Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles

Autores
Ayala, Sixta; Gaspar, Graciela; Brenner, Rodolfo Roberto; Peluffo, Raúl Omar; Kunau, Wolf H.
Año de publicación
1973
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A comparative study was made on the fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in various subcellular fractions of liver and testis from rats of different ages. It was demonstrated that testicular microsomes can desaturate and elongate linoleic and arachidonic acids in a manner similar to liver microsomes, and that testicular mitochondria can convert docosa-7,10,13,16-tetraenoic acid to arachidonic acid. Testicular or liver microsomes actively desaturate linoleic acid to γ-linolenic acid and eicosa-8,11,14-trienoic acid to arachidonic acid. However, it was impossible to measure in vitro any direct conversion of adrenic acid (22:4 [n – 6]) to docosapentaenoic acid (22: 5 [n – 6]) by either liver or testicular microsomes. Docosa-7,10,13,16-tetraenoic acid is incorporated preferentially into the triglyceride fraction of total testis, mitochondria, and microsomes, while linoleic and arachidonic acids are incorporated more into phospholipids. The capacity of testicular microsomes, but not of liver microsomes, to synthesize polyunsaturated fatty acids declines with age. It is proposed that the synthesis of acids of the linoleic family proceeds in two stages, a rapid one in which arachidonic acid is made and a second, slower, one in which C22 and C24 acids are synthesized. In addition, there appears to be a cycle between microsomes and mitochondria that acts to conserve essential polyunsaturated C20 and C22 fatty acids by means of synthesis and partial degradation, respectively. This cycle would restrict the loss of essential fatty acids and might be of importance for the supply of arachidonic acid in testis under specific requirements and especially in older animals.
Facultad de Ciencias Médicas
Materia
Ciencias Médicas
eicosa-8,11,14-trienoic acid
docosa-4,7,10,13,16-pentaenoic acid
fatty acid synthesis
fatty acid elongation
desaturation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/122743

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network_name_str SEDICI (UNLP)
spelling Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testiclesAyala, SixtaGaspar, GracielaBrenner, Rodolfo RobertoPeluffo, Raúl OmarKunau, Wolf H.Ciencias Médicaseicosa-8,11,14-trienoic aciddocosa-4,7,10,13,16-pentaenoic acidfatty acid synthesisfatty acid elongationdesaturationA comparative study was made on the fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in various subcellular fractions of liver and testis from rats of different ages. It was demonstrated that testicular microsomes can desaturate and elongate linoleic and arachidonic acids in a manner similar to liver microsomes, and that testicular mitochondria can convert docosa-7,10,13,16-tetraenoic acid to arachidonic acid. Testicular or liver microsomes actively desaturate linoleic acid to γ-linolenic acid and eicosa-8,11,14-trienoic acid to arachidonic acid. However, it was impossible to measure in vitro any direct conversion of adrenic acid (22:4 [n – 6]) to docosapentaenoic acid (22: 5 [n – 6]) by either liver or testicular microsomes. Docosa-7,10,13,16-tetraenoic acid is incorporated preferentially into the triglyceride fraction of total testis, mitochondria, and microsomes, while linoleic and arachidonic acids are incorporated more into phospholipids. The capacity of testicular microsomes, but not of liver microsomes, to synthesize polyunsaturated fatty acids declines with age. It is proposed that the synthesis of acids of the linoleic family proceeds in two stages, a rapid one in which arachidonic acid is made and a second, slower, one in which C<sub>22</sub> and C<sub>24</sub> acids are synthesized. In addition, there appears to be a cycle between microsomes and mitochondria that acts to conserve essential polyunsaturated C<sub>20</sub> and C<sub>22</sub> fatty acids by means of synthesis and partial degradation, respectively. This cycle would restrict the loss of essential fatty acids and might be of importance for the supply of arachidonic acid in testis under specific requirements and especially in older animals.Facultad de Ciencias Médicas1973-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf296-305http://sedici.unlp.edu.ar/handle/10915/122743enginfo:eu-repo/semantics/altIdentifier/issn/0022-2275info:eu-repo/semantics/altIdentifier/pmid/9704073info:eu-repo/semantics/altIdentifier/doi/10.1016/s0022-2275(20)36887-5info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/Creative Commons Attribution 4.0 International (CC BY 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:29:14Zoai:sedici.unlp.edu.ar:10915/122743Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:29:15.078SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles
title Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles
spellingShingle Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles
Ayala, Sixta
Ciencias Médicas
eicosa-8,11,14-trienoic acid
docosa-4,7,10,13,16-pentaenoic acid
fatty acid synthesis
fatty acid elongation
desaturation
title_short Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles
title_full Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles
title_fullStr Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles
title_full_unstemmed Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles
title_sort Fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in rat testicles
dc.creator.none.fl_str_mv Ayala, Sixta
Gaspar, Graciela
Brenner, Rodolfo Roberto
Peluffo, Raúl Omar
Kunau, Wolf H.
author Ayala, Sixta
author_facet Ayala, Sixta
Gaspar, Graciela
Brenner, Rodolfo Roberto
Peluffo, Raúl Omar
Kunau, Wolf H.
author_role author
author2 Gaspar, Graciela
Brenner, Rodolfo Roberto
Peluffo, Raúl Omar
Kunau, Wolf H.
author2_role author
author
author
author
dc.subject.none.fl_str_mv Ciencias Médicas
eicosa-8,11,14-trienoic acid
docosa-4,7,10,13,16-pentaenoic acid
fatty acid synthesis
fatty acid elongation
desaturation
topic Ciencias Médicas
eicosa-8,11,14-trienoic acid
docosa-4,7,10,13,16-pentaenoic acid
fatty acid synthesis
fatty acid elongation
desaturation
dc.description.none.fl_txt_mv A comparative study was made on the fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in various subcellular fractions of liver and testis from rats of different ages. It was demonstrated that testicular microsomes can desaturate and elongate linoleic and arachidonic acids in a manner similar to liver microsomes, and that testicular mitochondria can convert docosa-7,10,13,16-tetraenoic acid to arachidonic acid. Testicular or liver microsomes actively desaturate linoleic acid to γ-linolenic acid and eicosa-8,11,14-trienoic acid to arachidonic acid. However, it was impossible to measure in vitro any direct conversion of adrenic acid (22:4 [n – 6]) to docosapentaenoic acid (22: 5 [n – 6]) by either liver or testicular microsomes. Docosa-7,10,13,16-tetraenoic acid is incorporated preferentially into the triglyceride fraction of total testis, mitochondria, and microsomes, while linoleic and arachidonic acids are incorporated more into phospholipids. The capacity of testicular microsomes, but not of liver microsomes, to synthesize polyunsaturated fatty acids declines with age. It is proposed that the synthesis of acids of the linoleic family proceeds in two stages, a rapid one in which arachidonic acid is made and a second, slower, one in which C<sub>22</sub> and C<sub>24</sub> acids are synthesized. In addition, there appears to be a cycle between microsomes and mitochondria that acts to conserve essential polyunsaturated C<sub>20</sub> and C<sub>22</sub> fatty acids by means of synthesis and partial degradation, respectively. This cycle would restrict the loss of essential fatty acids and might be of importance for the supply of arachidonic acid in testis under specific requirements and especially in older animals.
Facultad de Ciencias Médicas
description A comparative study was made on the fate of linoleic, arachidonic, and docosa-7,10,13,16-tetraenoic acids in various subcellular fractions of liver and testis from rats of different ages. It was demonstrated that testicular microsomes can desaturate and elongate linoleic and arachidonic acids in a manner similar to liver microsomes, and that testicular mitochondria can convert docosa-7,10,13,16-tetraenoic acid to arachidonic acid. Testicular or liver microsomes actively desaturate linoleic acid to γ-linolenic acid and eicosa-8,11,14-trienoic acid to arachidonic acid. However, it was impossible to measure in vitro any direct conversion of adrenic acid (22:4 [n – 6]) to docosapentaenoic acid (22: 5 [n – 6]) by either liver or testicular microsomes. Docosa-7,10,13,16-tetraenoic acid is incorporated preferentially into the triglyceride fraction of total testis, mitochondria, and microsomes, while linoleic and arachidonic acids are incorporated more into phospholipids. The capacity of testicular microsomes, but not of liver microsomes, to synthesize polyunsaturated fatty acids declines with age. It is proposed that the synthesis of acids of the linoleic family proceeds in two stages, a rapid one in which arachidonic acid is made and a second, slower, one in which C<sub>22</sub> and C<sub>24</sub> acids are synthesized. In addition, there appears to be a cycle between microsomes and mitochondria that acts to conserve essential polyunsaturated C<sub>20</sub> and C<sub>22</sub> fatty acids by means of synthesis and partial degradation, respectively. This cycle would restrict the loss of essential fatty acids and might be of importance for the supply of arachidonic acid in testis under specific requirements and especially in older animals.
publishDate 1973
dc.date.none.fl_str_mv 1973-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/122743
url http://sedici.unlp.edu.ar/handle/10915/122743
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0022-2275
info:eu-repo/semantics/altIdentifier/pmid/9704073
info:eu-repo/semantics/altIdentifier/doi/10.1016/s0022-2275(20)36887-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
Creative Commons Attribution 4.0 International (CC BY 4.0)
dc.format.none.fl_str_mv application/pdf
296-305
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