A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins

Autores
Julve Parreño, Jose Manuel; Huet, Estefanía; Fernández del Carmen, Asun; Segura, Alvaro; Venturi, Micol; Gandía, Antoni; Pan, Wei-Song; Albaladejo, Irene; Forment, Javier; Pla, Davinia; Wigdorovitz, Andrés; Calvete, Juan J.; Gutiérrez, Carlos; Gutiérrez, José María; Granell, Antonio; Orzáez, Diego
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Antivenoms developed from the plasma of hyperimmunized animals are the only effective treatment available against snakebite envenomation but shortage of supply contributes to the high morbidity and mortality toll of this tropical disease. We describe a synthetic biology approach to affordable and cost-effective antivenom production based on plant-made recombinant polyclonal antibodies (termed pluribodies). The strategy takes advantage of virus superinfection exclusion to induce the formation of somatic expression mosaics in agroinfiltrated plants, which enables the expression of complex antibody repertoires in a highly reproducible manner. Pluribodies developed using toxin-binding genetic information captured from peripheral blood lymphocytes of hyperimmunized camels recapitulated the overall binding activity of the immune response. Furthermore, an improved plant-made antivenom (plantivenom) was formulated using an in vitro selected pluribody against Bothrops asper snake venom toxins and has been shown to neutralize a wide range of toxin activities and provide protection against lethal venom doses in mice.
Fil: Julve Parreño, Jose Manuel. Universidad Politécnica de Valencia; España
Fil: Huet, Estefanía. Universidad Politécnica de Valencia; España
Fil: Fernández del Carmen, Asun. Universidad Politécnica de Valencia; España
Fil: Segura, Alvaro. Universidad de Costa Rica; Costa Rica
Fil: Venturi, Micol. Universidad Politécnica de Valencia; España
Fil: Gandía, Antoni. Universidad Politécnica de Valencia; España
Fil: Pan, Wei-Song. Universidad Politécnica de Valencia; España
Fil: Albaladejo, Irene. Universidad Politécnica de Valencia; España
Fil: Forment, Javier. Universidad Politécnica de Valencia; España
Fil: Pla, Davinia. Instituto de Biomedicina de Valencia; España
Fil: Wigdorovitz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Calvete, Juan J.. Instituto de Biomedicina de Valencia; España
Fil: Gutiérrez, Carlos. Universidad de Las Palmas de Gran Canaria; España
Fil: Gutiérrez, José María. Universidad de Costa Rica; Costa Rica
Fil: Granell, Antonio. Universidad Politécnica de Valencia; España
Fil: Orzáez, Diego. Universidad Politécnica de Valencia; España
Materia
MOLECULAR PHARMING
RECOMBINANT POLYCLONAL ANTIBODIES
SNAKE ANTIVENOMS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/99184
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/99184
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxinsJulve Parreño, Jose ManuelHuet, EstefaníaFernández del Carmen, AsunSegura, AlvaroVenturi, MicolGandía, AntoniPan, Wei-SongAlbaladejo, IreneForment, JavierPla, DaviniaWigdorovitz, AndrésCalvete, Juan J.Gutiérrez, CarlosGutiérrez, José MaríaGranell, AntonioOrzáez, DiegoMOLECULAR PHARMINGRECOMBINANT POLYCLONAL ANTIBODIESSNAKE ANTIVENOMShttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Antivenoms developed from the plasma of hyperimmunized animals are the only effective treatment available against snakebite envenomation but shortage of supply contributes to the high morbidity and mortality toll of this tropical disease. We describe a synthetic biology approach to affordable and cost-effective antivenom production based on plant-made recombinant polyclonal antibodies (termed pluribodies). The strategy takes advantage of virus superinfection exclusion to induce the formation of somatic expression mosaics in agroinfiltrated plants, which enables the expression of complex antibody repertoires in a highly reproducible manner. Pluribodies developed using toxin-binding genetic information captured from peripheral blood lymphocytes of hyperimmunized camels recapitulated the overall binding activity of the immune response. Furthermore, an improved plant-made antivenom (plantivenom) was formulated using an in vitro selected pluribody against Bothrops asper snake venom toxins and has been shown to neutralize a wide range of toxin activities and provide protection against lethal venom doses in mice.Fil: Julve Parreño, Jose Manuel. Universidad Politécnica de Valencia; EspañaFil: Huet, Estefanía. Universidad Politécnica de Valencia; EspañaFil: Fernández del Carmen, Asun. Universidad Politécnica de Valencia; EspañaFil: Segura, Alvaro. Universidad de Costa Rica; Costa RicaFil: Venturi, Micol. Universidad Politécnica de Valencia; EspañaFil: Gandía, Antoni. Universidad Politécnica de Valencia; EspañaFil: Pan, Wei-Song. Universidad Politécnica de Valencia; EspañaFil: Albaladejo, Irene. Universidad Politécnica de Valencia; EspañaFil: Forment, Javier. Universidad Politécnica de Valencia; EspañaFil: Pla, Davinia. Instituto de Biomedicina de Valencia; EspañaFil: Wigdorovitz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; ArgentinaFil: Calvete, Juan J.. Instituto de Biomedicina de Valencia; EspañaFil: Gutiérrez, Carlos. Universidad de Las Palmas de Gran Canaria; EspañaFil: Gutiérrez, José María. Universidad de Costa Rica; Costa RicaFil: Granell, Antonio. Universidad Politécnica de Valencia; EspañaFil: Orzáez, Diego. Universidad Politécnica de Valencia; EspañaWiley Blackwell Publishing, Inc2018-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/99184Julve Parreño, Jose Manuel; Huet, Estefanía; Fernández del Carmen, Asun; Segura, Alvaro; Venturi, Micol; et al.; A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins; Wiley Blackwell Publishing, Inc; Plant Biotechnology Journal; 16; 3; 3-2018; 727-7361467-7644CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12823info:eu-repo/semantics/altIdentifier/doi/10.1111/pbi.12823info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:37Zoai:ri.conicet.gov.ar:11336/99184instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:37.579CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins
title A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins
spellingShingle A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins
Julve Parreño, Jose Manuel
MOLECULAR PHARMING
RECOMBINANT POLYCLONAL ANTIBODIES
SNAKE ANTIVENOMS
title_short A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins
title_full A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins
title_fullStr A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins
title_full_unstemmed A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins
title_sort A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins
dc.creator.none.fl_str_mv Julve Parreño, Jose Manuel
Huet, Estefanía
Fernández del Carmen, Asun
Segura, Alvaro
Venturi, Micol
Gandía, Antoni
Pan, Wei-Song
Albaladejo, Irene
Forment, Javier
Pla, Davinia
Wigdorovitz, Andrés
Calvete, Juan J.
Gutiérrez, Carlos
Gutiérrez, José María
Granell, Antonio
Orzáez, Diego
author Julve Parreño, Jose Manuel
author_facet Julve Parreño, Jose Manuel
Huet, Estefanía
Fernández del Carmen, Asun
Segura, Alvaro
Venturi, Micol
Gandía, Antoni
Pan, Wei-Song
Albaladejo, Irene
Forment, Javier
Pla, Davinia
Wigdorovitz, Andrés
Calvete, Juan J.
Gutiérrez, Carlos
Gutiérrez, José María
Granell, Antonio
Orzáez, Diego
author_role author
author2 Huet, Estefanía
Fernández del Carmen, Asun
Segura, Alvaro
Venturi, Micol
Gandía, Antoni
Pan, Wei-Song
Albaladejo, Irene
Forment, Javier
Pla, Davinia
Wigdorovitz, Andrés
Calvete, Juan J.
Gutiérrez, Carlos
Gutiérrez, José María
Granell, Antonio
Orzáez, Diego
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MOLECULAR PHARMING
RECOMBINANT POLYCLONAL ANTIBODIES
SNAKE ANTIVENOMS
topic MOLECULAR PHARMING
RECOMBINANT POLYCLONAL ANTIBODIES
SNAKE ANTIVENOMS
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Antivenoms developed from the plasma of hyperimmunized animals are the only effective treatment available against snakebite envenomation but shortage of supply contributes to the high morbidity and mortality toll of this tropical disease. We describe a synthetic biology approach to affordable and cost-effective antivenom production based on plant-made recombinant polyclonal antibodies (termed pluribodies). The strategy takes advantage of virus superinfection exclusion to induce the formation of somatic expression mosaics in agroinfiltrated plants, which enables the expression of complex antibody repertoires in a highly reproducible manner. Pluribodies developed using toxin-binding genetic information captured from peripheral blood lymphocytes of hyperimmunized camels recapitulated the overall binding activity of the immune response. Furthermore, an improved plant-made antivenom (plantivenom) was formulated using an in vitro selected pluribody against Bothrops asper snake venom toxins and has been shown to neutralize a wide range of toxin activities and provide protection against lethal venom doses in mice.
Fil: Julve Parreño, Jose Manuel. Universidad Politécnica de Valencia; España
Fil: Huet, Estefanía. Universidad Politécnica de Valencia; España
Fil: Fernández del Carmen, Asun. Universidad Politécnica de Valencia; España
Fil: Segura, Alvaro. Universidad de Costa Rica; Costa Rica
Fil: Venturi, Micol. Universidad Politécnica de Valencia; España
Fil: Gandía, Antoni. Universidad Politécnica de Valencia; España
Fil: Pan, Wei-Song. Universidad Politécnica de Valencia; España
Fil: Albaladejo, Irene. Universidad Politécnica de Valencia; España
Fil: Forment, Javier. Universidad Politécnica de Valencia; España
Fil: Pla, Davinia. Instituto de Biomedicina de Valencia; España
Fil: Wigdorovitz, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Genética; Argentina
Fil: Calvete, Juan J.. Instituto de Biomedicina de Valencia; España
Fil: Gutiérrez, Carlos. Universidad de Las Palmas de Gran Canaria; España
Fil: Gutiérrez, José María. Universidad de Costa Rica; Costa Rica
Fil: Granell, Antonio. Universidad Politécnica de Valencia; España
Fil: Orzáez, Diego. Universidad Politécnica de Valencia; España
description Antivenoms developed from the plasma of hyperimmunized animals are the only effective treatment available against snakebite envenomation but shortage of supply contributes to the high morbidity and mortality toll of this tropical disease. We describe a synthetic biology approach to affordable and cost-effective antivenom production based on plant-made recombinant polyclonal antibodies (termed pluribodies). The strategy takes advantage of virus superinfection exclusion to induce the formation of somatic expression mosaics in agroinfiltrated plants, which enables the expression of complex antibody repertoires in a highly reproducible manner. Pluribodies developed using toxin-binding genetic information captured from peripheral blood lymphocytes of hyperimmunized camels recapitulated the overall binding activity of the immune response. Furthermore, an improved plant-made antivenom (plantivenom) was formulated using an in vitro selected pluribody against Bothrops asper snake venom toxins and has been shown to neutralize a wide range of toxin activities and provide protection against lethal venom doses in mice.
publishDate 2018
dc.date.none.fl_str_mv 2018-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/99184
Julve Parreño, Jose Manuel; Huet, Estefanía; Fernández del Carmen, Asun; Segura, Alvaro; Venturi, Micol; et al.; A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins; Wiley Blackwell Publishing, Inc; Plant Biotechnology Journal; 16; 3; 3-2018; 727-736
1467-7644
CONICET Digital
CONICET
url http://hdl.handle.net/11336/99184
identifier_str_mv Julve Parreño, Jose Manuel; Huet, Estefanía; Fernández del Carmen, Asun; Segura, Alvaro; Venturi, Micol; et al.; A synthetic biology approach for consistent production of plant-made recombinant polyclonal antibodies against snake venom toxins; Wiley Blackwell Publishing, Inc; Plant Biotechnology Journal; 16; 3; 3-2018; 727-736
1467-7644
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12823
info:eu-repo/semantics/altIdentifier/doi/10.1111/pbi.12823
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268983834705920
score 13.13397

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