Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots

Autores
Martínez, Carolina; Petruccelli, Silvana; Giulietti, Ana María; Alvarez, María Alejandra
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 μg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 μg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 μg and 14 μg respectively.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Universidad de Buenos Aires
Materia
Bioquímica
Catalytic antibodies
In vitro cultures
Molecular farming
Recombinant antibodies
Tobacco
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/108597
Acceder
id SEDICI_5110052063edda7b77b4763962e82bb8
oai_identifier_str oai:sedici.unlp.edu.ar:10915/108597
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy rootsMartínez, CarolinaPetruccelli, SilvanaGiulietti, Ana MaríaAlvarez, María AlejandraBioquímicaCatalytic antibodiesIn vitro culturesMolecular farmingRecombinant antibodiesTobaccoNicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 μg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 μg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 μg and 14 μg respectively.Centro de Investigación y Desarrollo en Criotecnología de AlimentosUniversidad de Buenos Aires2005info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf170-176http://sedici.unlp.edu.ar/handle/10915/108597enginfo:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n2-10info:eu-repo/semantics/altIdentifier/issn/0717-3458info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/4.0/Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-09-29T11:24:36Zoai:sedici.unlp.edu.ar:10915/108597Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-09-29 11:24:37.302SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots
title Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots
spellingShingle Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots
Martínez, Carolina
Bioquímica
Catalytic antibodies
In vitro cultures
Molecular farming
Recombinant antibodies
Tobacco
title_short Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots
title_full Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots
title_fullStr Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots
title_full_unstemmed Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots
title_sort Expression of the antibody 14D9 in <i>Nicotiana tabacum</i> hairy roots
dc.creator.none.fl_str_mv Martínez, Carolina
Petruccelli, Silvana
Giulietti, Ana María
Alvarez, María Alejandra
author Martínez, Carolina
author_facet Martínez, Carolina
Petruccelli, Silvana
Giulietti, Ana María
Alvarez, María Alejandra
author_role author
author2 Petruccelli, Silvana
Giulietti, Ana María
Alvarez, María Alejandra
author2_role author
author
author
dc.subject.none.fl_str_mv Bioquímica
Catalytic antibodies
In vitro cultures
Molecular farming
Recombinant antibodies
Tobacco
topic Bioquímica
Catalytic antibodies
In vitro cultures
Molecular farming
Recombinant antibodies
Tobacco
dc.description.none.fl_txt_mv Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 μg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 μg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 μg and 14 μg respectively.
Centro de Investigación y Desarrollo en Criotecnología de Alimentos
Universidad de Buenos Aires
description Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 μg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 μg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 μg and 14 μg respectively.
publishDate 2005
dc.date.none.fl_str_mv 2005
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/108597
url http://sedici.unlp.edu.ar/handle/10915/108597
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.ejbiotechnology.info/index.php/ejbiotechnology/article/view/v8n2-10
info:eu-repo/semantics/altIdentifier/issn/0717-3458
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/4.0/
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.format.none.fl_str_mv application/pdf
170-176
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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