Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions

Autores
Balatti, Pedro Alberto; López, Silvina Marianela Yanil
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSá successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSá sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
Fil: Balatti, Pedro Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: López, Silvina Marianela Yanil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Materia
Inoculant
Molecular marker
Rhizobia
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/253781
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactionsBalatti, Pedro AlbertoLópez, Silvina Marianela YanilInoculantMolecular markerRhizobiahttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSá successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSá sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.Fil: Balatti, Pedro Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaFil: López, Silvina Marianela Yanil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; ArgentinaAston Journals2011-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/253781Balatti, Pedro Alberto; López, Silvina Marianela Yanil; Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions; Aston Journals; Biotechnology Journal Genetic Engineering and Biotechnology; 2011; 34; 12-2011; 1-82150-3516CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://sedici.unlp.edu.ar/handle/10915/40776info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:20Zoai:ri.conicet.gov.ar:11336/253781instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:20.337CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
spellingShingle Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
Balatti, Pedro Alberto
Inoculant
Molecular marker
Rhizobia
title_short Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_full Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_fullStr Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_full_unstemmed Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
title_sort Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions
dc.creator.none.fl_str_mv Balatti, Pedro Alberto
López, Silvina Marianela Yanil
author Balatti, Pedro Alberto
author_facet Balatti, Pedro Alberto
López, Silvina Marianela Yanil
author_role author
author2 López, Silvina Marianela Yanil
author2_role author
dc.subject.none.fl_str_mv Inoculant
Molecular marker
Rhizobia
topic Inoculant
Molecular marker
Rhizobia
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSá successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSá sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
Fil: Balatti, Pedro Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
Fil: López, Silvina Marianela Yanil. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Fisiología Vegetal. Universidad Nacional de La Plata. Facultad de Ciencias Naturales y Museo. Instituto de Fisiología Vegetal; Argentina
description The aim of this work was to identify closely related rhizobia, used to formulate commercial inoculants, using polymerase chain reaction (PCR). Repetitive extra-genic palindromic (REP) and BOX fingerprints hardly discriminate among a set of commercial strains. PCR targeted at repetitive RSá successfully allow discriminating within representatives of Bradyrhizobium. These fingerprints clustered isolates at a higher level of similarity and proved to be an important tool to complement the information provided by the other markers. The results suggest that mutants occur along the bacterial culture, during inoculant production. However, independently of the number of amplification reactions used to characterize and identify organisms, mispriming always generates artifactual diversity. In addition to this, it seems that combining reactions such as BOX or REP fingerprinting with reactions targeted at the RSá sequence, generates a more reliable identification tool to characterize closely related bradyrhizobia.
publishDate 2011
dc.date.none.fl_str_mv 2011-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/253781
Balatti, Pedro Alberto; López, Silvina Marianela Yanil; Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions; Aston Journals; Biotechnology Journal Genetic Engineering and Biotechnology; 2011; 34; 12-2011; 1-8
2150-3516
CONICET Digital
CONICET
url http://hdl.handle.net/11336/253781
identifier_str_mv Balatti, Pedro Alberto; López, Silvina Marianela Yanil; Closely related strains of Bradyrhizobium contained in commercial inoculates of soybean are identified by a set of PCR reactions; Aston Journals; Biotechnology Journal Genetic Engineering and Biotechnology; 2011; 34; 12-2011; 1-8
2150-3516
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://sedici.unlp.edu.ar/handle/10915/40776
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Aston Journals
publisher.none.fl_str_mv Aston Journals
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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