ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver

Autores
Mandon, Elisabet C.; Tacconi de Gómez Dumm, Irma Nelva; Tacconi de Alaniz, María Josefa; Marra, Carlos Alberto; Brenner, Rodolfo Roberto
Año de publicación
1987
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The in vivo and in vitro effect of ACTH on the biosynthesis of polyunsaturated fatty acid of rat adrenal gland and liver was studied. The administration of ACTH to intact rats produced a significant decrease in the conversion of [1-14C]linoleic acid to gamma-linolenic acid, [1-14C]alpha-linolenic acid to octadeca-6,9,12,15-tetraenoic acid, and [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid in liver and adrenal microsomes. Isolated adrenocortical cells and hepatocytes obtained from animals treated with ACTH showed a decrease in the incorporation and desaturation of exogenous [1-14C]eicosa-8,11,14-trienoic acid. The addition of ACTH to the incubation medium of adrenocortical cells and hepatocytes isolated from untreated rats also caused a decrease in delta 5 desaturation activity. The effect of ACTH on adrenal and liver desaturases could be produced as a consequence of the release of glucocorticoids, already measured in the experiments. However, the in vitro experiments carried out with hepatocytes isolated from untreated rats, where corticosterone was absent, indicated that ACTH can depress delta 5 desaturation per se.
Instituto de Investigaciones Bioquímicas de La Plata
Materia
Química
Biología
Linoleic acid
α-linolenic acid
Eicosa- 8,11,14-trienoic acid
Polyunsaturated fatty acids
Isolated adrenocortical cells
Hepatocytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/120450
Acceder
id SEDICI_5386116f24fb90d98db5a713d32c85f1
oai_identifier_str oai:sedici.unlp.edu.ar:10915/120450
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liverMandon, Elisabet C.Tacconi de Gómez Dumm, Irma NelvaTacconi de Alaniz, María JosefaMarra, Carlos AlbertoBrenner, Rodolfo RobertoQuímicaBiologíaLinoleic acidα-linolenic acidEicosa- 8,11,14-trienoic acidPolyunsaturated fatty acidsIsolated adrenocortical cellsHepatocytesThe in vivo and in vitro effect of ACTH on the biosynthesis of polyunsaturated fatty acid of rat adrenal gland and liver was studied. The administration of ACTH to intact rats produced a significant decrease in the conversion of [1-14C]linoleic acid to gamma-linolenic acid, [1-14C]alpha-linolenic acid to octadeca-6,9,12,15-tetraenoic acid, and [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid in liver and adrenal microsomes. Isolated adrenocortical cells and hepatocytes obtained from animals treated with ACTH showed a decrease in the incorporation and desaturation of exogenous [1-14C]eicosa-8,11,14-trienoic acid. The addition of ACTH to the incubation medium of adrenocortical cells and hepatocytes isolated from untreated rats also caused a decrease in delta 5 desaturation activity. The effect of ACTH on adrenal and liver desaturases could be produced as a consequence of the release of glucocorticoids, already measured in the experiments. However, the in vitro experiments carried out with hepatocytes isolated from untreated rats, where corticosterone was absent, indicated that ACTH can depress delta 5 desaturation per se.Instituto de Investigaciones Bioquímicas de La Plata1987info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf1377-1383http://sedici.unlp.edu.ar/handle/10915/120450enginfo:eu-repo/semantics/altIdentifier/issn/0022-2275info:eu-repo/semantics/altIdentifier/doi/10.1016/S0022-2275(20)38570-9info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:20:23Zoai:sedici.unlp.edu.ar:10915/120450Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:20:24.034SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver
title ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver
spellingShingle ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver
Mandon, Elisabet C.
Química
Biología
Linoleic acid
α-linolenic acid
Eicosa- 8,11,14-trienoic acid
Polyunsaturated fatty acids
Isolated adrenocortical cells
Hepatocytes
title_short ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver
title_full ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver
title_fullStr ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver
title_full_unstemmed ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver
title_sort ACTH depresses delta 6 and delta 5 desaturation activity in rat adrenal gland and liver
dc.creator.none.fl_str_mv Mandon, Elisabet C.
Tacconi de Gómez Dumm, Irma Nelva
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
Brenner, Rodolfo Roberto
author Mandon, Elisabet C.
author_facet Mandon, Elisabet C.
Tacconi de Gómez Dumm, Irma Nelva
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
Brenner, Rodolfo Roberto
author_role author
author2 Tacconi de Gómez Dumm, Irma Nelva
Tacconi de Alaniz, María Josefa
Marra, Carlos Alberto
Brenner, Rodolfo Roberto
author2_role author
author
author
author
dc.subject.none.fl_str_mv Química
Biología
Linoleic acid
α-linolenic acid
Eicosa- 8,11,14-trienoic acid
Polyunsaturated fatty acids
Isolated adrenocortical cells
Hepatocytes
topic Química
Biología
Linoleic acid
α-linolenic acid
Eicosa- 8,11,14-trienoic acid
Polyunsaturated fatty acids
Isolated adrenocortical cells
Hepatocytes
dc.description.none.fl_txt_mv The in vivo and in vitro effect of ACTH on the biosynthesis of polyunsaturated fatty acid of rat adrenal gland and liver was studied. The administration of ACTH to intact rats produced a significant decrease in the conversion of [1-14C]linoleic acid to gamma-linolenic acid, [1-14C]alpha-linolenic acid to octadeca-6,9,12,15-tetraenoic acid, and [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid in liver and adrenal microsomes. Isolated adrenocortical cells and hepatocytes obtained from animals treated with ACTH showed a decrease in the incorporation and desaturation of exogenous [1-14C]eicosa-8,11,14-trienoic acid. The addition of ACTH to the incubation medium of adrenocortical cells and hepatocytes isolated from untreated rats also caused a decrease in delta 5 desaturation activity. The effect of ACTH on adrenal and liver desaturases could be produced as a consequence of the release of glucocorticoids, already measured in the experiments. However, the in vitro experiments carried out with hepatocytes isolated from untreated rats, where corticosterone was absent, indicated that ACTH can depress delta 5 desaturation per se.
Instituto de Investigaciones Bioquímicas de La Plata
description The in vivo and in vitro effect of ACTH on the biosynthesis of polyunsaturated fatty acid of rat adrenal gland and liver was studied. The administration of ACTH to intact rats produced a significant decrease in the conversion of [1-14C]linoleic acid to gamma-linolenic acid, [1-14C]alpha-linolenic acid to octadeca-6,9,12,15-tetraenoic acid, and [1-14C]eicosa-8,11,14-trienoic acid to arachidonic acid in liver and adrenal microsomes. Isolated adrenocortical cells and hepatocytes obtained from animals treated with ACTH showed a decrease in the incorporation and desaturation of exogenous [1-14C]eicosa-8,11,14-trienoic acid. The addition of ACTH to the incubation medium of adrenocortical cells and hepatocytes isolated from untreated rats also caused a decrease in delta 5 desaturation activity. The effect of ACTH on adrenal and liver desaturases could be produced as a consequence of the release of glucocorticoids, already measured in the experiments. However, the in vitro experiments carried out with hepatocytes isolated from untreated rats, where corticosterone was absent, indicated that ACTH can depress delta 5 desaturation per se.
publishDate 1987
dc.date.none.fl_str_mv 1987
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/120450
url http://sedici.unlp.edu.ar/handle/10915/120450
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0022-2275
info:eu-repo/semantics/altIdentifier/doi/10.1016/S0022-2275(20)38570-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
1377-1383
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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