midgut ventricles, the guts of the rest of stinkbugs were colonized by non-transient microbiota (NTM) and transient microbiota not present in stinkbugs at diapause. While transient microbiota Bacillus sp., Micrococcus sp., Streptomyces sp., Staphylococcus sp. and others had low abundance, NTM microbiota was represented by Yokenella sp., Pantoea sp. and Enterococcus sp. isolates. We found some isolates that showed in vitro ß-glucosidase and raffinase activities plus the ability to degrade isoflavonoids and deactivate soybean protease inhibitors. Our results suggest that the stinkbugs NTM microbiota may impact on nutrition, detoxification and deactivation of chemical defenses, and Enterococcus sp., Yokenella sp. and Pantoea sp. strains might help stinkbugs to feed on soybean developing seeds in spite of its chemical defenses.
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