Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen

Autores
López, Silvina Marianela Yanil; Molina Sánchez, María Dolores; Pastorino, Graciela Noemí; Franco, Mario Emilio Ernesto; Toro García, Nicolás; Balatti, Pedro Alberto
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The purpose of this work was to study further two Bradyrhizobium japonicum strains with high nitrogen-fixing capacity that were identified within a collection of approximately 200 isolates from the soils of Argentina. Nodulation and nitrogen-fixing capacity and the level of expression of regulatory as well as structural genes of nitrogen fixation and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene of the isolates were compared with that of E109-inoculated plants. Both isolates of B. japonicum, 163 and 366, were highly efficient to fix nitrogen compared to commercial strain E109. Isolate 366 developed a higher number and larger biomass of nodules and because of this fixed more nitrogen. Isolate 163 developed the same number and nodule biomass than E109. However, nodules developed by isolate 163 had red interiors for a longer period, had a higher leghemoglobin content, and presented high levels of expression of acdS gene, that codes for an ACC deaminase. In conclusion, naturalized rhizobia of the soils of Argentina hold a diverse population that might be the source of highly active nitrogen-fixing rhizobia, a process that appears to be based on different strategies.
Centro de Investigaciones en Fitopatología
Materia
Ingeniería Agronómica
Bradyrhizobium japonicum
Nitrogen
rhizobia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/140854
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/140854
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network_name_str SEDICI (UNLP)
spelling Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix NitrogenLópez, Silvina Marianela YanilMolina Sánchez, María DoloresPastorino, Graciela NoemíFranco, Mario Emilio ErnestoToro García, NicolásBalatti, Pedro AlbertoIngeniería AgronómicaBradyrhizobium japonicumNitrogenrhizobiaThe purpose of this work was to study further two Bradyrhizobium japonicum strains with high nitrogen-fixing capacity that were identified within a collection of approximately 200 isolates from the soils of Argentina. Nodulation and nitrogen-fixing capacity and the level of expression of regulatory as well as structural genes of nitrogen fixation and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene of the isolates were compared with that of E109-inoculated plants. Both isolates of B. japonicum, 163 and 366, were highly efficient to fix nitrogen compared to commercial strain E109. Isolate 366 developed a higher number and larger biomass of nodules and because of this fixed more nitrogen. Isolate 163 developed the same number and nodule biomass than E109. However, nodules developed by isolate 163 had red interiors for a longer period, had a higher leghemoglobin content, and presented high levels of expression of acdS gene, that codes for an ACC deaminase. In conclusion, naturalized rhizobia of the soils of Argentina hold a diverse population that might be the source of highly active nitrogen-fixing rhizobia, a process that appears to be based on different strategies.Centro de Investigaciones en Fitopatología2018-03-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf997-1005http://sedici.unlp.edu.ar/handle/10915/140854enginfo:eu-repo/semantics/altIdentifier/issn/1432-0991info:eu-repo/semantics/altIdentifier/issn/0343-8651info:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-018-1478-0info:eu-repo/semantics/altIdentifier/pmid/29546586info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-11-05T13:11:26Zoai:sedici.unlp.edu.ar:10915/140854Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-11-05 13:11:27.132SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen
title Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen
spellingShingle Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen
López, Silvina Marianela Yanil
Ingeniería Agronómica
Bradyrhizobium japonicum
Nitrogen
rhizobia
title_short Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen
title_full Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen
title_fullStr Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen
title_full_unstemmed Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen
title_sort Nodulation and Delayed Nodule Senescence : Strategies of Two Bradyrhizobium Japonicum Isolates with High Capacity to Fix Nitrogen
dc.creator.none.fl_str_mv López, Silvina Marianela Yanil
Molina Sánchez, María Dolores
Pastorino, Graciela Noemí
Franco, Mario Emilio Ernesto
Toro García, Nicolás
Balatti, Pedro Alberto
author López, Silvina Marianela Yanil
author_facet López, Silvina Marianela Yanil
Molina Sánchez, María Dolores
Pastorino, Graciela Noemí
Franco, Mario Emilio Ernesto
Toro García, Nicolás
Balatti, Pedro Alberto
author_role author
author2 Molina Sánchez, María Dolores
Pastorino, Graciela Noemí
Franco, Mario Emilio Ernesto
Toro García, Nicolás
Balatti, Pedro Alberto
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ingeniería Agronómica
Bradyrhizobium japonicum
Nitrogen
rhizobia
topic Ingeniería Agronómica
Bradyrhizobium japonicum
Nitrogen
rhizobia
dc.description.none.fl_txt_mv The purpose of this work was to study further two Bradyrhizobium japonicum strains with high nitrogen-fixing capacity that were identified within a collection of approximately 200 isolates from the soils of Argentina. Nodulation and nitrogen-fixing capacity and the level of expression of regulatory as well as structural genes of nitrogen fixation and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene of the isolates were compared with that of E109-inoculated plants. Both isolates of B. japonicum, 163 and 366, were highly efficient to fix nitrogen compared to commercial strain E109. Isolate 366 developed a higher number and larger biomass of nodules and because of this fixed more nitrogen. Isolate 163 developed the same number and nodule biomass than E109. However, nodules developed by isolate 163 had red interiors for a longer period, had a higher leghemoglobin content, and presented high levels of expression of acdS gene, that codes for an ACC deaminase. In conclusion, naturalized rhizobia of the soils of Argentina hold a diverse population that might be the source of highly active nitrogen-fixing rhizobia, a process that appears to be based on different strategies.
Centro de Investigaciones en Fitopatología
description The purpose of this work was to study further two Bradyrhizobium japonicum strains with high nitrogen-fixing capacity that were identified within a collection of approximately 200 isolates from the soils of Argentina. Nodulation and nitrogen-fixing capacity and the level of expression of regulatory as well as structural genes of nitrogen fixation and the 1-aminocyclopropane-1-carboxylate (ACC) deaminase gene of the isolates were compared with that of E109-inoculated plants. Both isolates of B. japonicum, 163 and 366, were highly efficient to fix nitrogen compared to commercial strain E109. Isolate 366 developed a higher number and larger biomass of nodules and because of this fixed more nitrogen. Isolate 163 developed the same number and nodule biomass than E109. However, nodules developed by isolate 163 had red interiors for a longer period, had a higher leghemoglobin content, and presented high levels of expression of acdS gene, that codes for an ACC deaminase. In conclusion, naturalized rhizobia of the soils of Argentina hold a diverse population that might be the source of highly active nitrogen-fixing rhizobia, a process that appears to be based on different strategies.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/140854
url http://sedici.unlp.edu.ar/handle/10915/140854
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/1432-0991
info:eu-repo/semantics/altIdentifier/issn/0343-8651
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00284-018-1478-0
info:eu-repo/semantics/altIdentifier/pmid/29546586
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
997-1005
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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