The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer...

Autores
Marconi, Patricia Laura; Alvarez, Maria Alejandra
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The effect of two protein stabilizers (polyvinylpyrrolidone [PVP] and gelatine) on growth and 14D9 yield of Nicotiana tabacum cell suspension cultures (Ab-KDEL and sec-Ab) was analyzed. The addition of PVP at a concentration of 1.0 g L−1 produced the highest total 14D9 yield (biomass + culture medium) in the Ab-KDEL line (4.82% total soluble protein [TSP]). With the addition of gelatine, the highest total 14D9 yield (2.48% TSP) was attained in the Ab-KDEL line at 5.0 g L−1 gelatine. When the Ab-KDEL suspended cells were cultured in a 2-L bioreactor, the highest 14D9 yield was 8.1% TSP at a 5% w/v inoculum size, which was the best 14D9 yield so far obtained in the platforms tested (E. coli, N. tabacum leaves and seeds, N. tabacum hairy roots, and cell suspension cultures).
Fil: Marconi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Facultad de Ciencias de la Salud. Carreras de Farmacia y Bioquímica. Cátedra de Farmacobotánica y Farmacognoscia; Argentina
Fil: Alvarez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Facultad de Ciencias de la Salud. Carreras de Farmacia y Bioquímica. Cátedra de Farmacobotánica y Farmacognoscia; Argentina
Materia
Catalytic Antibody 14d9
Cell Suspension Cultures
Hairy Roots
In Vitro Plant Cell Cultures
Nicotiana Tabacum
Protein Additives
Bioreactor
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33713
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/33713
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactorMarconi, Patricia LauraAlvarez, Maria AlejandraCatalytic Antibody 14d9Cell Suspension CulturesHairy RootsIn Vitro Plant Cell CulturesNicotiana TabacumProtein AdditivesBioreactorhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4The effect of two protein stabilizers (polyvinylpyrrolidone [PVP] and gelatine) on growth and 14D9 yield of Nicotiana tabacum cell suspension cultures (Ab-KDEL and sec-Ab) was analyzed. The addition of PVP at a concentration of 1.0 g L−1 produced the highest total 14D9 yield (biomass + culture medium) in the Ab-KDEL line (4.82% total soluble protein [TSP]). With the addition of gelatine, the highest total 14D9 yield (2.48% TSP) was attained in the Ab-KDEL line at 5.0 g L−1 gelatine. When the Ab-KDEL suspended cells were cultured in a 2-L bioreactor, the highest 14D9 yield was 8.1% TSP at a 5% w/v inoculum size, which was the best 14D9 yield so far obtained in the platforms tested (E. coli, N. tabacum leaves and seeds, N. tabacum hairy roots, and cell suspension cultures).Fil: Marconi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Facultad de Ciencias de la Salud. Carreras de Farmacia y Bioquímica. Cátedra de Farmacobotánica y Farmacognoscia; ArgentinaFil: Alvarez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Facultad de Ciencias de la Salud. Carreras de Farmacia y Bioquímica. Cátedra de Farmacobotánica y Farmacognoscia; ArgentinaWiley2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33713Marconi, Patricia Laura; Alvarez, Maria Alejandra; The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor; Wiley; Biotechnology Progress; 30; 5; 7-2014; 1185-11898756-79381520-6033CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1940/abstractinfo:eu-repo/semantics/altIdentifier/doi/10.1002/btpr.1940info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:15Zoai:ri.conicet.gov.ar:11336/33713instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:15.867CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor
title The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor
spellingShingle The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor
Marconi, Patricia Laura
Catalytic Antibody 14d9
Cell Suspension Cultures
Hairy Roots
In Vitro Plant Cell Cultures
Nicotiana Tabacum
Protein Additives
Bioreactor
title_short The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor
title_full The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor
title_fullStr The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor
title_full_unstemmed The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor
title_sort The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor
dc.creator.none.fl_str_mv Marconi, Patricia Laura
Alvarez, Maria Alejandra
author Marconi, Patricia Laura
author_facet Marconi, Patricia Laura
Alvarez, Maria Alejandra
author_role author
author2 Alvarez, Maria Alejandra
author2_role author
dc.subject.none.fl_str_mv Catalytic Antibody 14d9
Cell Suspension Cultures
Hairy Roots
In Vitro Plant Cell Cultures
Nicotiana Tabacum
Protein Additives
Bioreactor
topic Catalytic Antibody 14d9
Cell Suspension Cultures
Hairy Roots
In Vitro Plant Cell Cultures
Nicotiana Tabacum
Protein Additives
Bioreactor
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The effect of two protein stabilizers (polyvinylpyrrolidone [PVP] and gelatine) on growth and 14D9 yield of Nicotiana tabacum cell suspension cultures (Ab-KDEL and sec-Ab) was analyzed. The addition of PVP at a concentration of 1.0 g L−1 produced the highest total 14D9 yield (biomass + culture medium) in the Ab-KDEL line (4.82% total soluble protein [TSP]). With the addition of gelatine, the highest total 14D9 yield (2.48% TSP) was attained in the Ab-KDEL line at 5.0 g L−1 gelatine. When the Ab-KDEL suspended cells were cultured in a 2-L bioreactor, the highest 14D9 yield was 8.1% TSP at a 5% w/v inoculum size, which was the best 14D9 yield so far obtained in the platforms tested (E. coli, N. tabacum leaves and seeds, N. tabacum hairy roots, and cell suspension cultures).
Fil: Marconi, Patricia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Facultad de Ciencias de la Salud. Carreras de Farmacia y Bioquímica. Cátedra de Farmacobotánica y Farmacognoscia; Argentina
Fil: Alvarez, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Maimónides. Facultad de Ciencias de la Salud. Carreras de Farmacia y Bioquímica. Cátedra de Farmacobotánica y Farmacognoscia; Argentina
description The effect of two protein stabilizers (polyvinylpyrrolidone [PVP] and gelatine) on growth and 14D9 yield of Nicotiana tabacum cell suspension cultures (Ab-KDEL and sec-Ab) was analyzed. The addition of PVP at a concentration of 1.0 g L−1 produced the highest total 14D9 yield (biomass + culture medium) in the Ab-KDEL line (4.82% total soluble protein [TSP]). With the addition of gelatine, the highest total 14D9 yield (2.48% TSP) was attained in the Ab-KDEL line at 5.0 g L−1 gelatine. When the Ab-KDEL suspended cells were cultured in a 2-L bioreactor, the highest 14D9 yield was 8.1% TSP at a 5% w/v inoculum size, which was the best 14D9 yield so far obtained in the platforms tested (E. coli, N. tabacum leaves and seeds, N. tabacum hairy roots, and cell suspension cultures).
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33713
Marconi, Patricia Laura; Alvarez, Maria Alejandra; The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor; Wiley; Biotechnology Progress; 30; 5; 7-2014; 1185-1189
8756-7938
1520-6033
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33713
identifier_str_mv Marconi, Patricia Laura; Alvarez, Maria Alejandra; The expression of the 14D9 catalytic antibody in suspended cells of Nicotiana tabacum cultures increased by the addition of protein stabilizers and by transference from erlenmeyer flasks to a 2-L bioreactor; Wiley; Biotechnology Progress; 30; 5; 7-2014; 1185-1189
8756-7938
1520-6033
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/btpr.1940/abstract
info:eu-repo/semantics/altIdentifier/doi/10.1002/btpr.1940
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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